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We narrowed to 824 results for: EED

Showing: 241 - 260 of 824 results

  1. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... but as a scientist. When our elementary school needed a new coordinator for the annual Math & Science...questions elementary students can ask. It doesn’t just need to be people who think of themselves as “scientists...on Twitter, where I was able to get some great feedback from teachers and other science communicators....grade science teachers with whom I worked, for feedback on my presentation, she said “it was such a valuable...Middle School for their tremendous guidance and feedback. Additional resources on the Addgene blog Browse...

  2. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...applications and needs. The more niche and specific your needs are, the more you may need to pay attention...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your...integration of resistance genes in their genomes. If you need a plasmid that can go between plants and bacteria...

  3. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...the insert. Orientation-specific primers: If you need information about insert orientation, then you might... The more background, the more colonies you will need to screen.   Saving clones for later culture: ... as the template for your PCR reaction. You just need to lyse the bacteria to release the plasmid DNA ... will greatly reduce the number of clones you’ll need to send for sequencing, but won’t tell you if your...sequencing to verify the insert sequence before proceeding with your experiment. Shorter amplicons tend...

  4. Plasmids 101: Protein tags

    Type
    Blog Post
    ...protein tags. Biochemists and molecular biologists who need to overexpress and purify proteins can face any ...are being spent to make something the cell doesn’t need to make. Eukaryotes and some bacteria deploy proteosomes...purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western blots...Frequently, a single tag is not enough. What if you need one tag to increase solubility and one tag for purification...other proteases are available, but scientists would need to incorporate their recognition sites into their...

  5. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...and helpful feedback!  What if I’m not a good writer? That’s okay! Your cover letter needs to be coherent...you are job searching, you may be wondering if you need to go to the effort of writing a cover letter. After...free of typos and grammatical errors. It does not need to be a literary masterpiece. For most jobs, the...make the connection between your skills and the needs of the job. Make it clear that you are interested...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...done at one constant temperature, eliminating the need for thermocyclers. This is followed by in vitro ...-minute incubation  Time: ~90 minutes Equipment needed: 37°C heat block, LED blue light illuminator or... the Cas protein used. Here, all the components needed for the reaction are mixed in a single, one-pot...paper, likely approximately 1-2 hours Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence...Time: Not mentioned, likely ~1-2 hours Equipment needed: PCR machines/Heat blocks, Lateral flow strips,...

  7. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... the fluorescence signal intensity. However, you need to be careful when selecting the primary antibody...cells, and dendritic cells. In this case, we simply need to label the lineage markers of all cell types except...proteins alongside the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure in...   Figure 3: For intracellular labeling cells need to be fixed and permeabilized prior to incubation...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half of the dye...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...practical limitation is sequencing depth and cost. You need at least 100-200 reads per variant, so the more ... one library, the more sequencing reads you will need. See Fowler et al for more details on how to determine... work for the one-pot mutagenesis method. There needs to be a BbvCI site present in the backbone. Does...formed will be sense. Therefore, mutagenic oligos need to be anti-sense so they will anneal to the template...If the sites are in opposite directions, you just need to use a plasmid with BbvCI restriction sites in...

  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...infected cells. To truly cure HIV-1, researchers need to vanquish these hidden viral reservoirs, and CRISPR...off-target effects. In such a therapy, CRISPR/Cas9 would need to 1. excise the HIV-1 genome from every infected...sequence-specific binding. Patients’ HIV-1 genomes will need to be sequenced to determine the optimal gRNAs for...excision approaches, and each of these gRNAs will need to be validated for high on-target binding and low...evolve resistance to CRISPR therapies through PAM or seed sequence mutations. Multiple gRNAs could be used...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...low-efficiency process, and usually involves the need for single cell cloning and subsequent screening...consuming process and should not be undertaken lightly! Indeed, truly achieving the gold standard requires not...early days for this technology, and the user may need to optimize numerous parameters to generate the ... but this can decrease the number of guides one needs to test in the lab. Importantly, for any modification...Conclusions Selection of gRNAs for an experiment needs to balance maximizing on-target activity while minimizing...

  11. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...member first author because they “needed it more”. My friend didn’t heed this warning and profoundly regretted...interaction (and I did). Some of the information you will need is about you. It’s worth taking time to honestly...relationships can help identify your priorities and needs, but sometimes it just takes introspective thought...8 children and used to getting the attention you need? Are you good at asking for help? If you are good...

  12. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...Nature found a way to re-create a functional virus. Indeed, by testing several vectors and packaging cell ...promoter has been inserted in this LTR so you don’t need Tat to transcribe the viral genome in producing ...inducibly. If your gene of interest is toxic or needs to be expressed a certain point in the cell cycle...viral particles themselves. For this you will first need to transfect producing cells, usually 293T cells...you are transducing non-adherent cells you might need to centrifuge your cells with the virus in order...

  13. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    ... lab where they can learn the plus skills needed to succeed on the career path of their choice. This failing...pandemic has demonstrated that, more than ever, we need all of our science trainees to be successful after..., the ASBMB and Burroughs Wellcome Fund provided seed funding to take this initiative to the next level...

  14. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...tag-specific antibody (Jarvik and Telmer 1998). Indeed, this method can be used at high throughput because... each cell type, the transfection/delivery step needs to be optimized to achieve high knock-in efficiency...molecules can be directly purchased and there is no need to construct plasmid vectors or synthesize/purify...lab is new to CRISPR/Cas9 experiments, there is no need to worry at all. Setting up tag knock-in experiments...most crucial aspect of knock-in experiments. You’ll need to optimize csRNP delivery for each cell type you...

  15. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...Confocal Microscopy (Spinning Disk) increases the speed at which a confocal image can be acquired. Assembling...energy of blue light, two photons of red light are needed to excite GFP compared to only one of blue. Only...temporal resolution on the millisecond scale may be needed. Most commonly these experiments are performed ...lack of a requirement for high temporal imaging speeds. Standard fluorescence widefield microscopy can...proper sampling. Therefore, large, cleared tissues need to be imaged via Lightsheet microscopy. Although...

  16. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...remember that we, scientists and non-scientists alike, need to stay active and involved if we wish to make positive...access to full reproductive health care. And it’s needed most in low-income and minority communities, where...restrictions that conflict with scientific evidence. We need representatives and policymakers who not only believe...children to learn little things about science. We need to keep encouraging people to look for evidence ...criticisms of what I saw are that we, as a society, need to: 1) use more citations for out data so our sources...

  17. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...literature search to see what others have done, you’ll need to experimentally test your chosen capsid before...a few additional, specific considerations you’ll need to be aware of before starting an experiment. Controlling...are only starting recommendations; you will still need to experimentally determine the best titer for your... If you think you’ve found a good fit (or if you need more options) we recommend you check out Caltech.... K., Monet, D., Ngo, V., Ding, X., Omstead, V., Weed, N., … Gradinaru, V. (2023). Functional gene delivery...C. L., Balazs, A. B., Daneman, R., Bloom, J. M., Seed, C., & Deverman, B. E. (2019). Delivering genes ... A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V....

  18. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...Plasmids of course! You just need three plasmids to start making the AAVs you need for your experiment:  the...adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid...regions of dsDNA. To generate a 3’ hydroxyl group needed for DNA replication, Rep78/68 binds the Rep binding...

  19. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...discarded.  Seeding Labs does an amazing job shuttling unwanted lab supplies to places that really need them....resourced areas of the world.  Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student...s, my lab was well funded and we didn’t feel the need to reuse plasticware or think about conserving reagents...

  20. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...when you wantYou can just turn them offNo need for any washNo need for any wash…” 🎶- Barbara Di VenturaIf... AraC, it still reached the level of expression needed for most applications.   Figure 1: With ...promoter, can be controlled with light without the need of any cloning.     What advantages does BLADE...
Showing: 241 - 260 of 824 results