We narrowed to 642 results for: EMB

...figure above), which generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm...nucleofection, which forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques...protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods, while...

...compromising the photon budget. Likewise, the switch from ensemble imaging to single-molecule imaging places a greater...recently discovered that incorporation of four-membered azetidine rings could substantially enhance the...analogs without sacrificing their small size and membrane permeability (Figure 2c). These cell-permeable...

...data from the ENCODE project, find enhancers in embryonic stem cells, and conclude with a targeted CRISPR...for NANOG, an essential transcription factor in embryonic stem cells (ESCs). Your ChIP-seq finds just over...CRISPR screen. We start by infecting millions of embryonic stem cells (ESCs) with a lentiviral vector, an...

... The G·U wobble base pair. EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001 Additional resources...mammalian cells. This was the first codon optimized membrane protein expressed in mammalian cells. In this ...

...for attendees and helping societies help their members get the most out of their meetings. Amanda Gunn...Gunn ran randomized coffee trials for us at ASCB|EMBO 2019, matching Mentors with Singletons for short coffee... a culture that brings more value to ALL their members.  Shoutout to our mentors and supporters Thanks...

...development, complex genetic programs specify and assemble diverse arrays of neurons, forming the neuronal...subgenome-scale CRISPRa screening approach in embryonic stem (ES) cells to determine which transcription...gene-function relationships in the CNS using CRISPRa as a member of the Gelbard Laboratory at URMC.     References...

...structures in RNA transcripts Effects on transcription (remember, it’s not all about translation - e.g. codon choice... isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157...transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366. PubMed PMID: 2265611...

...plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy- and light-chain DNAs into a...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

...Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using...predict high accuracy junction sets for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet...Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene constructs...

...capsid (Cap) genes necessary for the production and assembly of viral capsids. The capsid protein encoded in...proteins necessary for AAV vector production and assembly. The capsid protein encoded in the packaging plasmid...SYBR Green Technology Content last reviewed: 10 September 2025 Do you have suggestions for other plasmids...

... streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher linkers and ...and target capture module (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP nucleosomes...loss. Ci Ji Lim’s lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that...

...devised a “Modular fluorescence unit fused with TRansmembrane region-to-IntrAcellular loop linkers” (MTRIA...they observe fluorescence localizes to the plasma membrane only when the RTK is activated and the ITAM phosphorylated...relies on a change in localization to the cell membrane and not a direct change in the fluorescent properties...

...heads and long legs that protrude from the cell membrane, physically connecting the extracellular environment...the integrin, where it is resisted by the ligand embedded in the extracellular environment. This further...the extended open integrin conformation. During embryonic development, extended-open integrins enable tissue...

...hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly...split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of a “Top” and ...fluorescent split aptamer for visualizing RNA–RNA assembly in vivo." ACS synthetic biology 6.9 (2017): 1710...

...proteins, which are directly trafficked to cell membranes. Membrane trafficking of farnesylated derivatives enables...fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without Cre recombination, RFP...

...deposited versions with both PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most... modulators of cell signaling pathways during embryonic development and cancer. But there is still much...intestinal peptide (VIP), or lysosomal-associated membrane protein 5 (LAMP5) — as well as cholinergic (choline...

...optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters could not be used ...Venus fluorescent protein in conjunction with a membrane-inserted voltage sensing domain (LOTUS V; Luminescent...Universal Sensing of Voltage). With this sensor, membrane depolarization increases the BRET signal whereas...

... scientific mentor and choosing the right lab. Remember that your time is valuable! Don’t jump on the ...to plan out a very distinct and certain path, embracing uncertainty can help you both in your career and....org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 . https://doi....

...committee in graduate school. Thesis committee members provide expertise in designing and interpreting...to introduce yourself to and interact with the members of your field, and present your research. Face ...family. Those people are your support network, so remember to thank them verbally and often for their love...

...Neurodegeneration Research Collection Addgene has assembled the following to help you find information and...cases having at least one other affected family member. These familial cases, usually inherited in an ...ER contact with other organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein...