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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 768 results for: Ncl

Showing: 241 - 260 of 768 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...series of single- and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and ...detect action potentials and subthreshold events, including miniature excitatory postsynaptic potentials (...triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

  2. Common Injection Routes in Mice

    Type
    Blog Post
    ... mice. While not an exhaustive list, these can include what’s being injected, the type of experiment, ...method. Many different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses.... the name suggests, in the muscle. Common uses include administering vaccines, pain meds, or other drugs...

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...NgAgo optimization before we can make any major conclusions: That said, many people are having difficulty...Some great places to get your results out there include: Google Groups - I've specifically set up a forum...Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing methods in brain cells...

  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...mind that access and availability to resources (including the internet!) can vary depending on factors like...your best to understand what kind of resources - including your time - will be required to produce an outcome...naked eye or to make things more accessible by including, highlighting, or omitting details. Digital or...

  7. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014;...promote professional success (many of my students include their in-class research experience on their resumes...experiences can make scientific research more inclusive. CBE Life Sciences Education. 13(4):602-606. Brian...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...current genome engineering applications of CRISPR including: generating single or multi-gene knockouts in ...) that harbors two surface exposed cysteines, including the native C547, allowing for ligation to pyridyl...Senior Scientist at Addgene.  Andrew's interests include genome engineering and microbiology.     References...

  9. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ... Selecting crispr in Guides will return links including our CRISPR guide.  For a closer look at this new... deposit prior to publication and were able to include specific plasmid ID numbers. If you know the ID...suggest direct links to other types of searches including depositing labs and institutions. For example,...

  10. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce...CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: lentiCRISPR... observed in small trials for other diseases, including the common hematopoietic disorders sickle cell...

  11. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... and management process or the whole process, including grant readiness, funding opportunity research,...grant training, mentoring and coaching services, including Smartegrants, to help those entering the Grants...LLC, a grant professional development firm that includes the social media community GrantChat. She has ...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...scientists employ GFP for a wide number of functions, including: tagging genes for elucidating their expression...detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations and pH, using...selection: Expression constructs like plasmids often include GFP as a marker to help identify which cells have...

  13. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance ...can be used for an array of detectable proteins including fluorescent proteins, antibiotic resistance, and...

  14. Bioinformatics at Addgene

    Type
    Blog Post
    ... bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose of a data...workflow management system for scientific pipelines, including bioinformatics. We loved the parallels between...language.  The modern architecture of Nextflow included built-in support for containers, such as Docker...

  15. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. In molecular...measurable cell toxicity – using Cas7-11. This included tests done in cell lines known to be more sensitive... — the ability to now make any small changes, including base edits, insertions, and deletions. But CRISPR...

  16. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...spacers of the crRNA can be customized as well, including concatenations into crRNA arrays targeting multiple...by sacB counterselection.  The MUCICAT toolkit includes versions of plasmids with different induction ...concentration. Image from the Yang lab.   In conclusion, the MUCICAT system has the following advantages...

  17. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...the CRISPR workflow. For a multiplex sample, ICE includes visual representations of all detected edit types...indel.   For a complete guide to ICE analysis, including detailed explanation and examples, read the paper...stack Genome Engineering Solutions, which also includes our CRISPR knockout guide design tool, guide validation...

  18. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...rather late stages (i.e. postdoc level) and can include the pressure to publish in high-impact journals...for insights from my time in technical support including tips, advantages, and disadvantages of this overall...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...

  19. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...If you choose this strategy, it’s important to include the proper protein expression elements (ribosome...entry clones available for many popular genes, including Hs.KRAS4a. Use our website to search for your ...Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression Systems...
Showing: 241 - 260 of 768 results