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A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

A general overview of how and when to use screens vs. selections and the ins and outs of each method.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Learn about the Reproducibility for Everyone collaboration and how you can bring reproducibility workshops to your conference or university.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

The advantages of participating in a peer mentoring group for scientists and how to form your own.

Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana Gradinaru's lab at Caltech.

Get free science-themed Zoom backgrounds from Addgene! Backgrounds include plasmids, viruses, DNA, and more.

Learn about the Professional Development Hub's work to support science trainee career transitions. Register for the event covering findings from their report!

Use these protocols and tips to make your own competent cells and transform your DNA. It's easy and affordable.

Learn all about plasmid pooled libraries, how to amplify and use them, and what types of experiments can be done with a pooled library.

Curious about what an ITR is and why it's so important for an AAV? Learn all about inverted terminal repeats in this Viral Vectors 101 post.

Considering purifying a protein to study it? Learn all about the polyhistidine tag, or HIS, in this blog post!

An overview of gRNA modifications available to CRISPR users and some of the advantages of using synthetically modified guides in your experiments.

What are we asking of our audience when we tell them about our science? Cognitive load explains how your learners have to work too.

In this post from our Transferable Skills series, learn how problem solving is a core part of your research training as well as a valuable skill for your future career.

Find tips for working with RNA and simple protocols for extracting RNA without using a kit

Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.