We narrowed to 622 results for: Erf

...cultured following a differentiation protocol before performing single-cell RNA sequencing. The sequencing results...were differentiated and single cell RNA-seq was performed to map TFs to cell fates. (Adapted from Joung ...

...knowledge, but the sensor can also kinetically outperform current methods. Unger plans to use the sensor...and temperature and leaf autofluorescence can interfere with microscopy. Thus, Bernach sought to develop...

...the gradual change in pH visible at the sample interface upon thaw (Fig. 2). Before thawing, all the samples...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

...lentiviral vectors from the repository for you. We perform test infections with all of our viral preps and.... We provide enough lentivirus for you to both perform an optimization-scale infection and at least one...

...BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig...Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments for discovering novel...

...from E. coli. This tool should serve as both a powerful tool for generating complex regulatory systems...electrical information. Such voltage imaging can be performed in intact animals and offers many advantages over...

... editing experiments in this publication were performed using a technique called nucleofection, which ...found on our plasmid pages. We encourage any labs performing genome editing experiments to try nucleofection...

...blue and emission spectra are red. Neither is a perfect match to the filter set but the excitation filter...reported brightness measurements. Here, EGFP outperforms the improved folding variants, presumably due...

...efficient selection. This is usually achieved by performing a "kill curve" (basically growing cells in various...Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids 101: Antibiotic...

...variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was cloned into a ...frequency within functional light chain sequences. By performing a BciVI digest of the PCR products prior to cloning...

...novel or significant findings, and combined with powerful cognitive biases such as motivated reasoning and...process of sampling and inference-making. Even perfect transparent and rigorous studies will give false...

...quite different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell... strand to yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur...

...access neurons, and study their connections, in a powerful way. Using brain structure to understand brain...exhibit retrograde transport and can therefore perform retrograde tracing, but often at the cost of toxicity...

...underutilized team potential. I often thought that performing a cell culture experiment myself would take less... from technical work. On the other hand, it’s perfectly valid to decide that managing isn’t for you at...

...While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9 nuclease, which...generating a monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top ...

...intense student engagement and joy, rather than a perfect lesson plan or outstanding test scores.  As a result...Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro, and the...

...asked to draft a personal statement, which is the perfect opportunity to paint a comprehensive picture of...technology, background, and lighting for a smooth performance. Most importantly, make certain that everyone...

...can occur when Cas9 cleaves a site that is not a perfect match to the guide RNA (gRNA) used to target the...and variants to choose from. While no enzyme is perfect for all experimental approaches, knowledge of both...

...occurs prior to ligation as incompatible DNA ends interfere with that step. Accordingly, NHEJ has a vast toolbox...from the broken end after cleavage, which may interfere with loading of Ku and normal NHEJ activity. Other...

... idea is solid, and “the pitch is packaged to perfection”, seed money will hopefully be raised and the...Billion Dollar Molecule: One Company's Quest for the Perfect Drug by Barry Werth Ten Tips for Raising Startup...