...or postdoc forever, and you need to be thinking about what comes next. To that end, one of the most important...personal connection; the importance of which will be elaborated on later. As for what job fairs to consider, ...the specific position he or she originally called about, you can keep in touch and reach back out when you...

...usage bias Read more about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic... out Addgene's Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection...

...Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR enzyme Cpf1 ... and specific? The two AAV strategies described above showed successful target modification, indicating...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's... a cell. The Goedhart lab, Gadella lab, and collaborators have an ongoing effort to improve genetically..., that are optimized for excitation wavelengths above 1,000 nm and enable two-color imaging with red fluorescence...

...much information as possible from depositing laboratories and associated publications, but our quality...the process. Another design choice we appreciated about Nextflow was how effortless it was for us to switch...interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our pipeline...

...proteins or nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation ...proteins in an IP are not denatured (learn more about antibodies here!) When using Protein A/G to immobilize...eluted by boiling the beads in SDS. (To learn more about Western blotting check out our blog post The Basics...

... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...generated from lentiviral preparations (described above) that are subject to a concentration step prior ...

...In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. ...apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David Liu and Dali Li labs reported... CRISPR 101: Cytosine Transversion Editors Read about four base editor reporters Resources on Addgene.org...

...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...stepwise, achieving mutation efficiencies of up to about 9% of colonies (102 to 104 times higher than traditional...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

... negative regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single...copy number and compensating for the additional metabolic cost imposed on the host with a beneficial function.... On the other hand, they also impose a lower metabolic cost on cells and produce lower amounts of protein...

...series of one- or two-marker plots, like in Figure 3 above, until the population of interest has been successfully...  Of course, there are many more things to learn about when diving into flow cytometry data. There’s different... ways to use FSC and SSC, as briefly alluded to above. There’s many variations in how one can gate, select...

... on the Addgene Blog Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs...expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and...

...following unanswered questions about CRISPR screen design. Unanswered questions about genome-wide CRISPR/Cas9...Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting ...

...of the important considerations you should think about when developing optogenetics behavioral experiments...scientific research is that the field benefits from collaboration and the exchange of ideas. The rapid spread ...versatility and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this...

...How does inducible expression work? As mentioned above, many expression plasmids utilize inducible promoters...see protein overexpression? The strains described above should generate sufficient expression levels for...may help with solubility issues. We want to hear about your favorite protein expression tips and tricks...

... a sucrase that converts sucrose into a toxic metabolite. Cells that have undergone a double crossover...confirmation of successful single crossover – after about 48 hours, the resulting colonies turn subtly blue...blog posts Find our CRISPR blog posts Learn more about Lambda Red recombineering Resources on Addgene....

...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...reporters and biosensors NIR reporters can tell us about cellular events via  fluorescence changes occurring...NIR-GECOs were developed based on mIFP6. Learn more about the characteristics, performance, and applications...

...abundant in a sample. Whereas Southern blotting is labor intensive and requires a large amount of high-quality...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...PMC4411503. Resources on the Addgene Blog Learn More about Restriction Cloning Get Tips on Analyzing DNA Sequencing...

...the annealed and filled-in gaps The great part about this mix of enzymes is that they can all work at...the standard Gibson assembly techniques described above, this resulted in the direct and seamless cloning...Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques Additional Resources on...

...estimated that very little dystrophin correction (about 4%) is needed to see muscle improvement, with only...diseases we’d like to treat with CRISPR. As seen above, DMD can be treated with “one-size-fits-most” NHEJ-mediated.... Precision genome editing faces the challenges above and more. One chief challenge is upregulating HDR...

...certain cellular behaviors, but little is known about the order or timing of these factors. Recombinase-based... three methods of state interrogation mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based...Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from Grad Student to SynBio...

...really passionate about during my degree. I loved parasitology. However, when I enquired about roles in this...with an overview of my experience and how I felt about the different positions to enable them to decide...

...2012 Mar 20;109(12):E690-7. For further reading about SpyLigase technology, read the Addgene interview...; DOI: 10.1021/sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research...in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN...pGreen-IIS based destination vectors. To learn more about the GreenGate cloning system, see the detailed plasmid...

...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...the captured proteins off for mass spec analysis (above). When the results come back it shows that the majority...our Selecting an Antibody blog post! Learn more about Antibodies and their many applications in our Antibodies...

...learn more about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design...interest, I encountered low efficiency (0.13%) and laborious handling (screening 768 F1 animals). Recovering...

...but it’s real! In this post, we’re going to talk about the power and flexibility of self-labeling protein...ready-to-use without any cloning at all! As mentioned above, several variants of these tags have been developed...Illuminating Choices blog post for more discussion about choosing a dye or ligand to use for your experiment...

... for their use. Then, we’ll provide information about commonly used and emerging capsids to help you decide...our Caltech Systemic Capsids page to learn more about that collection. Table 1: Systemic capsid plasmids...slices Several of the systemic capsids mentioned above have also been tested on ex vivo brain slices obtained...experiments and Addgene’s AAV Data Hub to learn more about the capsid you’re interested in. Then request your...

...curriculum. From the details about personal protective equipment to learning about how to use a tissue culture...

...but be sure to let us know if you'd like to write about your plasmids in a future blog post. No time to ...repository. These variants can be used as tools to learn about the influence of structure on an FP’s properties...100bp or 1kb fragments are produced (see figure above). 3 Reasons The PSU Ladders Are Awesome Tools: ...

...working in a lab for the first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing...resources Use our Plasmids 101 eBook to teach students about basic plasmid biology Use or CRISPR 101 eBook to...

... Box Find 5 Smart Phone Apps for Lab Life Learn About the Evolution of Lab Techniques Resources on Addgene.org...Digests Make Your Agarose Gel Other Resources Read about Gel Stains on the BitesizeBio Blog ...

...gene editing: Guest blogger Qichen Yuan tells us about a new tool for efficient multiplex base editing ...incredible science - I hope you've enjoyed learning about it (and maybe even picked up a new technique or ...

...Program (HuBMAP)  Antibody Characterization Laboratory Cancer Any Data repository From the Frederick...And if you're interested in joining discussions about improving antibody quality throughout research, ...

...Additional Resources on the Addgene Blog Learn More About Alex's Work with Cas9 Activators Michael Koeris ...Pages Find Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership Team ...

...repository’s future. If you’ve ever been curious about what it takes to start your own nonprofit, this ...Harvard University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge...

...faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment that exists in ...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...

...presented by the submitting scientists, learn more about the AAV Data Hub, and hear the incredible AAV talks...DOI. Reports that meet our minimum standards (* above) will be given a DOI for easy citation by others...

...to picture (see the picture of our shipping room above), but as Joanne Kamens, Addgene’s executive director...A colossal 547 years! While we are very excited about reaching this milestone, it wouldn’t have been possible...

..., I learned a lot about how FRET can be used to study biological phenomena and about how fluorophores ...

...antibodies to be used as a powerful reagent in the laboratory.   The DSHB focuses on monoclonal antibodies,...Hybridoma Bank (DSHB) since 1996.       More Blog Posts about Scientific Sharing: The 10 Most Distributed Plasmid...

...Database. Step 2: Insert name  Include information about the insert in your plasmid name. This is often a... just by reading the name. For more information about plasmid features and tips you can use in the lab...

...name from Novophage to Sample 6. To learn more about Novophage's successful transformation into Sample...to focus on a technology that one is passionate about developing? 38:00 - End : Dr. Koeris' role on the...

...scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside of the...distribute their plasmids. The partnerships and collaborations with institutions and tech transfer offices...

...Of course, all of you toiling away in laboratories this holiday season want the work you are doing to... has to do with online accessibility. It is now about as easy to find a paper in a lesser-known journal...

...re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting.   ...can be informative, so spend some time thinking about which type of gradient can help answer your particular...overexpression. Before starting a western blot, think about how you’ll interpret the data and what level of ...

...straight from the new search bar, but the menu just above it can help you quickly and easily navigate to educational...down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit, request...

...cells are avaliable for distribution. To learn more about pooled library screening, read our recent blog post...the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common...

...takes a lot of time and energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags...Pubmed PMID: 23435261. Resources at Addgene Read about other Protein Tags in our Plasmids 101 Series. Get...

...Agreement (MTA) matters: Science thrives on collaboration (it’s the heart and soul of Addgene’s mission...scientists are sharing their materials. Questions about intellectual property rights, pending publications...

...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...