We narrowed to 772 results for: Dos

...Unfortunately, many science graduate programs are still doing an inadequate job of supporting science trainee ...into diverse, successful careers.  We’re not even doing a very good job of providing skills training for...

...opportunities to fall in your lap. Sometimes, in order to do the thing you love, you have to create opportunities...honest), I would tell them to follow your passion. Do what you love. Don't worry about the rest.”- Jason...

...Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using ...each new batch of competent cells, don’t forget to do a negative control (plate your cells without adding...

...double-stranded DNA (dsDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle...cells for rAdV vector production, as HEK293 cells do not contain E4. Download the Bert Vogelstein’s lab...infection, wild-type adenovirus as well as rAdV vectors do not integrate into the host genome. Instead, they...cell line. Recombinant adenoviral vectors typically do not integrate in the host genome and instead are ...for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV...

...Resource Guide Content last reviewed: 23 October 2025 Do you have suggestions for other plasmids that should...

... little more about how I ended up at pgEd, what I do there, and how you can set yourself on a path towards... affiliation, and ethnic or personal identity. We do this through a variety of activities, including creating...newspapers, or starting your own blog. Even if you do not end up pursuing a career in science communication...

...without a kit that outlined several reasons why doing something without a kit has advantages: less plastic...without saying that one should take care whenever doing any type of DNA or RNA purification to avoid contamination...expression. There are several things that researchers can do to combat this issue. First and foremost, be mindful...

... plans to raise salaries. What are institutions doing after the injunction? We are particularly interested... have found out about what their institution is doing from Future of Research over social media, not from...recommendations, even when a federal mandate encourages them to do so. Immediately after the injunction, as we started...

...procedure dramatically reduces the number of clones that do not contain the recombinant plasmid and therefore...are identified much more efficiently, as those that do not contain the desired insert should not grow.   ...be present. Most common cloning strains of E. coli do not contain the F plasmid (and are considered F-)...

...cells - a rich soup of life encircling the tumor. How do microenvironment enzymes and metabolic reprograming...mediators and improving cancer cells proliferation.  How do microenvironments affect tumor spread and drug resistance...for a tumor to spread and to resist drugs. And to do that, let’s take a look at exosomes. Proficient in...

...vector propagation and sequencing a headache. How do I maintain ITR integrity and prevent unwanted mutations... to reduce the risk of plasmid recombination. How do I validate my ITR sequences?  Next-generation sequencing...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes...

...of proteins functioning in a variety of ways. How do scientists focus on the activity or function of a...plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing a polyhistidine...perform assays that they otherwise would not be able to do. We hope that this blog has answered all of your ...

...you have all this knowledge, the question is: How do you know what’s too much (or too little)?  The answer...things aren't working out, adjust the ask.  I used to do a lot of outreach to student groups and being aware...think about everything you’re asking your learners to do, it becomes a lot easier to build successful, accessible...

...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...just a question you have about the world. You might do some background research to better understand it ...method to problems in a variety of contexts.  How do you develop problem solving skills? Thankfully, as...

...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to ...mouse lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can be used...

...technologies and spaces where interested parties can do research. This interest manifested itself in the ... are on the lookout for genome modifications that do not exist in the CGC library but that would be useful..., we have members with a wealth of skills to help do science on the cheap using equipment that would otherwise...

...unlikely you’ll be able to do them all for every western blot assay you run. How do you know which steps to...visualize them with a conjugated antibody. If you do not need such precise measurements, you can use a... double ladder on the lefthand side. Whatever you do, be consistent and document it.   Did it work? Save...directly from tissue or supernatant, and you’re doing the purification and cross-adsorption in lab, consider...adjusting your exposure time first, since you can do that without having to re-run the western.  Remember...the data and what level of accuracy you’ll need to do that confidently.  Once you’ve run your western, ...

...visited Cambridge to spend some time learning and doing research at Novartis. Listen to learn how Kwabena...

...Additional Resources AAV Titers: Where do they come from and what do they mean? AAV: A Versatile Tool for...

... Reddit AMA Here are some concrete things you can do to develop those transferable skills while you are...relies on a division of labor Serve on a committee and do something big like plan a conference Recruit many...