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Showing: 241 - 260 of 562 results
  1. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...nervous system (CNS) orchestrates complex processes enabling organisms to control their movements and behavior...opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas by Bobby...
  2. Transferable Skills Guide: External Collaborations

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    Blog Post
    ...implementation, and now must focus on managing the established partnership. If you’re willing to do similar ...some advice and guidance. The relationships you establish in this way can, of course, lead to jobs and even...
  3. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...Janelia Research Campus on the GENIE website.   Bibliography  Ersing, I., Rego, M., Wang, C., Zhang, Y., ...
  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...precipitate. GUS is usually the first choice for establishing a new transformation protocol as it can easily...might help to get you started. Once you have established a well working protocol in your lab, it can become...
  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... vehicle for making research data available for public access, review, and query. Gates Open Research ... both accept deposit of data associated with publications into one of five general purpose open source...
  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just...
  7. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK...
  8. Transferable Skills Guide: Creativity

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    Blog Post
    ...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face...
  9. Bioinformatics at Addgene

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    Blog Post
    ... from depositing laboratories and associated publications, but our quality control (QC) standards have...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...
  10. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post
    ...our loved ones, and become a positive force for public health. Visit Addgene's Microbiology Pages What...about health equity, science communication, and public health.     References 1. O'Neill, J. "Tackling...
  11. Healthcare Consulting: A Door to the Business of Life Sciences

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    Blog Post
    ...develop communication strategies, or understand, and publish on the health and economic benefits of novel drugs...conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech, and BioCentury...
  12. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...
  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...be delivered in a single package. In a recent publication, the Liu lab showed that this method of delivery... to partially correct mouse models of genetic blindness (An et al., 2024) — the first demonstrated use...
  14. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...actively interested in science communication and enabling access to science in developing countries. You...diagnostics. https://www.broadinstitute.org/files/publications/special/COVID-19%20detection%20(updated).pdf...
  15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the..., we highly recommend reading the associated publications or protocols to get the most out of the plasmid...
  16. CRISPR Plasmids - Bacteria

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    Collection
    ...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including...the entire population. ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) ...
  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...the images are accessible to all viewers. Use a colorblind-friendly color palette for display (like green-magenta-blue...informative image-based figures for scientific publications. PLOS Biology, 19(3), e3001161. https://doi....
  18. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...and incubation times. If the vendor includes publications that cited the antibody, consider reviewing ... the materials and methods sections of those publications.  Finally, always verify that the chosen antibody...
Showing: 241 - 260 of 562 results