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Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Adeno-associated Virus (AAV) is used extensively for in-vivo transgene expression. Learn how to use AAV in this post.

A growing area of scientific communication is preprints, a strong open science resource explored in this post.

An overview of non-antibody affinity reagents such as Fab fragments, diabodies, and single-chain fragment variables

Get advice on tagging optogenetics and chemogenetics receptors to ensure that your receptors express and/or localize to the membrane.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

A paper published in Nature used CRISPR to correct a disease-causing mutation in human embryos. Does this paper herald a future of human germline editing?

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Long-read sequencing has allowed researchers to identify plasmid dimers and multimers. Learn about them - and how to troubleshoot - in this blog post.

When you have multiple antibodies to choose from, does isotype matter? Yes, and understanding how can help you pick the right antibodies for your experiment.

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently available in lentiviral format.

Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for affinity purification (TRAP).

Learn about the most common injection routes in mice, including their key differences and uses.