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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 712 results for: BASE

Showing: 241 - 260 of 712 results

  1. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...and what work he's planning on doing with CRISPR-based gene drives focusing on safety and the ability to...Board with a particular interest in generating Cas9 based tools to enable facile control of DNA and RNA.   ...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...into a maxi-prep culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep for...another transfection method such as cationic liposome-based transfection. Optimize transfection conditions for...Optimize PCR conditions for each primer pair designed based on testing the bulk sorted cells. Run samples on...

  3. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ... knowledge base is going to necessarily need a diverse group of people to provide that base. Sometimes... Mission accomplished! One example of a mission-based team in academia would be your school’s post-doc...Let’s think about some of the advantages of team-based work: Nobody knows everything anymore John F. Kennedy... post, so here are just a few critical features based on my own experience: Leadership Few things are ...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...infectious titer of a viral vector stock can vary based on transduction target and can also be altered by...titer is typically calculated by two popular PCR-based methods: Quantitative PCR (qPCR) Digital droplet...not widely used today (Fagone et al., 2012). PCR-based methods are robust, easy, fast, and convenient. ...quantified with a different primer pair. Ct values vary based on the amount of starting material in the PCR because...

  5. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ... et al) we were very excited to set up a CRISPR-based editing platform in our lab. Our goal was to use...binding sequence, we recommend changing several bases as close to the PAM as possible (see below to find...Ludwig-Maximilians-University Munich. His lab develops iPSC-based models of neurodegenerative and neurovascular diseases...of Alzheimer’s disease by developing novel iPSC-based model systems.     References 1. Cong, Le, et al...

  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...are functionally differentiated from one another. Based on their structural elements, oris interact with...proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility groups ...campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1  Gram-negative  Alcaligenes eutrophus...constitutive and IPTG-inducible integron promoter-based expression systems for Escherichia coli and Agrobacterium...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers to successfully ...a minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction...feature used in TA-cloning). Such non-complementary bases at the 3’-end interfere with plasmid reconstitution...et al. "Predicting DNA duplex stability from the base sequence." Proceedings of the National Academy of...

  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...the best possible results for any tissue culture-based experiment, labs should routinely test for mycoplasma...detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...concentration and titration of pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009):...

  9. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... decisions based on experimental timelines and specific aims. 2. Then make decisions based on financial...instructions on creating a budget, making decisions based on that budget, and negotiating in regards to the...will need, and how long it will take to publish based on these findings. Most often we suggest estimating...

  10. Chemogenetics Plasmids

    Type
    Collection
    ... KORD. hM3D(Gq), rM3D(Gs), and hM4D(Gi) are all based on muscarinic receptors and couple to the Gi, Gq...

  11. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene...

  12. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ... performing either PCR - or restriction enzyme -based cloning to test individual clones before use of ... what it should be. A useful restriction enzyme based technique for verifying plasmids like this is "plasmid...

  13. Centrifugation

    Type
    Protocol
    ...type of centrifuge needed. This decision should be based on the size of tubes you are using and the speed...than water, then you may need to fill the blank based on weight instead of volume. Pro-Tip For microfuge...

  14. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...of imaging data (ILASTIK), or the popular plugin-based image process framework FIJI are just three of the...to the biological protocol, and assembly designs based around the use of commercially available tools and...provides a potential conflict between traditional IP-based models and the use of taxpayer money to support ...

  15. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...allows classification of individual target molecules based on their mutation status. The drop-off probe is ...References 1. Miyaoka, Y. et al. Isolation of single-base genome-edited human iPS cells without antibiotic..., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient and Highly Specific Screening...

  16. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...contributed by guest blogger, Rachel Rubinstein, a field-based software product manager at Oxford Nanopore Technologies... messy if you’re not on top of it. In a science-based software PM role, having a good understanding of...Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore Technologies ...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... efficiencies ranking as low, medium or high is based on the reprogramming efficiencies reported in González...Non-replicating Plasmids: Generating iPSCs with plasmid-based expression requires serial transfection of 1 or ...Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids carry the origin of replication...DNA methylation. Another downside of using EBNA1 based vectors is that the plasmid can hang around for ...behave. iPSCs are typically tested with molecular-based assays first. These methods less rigorously assesses...Transgene Expression Mediated by a Sendai Virus-Based Vector Using a Nonstructural C Protein and Endogenous...

  20. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ... your website? Many vendors will process orders based on a purchase order, which is an official document...depositing institutions. Addgene cannot fulfill orders based on PO documents that are sent to us by fax or email...
Showing: 241 - 260 of 712 results