We narrowed to 527 results for: MEA

...secondary antibodies are generally polyclonal, which means that multiple secondary molecules can bind to a ...target and sample type. Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking...

...are that it does not use ubiquitous promoters, meaning that not all cell types can be labeled, and that...labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage will express the...

...proteins can be accurately determined in vitro by measuring the fluorescence intensity of purified protein..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3...

... be aware of deadlines and think about what they mean in terms of the utility of your tasks. In my cell...accomplish these goals. Practically speaking, this means I write everything in a Word or google doc that ...

...DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the different...nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move from sequencing ...

...β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose cannot be taken up by cell...common lab strains; however, these tables are by no means exhaustive! For a more comprehensive list and additional...

...neurons to label surface proteins with a membrane-impermeable biotin substrate (Figure 1). Biotinylated proteins...topology of FRCaMP (itself based on mApple, which means it likely has the blue-light photoswitching issue...

...then prevents transcription from occurring, which means that transcription is now OFF.   We’ll look at ... 3 binary systems listed above are orthogonal, meaning that they do not affect each other. For example...

... western. To prepare the samples for SDS-PAGE, measure the protein content and normalize to ensure equivalent...gauge protein abundance they provide qualitative measurements. If you require precise quantification of a ...

... study to be “reproducible” does not necessarily mean that someone has replicated it precisely. You can...affirming to have a hypothesis confirmed, it also means that you’re more likely to get a publication (for...

... the clinic. At the bench, improving antibodies means improving research. More specific antibodies support...display are common. ELISA enables quantitative measurement of antigen-antibody interactions in a plate-based...

...target within ~30 nt of your proposed edit, which means there are so few gRNAs to choose from that sequence...of the repair template (Yang et al., 2013). This means that, for gene editing, there are usually few potential...

...FRET can be measured by exciting the most blue-shifted fluorescent protein (donor) and measuring the resultant...

...products can look smeared, so having an undigested control can help distinguish between a smear and a faint ...

...and click measure, be sure to record the concentration and purity. Note: Purity is measured under the ...specific wavelength that they maximally absorb. By measuring the absorbance of a liquid you can accurately ...substance in that liquid. In order to accurately measure the concentration of a substance based on its absorbance...specific to your lab. Spectrophotometer Tips Before measuring any samples, be sure to ‘blank’ the spectrophotometer...resuspended in, but with no DNA added. 'Blanking' measures the background inherent to the machine and your...your solvent. If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal...

...mechanisms of action and other analyses. This also means that between two types, the Cas effector may have...nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu, F. E., Moeller, L., Kocalar...

...microscope before it reaches the detector. This means that two or more objects lying within the diffraction...axial dimension that will appear in focus). This means that the images will appear sharp without interference...

...and inactivates Dronpa is not ideal, since this means Dronpa is quickly inactivated and less photons are...fluorescence the better. More fluorescence usually means more photons are being emitted, making it easier...

... etc. (more on prep sizes below). But what do we mean by DNA quality? There are a few different issues...?  Obtaining higher quantities of DNA generally means starting with higher volumes of bacterial culture...

...expressed from its own promoter. But that doesn’t mean you have to clone and transfect multiple promoter-gRNA...gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler sequences...