We narrowed to 327 results for: INO

1. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   ...end positions without changing the order of the amino acids in the protein) may be able to boost FRET ...

2. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   ... which terminus, N or C, to tag and which short amino acid linker to use. These decisions were made based...

3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   Blog Post

   ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

4. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   ...Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez, C.P., Keleş, Ö., Kusnyerik, A., Azoulay...

5. Hot Plasmids and Viral Preps - March 2021

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   Blog Post

   ...fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins...

6. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

   ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...

7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   Blog Post

   ... editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar ...

8. Progress Towards a PAM-Free CRISPR

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   Blog Post

   ...knock-in a patient mutation or need to make a precise amino acid change, there is very little flexibility in...

9. Hot Plasmids February 2024

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   Blog Post

   ...the epitope tags we know and love, the FLAG tag (amino acid sequence DYKDDDDK) may be one of the most versatile...

10. 6 Tips for Grant Writing

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    Blog Post

    ...other scientists that we forget that some of the terminology we use routinely isn’t common to non-scientists...

11. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...was not possible, but one of our members took an Arduino microcontroller and a motor controller and made...

12. Plasmids for Stem Cell Research

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    Collection

    ... reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol...

13. Tetracycline Inducible Expression

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    Collection

    ... et al. used random mutagenesis to identify the amino acid residues of TetR that were important for tetracycline-dependent...

14. Hot Plasmids: Summer 2025

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    Blog Post

    ...small enough to be carried by viral vectors (~400 amino acids, less than a third of the size of Cas9). ...

15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...

16. Viral Vectors 101: Producing Your rAAV

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    Blog Post

    ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

17. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post

    ...viral production. This recombination produces a minor pre-recombined population that does not require ...

18. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    ...additional effector domains (4). Taken together, these minor gRNA alterations can be exploited to quickly and...

19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    ...different trinucleotides (corresponding to different amino acids) in the regions that bind to the antigen. ...

20. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    ...blastx results start the alignment at a more distal amino acid. We consistently run our blastx searches with...