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We narrowed to 480 results for: Mag

Showing: 241 - 260 of 480 results
  1. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...Cell Collection at Coriell. More information and images generated from these cell lines can be found in...fluorescent proteins as described here enables live cell imaging to study cellular structures and processes and ...
  2. PCR Applications for Copy Number Variation Assays

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    Blog Post
    ...fluorescent signaling in the presence of dsDNA. Image courtesy of: Ygonaar    Taqman is more sensitive...thousands of PCR reactions using Taqman chemistry (see image, left, of droplet immersion technique, courtesy ...
  3. Transferable Skills: Negotiation

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    Blog Post
    ...confocal microscope. You’re trying to get better images for a paper and she’s trying to get some data for...manuscript is more important to you than collecting your images this week. Even though three examples depend on...
  4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post
    ... Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with permission.   ...recipient bacteria with an integrated copy of CRISPRi. Image from Peters et al., 2019 with permission.    ...
  5. Plasmids 101: Degron Tags

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    Blog Post
    ...which will bind and light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting... avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag could pay...
  6. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol. Rev. 90:1103...et. al. (2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear ...
  7. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post
    ...   Figure 2: Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license...   Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts...
  8. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post
    ...fluorescent label, which can reduce the potential image resolution of super-resolution fluorescence microscopy...Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex...
  9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post
    ...knock in, base edit, activate, repress, and even image your favorite gene. With so many Cas9 reagents available...target strand based on the sequence of the pegRNA. Image from David Liu.   Activation and repression...
  10. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...with mRNA and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s... codons are under expressed compared to others. Image from Hiss et al., 2017. Most commonly, scientists...
  11. Plasmids 101: Shuttle Vectors

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    Blog Post
    ...I fell in love with biology because of an image that was honestly quite boring. My Bio 101 professor ...sorting or to visually select cells in a microscopy image for individual analysis. A shuttle vector designed...
  12. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ... appear darker than full vector particles. This image shows that the vast majority of the vectors consist...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie...
  13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ...groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating ...fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. In short, the Cas13a molecule brings a much anticipated...
  14. Tips for Writing a Good Cover Letter

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    Blog Post
    ...would help you do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills...answer to that is what goes into your cover letter. Imagine you’re applying to a job with a lot of file management...
  15. Tips for Getting a Faculty Position

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    Blog Post
    ...schools. I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...
Showing: 241 - 260 of 480 results