We narrowed to 365 results for: FUS
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TypeBlog Post... vector type Any tags, selectable markers, and fusion proteins listed for each plasmid If the plasmids...
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CRISPR in the Clinic
TypeBlog Post...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...activated through administered drugs that must diffuse throughout the body, the onset of receptor activation... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post... In addition to generating fluorescent protein fusions, plasmids are available for tagging with epitope... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post... McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...heterogeneous cultures. HeLa cells expressed a fusion of keratin with mGold (top) and mVenus (bottom)... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...complicated. These strains are more picky eaters and refuse to eat liver paste mixed with E. coli. Thus, to... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...spatial control of CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post... a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime... -
PhD Applications After COVID
TypeBlog Post...funded? Are there any financial aspects that are confusing? During a traditional campus visit, many students... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources... -
3D Printing Meets CRISPR Cas9
TypeBlog Post... initial model of this protein – but were very confused because the HNH nuclease active site wasn’t even... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post...your elevator pitch where the other person seemed confused, bored, or particularly excited about? If you ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...products into E. coli. Ligases within the E. coli then fuse together the PCR products via overhangs left over... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...to the gene of interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post... fly stocks and other reagents. If you’re ever confused about the best way to study YFG in flies, there...