We narrowed to 319 results for: INO

1. Hot Plasmids and Viral Preps - March 2021

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   ...fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins...

2. Progress Towards a PAM-Free CRISPR

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   ...knock-in a patient mutation or need to make a precise amino acid change, there is very little flexibility in...

3. Hot Plasmids February 2024

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   ...the epitope tags we know and love, the FLAG tag (amino acid sequence DYKDDDDK) may be one of the most versatile...

4. 6 Tips for Grant Writing

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   ...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...

5. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...was not possible, but one of our members took an Arduino microcontroller and a motor controller and made...

6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...

7. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...viral production. This recombination produces a minor pre-recombined population that does not require ...

8. Truncated gRNAs for Regulating Gene Expression

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   ...additional effector domains (4). Taken together, these minor gRNA alterations can be exploited to quickly and...

9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ...different trinucleotides (corresponding to different amino acids) in the regions that bind to the antigen. ...

10. Viral Vectors 101: Producing Your rAAV

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    ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

11. Tips for Using BLAST to Verify Plasmids

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    ...blastx results start the alignment at a more distal amino acid. We consistently run our blastx searches with...

12. Tetracycline Inducible Expression

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    ...et al. used random mutagenesis to identify which amino acid residues of tetR were important for tetracycline-dependent...

13. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

14. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ... Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino for their helpful discussion of this book....

15. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...https://doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to...

16. Antibodies 101: Producing Recombinant Antibodies

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    ... though this can vary depending on the specific amino acid sequence (Maity et al., 2015). If your antibody...

17. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ... (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure...

18. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...histone proteins K4 and K27 – locations of the amino acid lysine in H3 me1, me3, and ac – denoting ...

19. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, C., Ellerbrok, H., Kamuzinzi, ...

20. Cpf1: A New Tool for CRISPR Genome Editing

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    ...two nucleases, AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered...