...CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems. The original pCas... that same year. Her research is focused on the escape mechanism of genome editing in microorganisms which...Yang J, Yang S (2015) Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl ...system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https...

...LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID, where two proximity labeling enzymes...slow-growing protein fiber in the cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably ...different promoters, specific time points appear as fluorescent bands in the fiber, resembling a “ticker tape... treated with a phorbol ester activator gave fluorescent signal in the ticker tape from cFos::GFP-iPAK4...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre....) are generated from lentiviral preparations (described above) that are subject to a concentration step...10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of Lentiviral Infectious...

...are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used to study...without signs of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin...mammalian cells. Science 365, 1469–1475 (2019). Maresca, D. et al. Nonlinear ultrasound imaging of nanoscale...

...generally not true. Because of the variations in fluorescent signals, and how the machine reads/interprets...light that “scatters” past the cell, instead of fluorescent markers. There are various types of SSC and FSC...which is comprised of antibodies conjugated to fluorescent markers and/or cellular dyes and stains. These...anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers are used to identify monocytes (upper...

...insert into RNA. In practice, the term "promoter" describes the combination of the promoter (RNA polymerase...   Primarily used for RNA     transcript     Description Expression Additional considerations CMV General...promoters     Promoter     Primarily used for Description Expression Additional considerations T7 in ...identify the right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max...

...Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase." Journal of bacteriology 173.16...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013..."Optimization of a ligase detection reaction-fluorescent microsphere assay for characterization of resistance-mediating...

... I thought I could see GFP expression with a fluorescence microscope, but the GFP expression was so low...Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide libraries ...was a gift from John Doench & David Root and is described in Rational design of highly active sgRNAs for...were gifts from John Doench & David Root and are described in Optimized sgRNA design to maximize activity...

...update, we have added new sections to share and describe some incredible new CRISPR technologies developed...much-anticipated prime editing section and additions describing CRISPR’s ability to make large genome edits using...transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements...

...Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous...mCyRFP1 - a new, monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot...: mScarlet - a new, bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet ...

...challenge. Chromoproteins are a subset of the fluorescent protein family that give off color in ambient...the Addgene blog Read our other blog posts on fluorescent proteins Check out educational resources on the... the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...

...Medicine have also recently described how the glycoprotein transforms itself to escape vesicles within host ...It’s pretty hard to escape news and fears of Ebola these days, as the infectious disease once limited...

... interested in our fluorescent protein background. Download the orange fluorescent protein background....If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET ...

...fluorogenic primers Low Single self-quenching fluorescent molecule connected to a primer  No Yes ...normalize for that. Passive reference dyes are inert fluorescent dyes that are not dependent on amplification ... It is possible to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to ... Tip🔥 The ABI machines automatically record fluorescent data on all channels - helpful if you forget ...

... discusses some of the programs SITN runs and describes some of the more popular topics from the Science...telling. Chapter 5: The SITN Blog - Vini and Amy describe how the SITN blog is a source of scientific information...underwater? Chapter 8: Work-Life Balance - Vini describes how she balances working in the lab and working...

...citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines recombineering...plasmid tools that can be used in this way. As described in the above CRISPRi section, the Marraffini Lab... aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae. These vectors contain...system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https...

...plasmid with its elements described below.   Vector Element Description Origin of Replication ...quantity inside a cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor...

...Each trap uses a slightly different approach (described in detail below), but fundamentally the reporter...of interest in any way. For example, if green fluorescent protein (GFP) was the reporter gene and green...construct can be something phenotypic (such as a fluorescent protein or something species-specific) or a selectable...

...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...responsibilities of a role, so it helps to see how people describe their actual work. To do this, try looking at ...

...fly fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome... Mehmet Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease...also got to show off our new CRISPR page (which describes how to plan your CRISPR experiment for our new...