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Showing: 241 - 260 of 437 results

  2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...

  3. Viral Production at Addgene

    Type
    Blog Post
    ... to AAV References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination...doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of Lentiviral...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...References and Resources References Bangera, G., Brownell, S. 2014. Course-based undergraduate research experiences...Sciences Education. 15(4):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even temperature change... all of the droplets, but you can go as low as 1C/s. Increasing the elongation time to 2 minutes and the...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ... the genome to introduce the genetic modification(s) contained within it by selecting for Zeocin resistance...19416369. Kulakauskas, Saulius, Arvydas Lubys, and S. Dusko Ehrlich. "DNA restriction-modification systems...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22....

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...transferable. Use your experience and your passion(s) to guide you in building a grant career. Do you have... client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor, find...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... menschlichen und tierischen Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky....1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et ...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ... peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant...Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility and Affinity Abundance...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015)....al., 2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression...//doi.org/10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling...

  13. Retrograde AAV viral preps

    Type
    Collection
    ...50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA Syn ...

  15. AAV Molecular Tools

    Type
    Collection
    ...Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent...

  17. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic...Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...nucleotides of DNA can serve as the target for Cas9’s single guide RNA (sgRNA), provided it meets two conditions...system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted...

  19. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

  20. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences,...They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t integrate or replicate...from this reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation...
Showing: 241 - 260 of 437 results