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We narrowed to 772 results for: Dos

Showing: 241 - 260 of 772 results

  1. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...” of mentors as one key to their success. But how do you find these elusive teachers, supporters and advisors...be read or watched during the meeting.  Scientists do better with process, so don’t just get together and...blog in this series will have more ideas on what to do once you are in a mentoring relationship.  Celebrate...

  2. Retrovirus Plasmids

    Type
    Collection
    ... luciferase in mammalian cells Wong Return to Top Do you have suggestions for other plasmids that should...

  6. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using ...each new batch of competent cells, don’t forget to do a negative control (plate your cells without adding...

  7. Adenovirus Guide

    Type
    Guide
    ...double-stranded DNA (dsDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle...cells for rAdV vector production, as HEK293 cells do not contain E4. Download the Bert Vogelstein’s lab...infection, wild-type adenovirus as well as rAdV vectors do not integrate into the host genome. Instead, they...cell line. Recombinant adenoviral vectors typically do not integrate in the host genome and instead are ...for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV...

  8. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...vector propagation and sequencing a headache. How do I maintain ITR integrity and prevent unwanted mutations... to reduce the risk of plasmid recombination. How do I validate my ITR sequences?  Next-generation sequencing...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes...

  9. To Each HIS Own

    Type
    Blog Post
    ...of proteins functioning in a variety of ways. How do scientists focus on the activity or function of a...plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing a polyhistidine...perform assays that they otherwise would not be able to do. We hope that this blog has answered all of your ...

  10. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...just a question you have about the world. You might do some background research to better understand it ...method to problems in a variety of contexts.  How do you develop problem solving skills? Thankfully, as...

  11. Engaging with science and society at pgEd

    Type
    Blog Post
    ... little more about how I ended up at pgEd, what I do there, and how you can set yourself on a path towards... affiliation, and ethnic or personal identity. We do this through a variety of activities, including creating...newspapers, or starting your own blog. Even if you do not end up pursuing a career in science communication...

  12. RNA Extraction Without A Kit

    Type
    Blog Post
    ...without a kit that outlined several reasons why doing something without a kit has advantages: less plastic...without saying that one should take care whenever doing any type of DNA or RNA purification to avoid contamination...expression. There are several things that researchers can do to combat this issue. First and foremost, be mindful...

  13. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...cells - a rich soup of life encircling the tumor. How do microenvironment enzymes and metabolic reprograming...mediators and improving cancer cells proliferation.  How do microenvironments affect tumor spread and drug resistance...for a tumor to spread and to resist drugs. And to do that, let’s take a look at exosomes. Proficient in...

  14. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ... plans to raise salaries. What are institutions doing after the injunction? We are particularly interested... have found out about what their institution is doing from Future of Research over social media, not from...recommendations, even when a federal mandate encourages them to do so. Immediately after the injunction, as we started...

  15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...procedure dramatically reduces the number of clones that do not contain the recombinant plasmid and therefore...are identified much more efficiently, as those that do not contain the desired insert should not grow.   ...be present. Most common cloning strains of E. coli do not contain the F plasmid (and are considered F-)...

  16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce...many suitable dyes to choose from and you can opt to do-it-yourself with a kit or purchase it commercially...but you can still choose what dye to use  What to do once you’ve chosen a gRNA and modification(s) Congratulations...

  17. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...you have all this knowledge, the question is: How do you know what’s too much (or too little)?  The answer...things aren't working out, adjust the ask.  I used to do a lot of outreach to student groups and being aware...think about everything you’re asking your learners to do, it becomes a lot easier to build successful, accessible...

  18. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to ...mouse lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can be used...

  19. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...technologies and spaces where interested parties can do research. This interest manifested itself in the ... are on the lookout for genome modifications that do not exist in the CGC library but that would be useful..., we have members with a wealth of skills to help do science on the cheap using equipment that would otherwise...

  20. Caltech Systemic Capsids

    Type
    Collection
    ...mouse strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used...choose the best capsid for your cell type. First, do you want to target CNS or PNS? Then consider...
Showing: 241 - 260 of 772 results