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We narrowed to 768 results for: Dos

Showing: 241 - 260 of 768 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...we understand what genes do! Viruses have several options to allow you to do exactly that: shRNAs are...clinical promise moving forward. What viruses can do for you Whether you are interested in basic science...long-lasting immune response even after a single dose. Vaccine development strategies. Image courtesy...

  2. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...inducible? How long do you need control? Will temporary control (a few days) be enough, or do you want a permanent...101: Repressible Promoters CRISPR 101: Which Cas9 Do I Choose for my Experiment Additional resources ...

  3. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...phenotype in the surviving population. Selections do require there to be a culture condition that can ...sound like a great shortcut, right? Who would ever do a screen when a selection can get you to the answer...culture condition which eliminates manipulations that do not affect the function of interest. An example where...culture cells in the drug of interest at a lethal dose for non-manipulated cells and then assay cells which...

  4. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...groups and individuals with similar ideas to actually do something about it. Building a diverse team with ...There are many brilliant minds out there, but how do you get them to connect? Through networking of course...solving a community’s issue without involving them can do more harm than good. Check out this webinar for more...

  5. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling too many fragments at once...by methylation like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and ...plasmid, and analyze it using the following steps: Do a single or double digest to be sure that the plasmid...

  6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...need to sort through them to find the desired clone. Do your selection, then screen Why add a screen to your...plasmids that contain the gRNA (white) from those that do not (blue). Let’s take a look at a few screening ... containing plasmids with inserts from those that do not is by using a restriction digest. The key is ...

  7. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...” of mentors as one key to their success. But how do you find these elusive teachers, supporters and advisors...be read or watched during the meeting.  Scientists do better with process, so don’t just get together and...blog in this series will have more ideas on what to do once you are in a mentoring relationship.  Celebrate...

  11. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using ...each new batch of competent cells, don’t forget to do a negative control (plate your cells without adding...

  12. To Each HIS Own

    Type
    Blog Post
    ...of proteins functioning in a variety of ways. How do scientists focus on the activity or function of a...plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing a polyhistidine...perform assays that they otherwise would not be able to do. We hope that this blog has answered all of your ...

  13. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce...many suitable dyes to choose from and you can opt to do-it-yourself with a kit or purchase it commercially...but you can still choose what dye to use  What to do once you’ve chosen a gRNA and modification(s) Congratulations...

  14. RNA Extraction Without A Kit

    Type
    Blog Post
    ...without a kit that outlined several reasons why doing something without a kit has advantages: less plastic...without saying that one should take care whenever doing any type of DNA or RNA purification to avoid contamination...expression. There are several things that researchers can do to combat this issue. First and foremost, be mindful...

  15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...cells - a rich soup of life encircling the tumor. How do microenvironment enzymes and metabolic reprograming...mediators and improving cancer cells proliferation.  How do microenvironments affect tumor spread and drug resistance...for a tumor to spread and to resist drugs. And to do that, let’s take a look at exosomes. Proficient in...

  16. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...just a question you have about the world. You might do some background research to better understand it ...method to problems in a variety of contexts.  How do you develop problem solving skills? Thankfully, as...

  17. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...you have all this knowledge, the question is: How do you know what’s too much (or too little)?  The answer...things aren't working out, adjust the ask.  I used to do a lot of outreach to student groups and being aware...think about everything you’re asking your learners to do, it becomes a lot easier to build successful, accessible...

  18. Engaging with science and society at pgEd

    Type
    Blog Post
    ... little more about how I ended up at pgEd, what I do there, and how you can set yourself on a path towards... affiliation, and ethnic or personal identity. We do this through a variety of activities, including creating...newspapers, or starting your own blog. Even if you do not end up pursuing a career in science communication...

  19. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ... plans to raise salaries. What are institutions doing after the injunction? We are particularly interested... have found out about what their institution is doing from Future of Research over social media, not from...recommendations, even when a federal mandate encourages them to do so. Immediately after the injunction, as we started...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...procedure dramatically reduces the number of clones that do not contain the recombinant plasmid and therefore...are identified much more efficiently, as those that do not contain the desired insert should not grow.   ...be present. Most common cloning strains of E. coli do not contain the F plasmid (and are considered F-)...
Showing: 241 - 260 of 768 results