We narrowed to 339 results for: ADA

1. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   ... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...

2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   ...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...

3. Antibodies 101: Isotypes

   Type

   Blog Post

   ... constant domains. This switching helps B cells adapt during the course of an immune response since it...

4. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   ...10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced...

5. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   ...Nature 560.7717 (2018): 248. PubMed PMID: 30069054. Sadanand, Saheli. "EvolvR-ing to targeted mutagenesis."...

6. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   ... dsRNAs target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically...

7. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   ...in science communication from across the US and Canada convene at ComSciCon's annual flagship workshop...

8. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   ...RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased EsCas13d...

9. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   ...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...

10. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    ...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4...

11. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    ...’re not at your computer. Maps, sequences, & downloadable .gb & .dna files Once you’ve identified a plasmid...

12. Hot Plasmids - February 2022

    Type

    Blog Post

    ...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...

13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

14. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    ...Repression and Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

15. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    ...ardor and attended to with diligence.” - Abigail Adams, 1780 There are potential mentors all around you...

16. All in a Twist: dsRNA

    Type

    Blog Post

    ...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...

17. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    ...cells promote oxidative stress leading to the degradation of one key enzyme (among others): caveolin 1,...

18. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    ...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins...

19. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    ...provided by the major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but...

20. Summer SciComm: Preprints

    Type

    Blog Post

    ...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...