... . https://doi.org/10.1534/genetics.114.169730 Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C...

...did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health...

...science ultimately galvanized scientists to rally in Ottawa to protest the “death of evidence,” and to speak...

...References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for selective...

... 25998715. PubMed Central PMCID: PMC4491443. 5. Scott, Andrew M., Jedd D. Wolchok, and Lloyd J. Old. "...

...population is selected and analyzed further by plotting CD3 versus CD19 to separate B cells, T cells and...

...near a local maximum of PE's excitation spectrum (dotted line, Figure 2). The yellow/green laser, however...

...Capecchi, G., Visaggio, D., Gasperi, T., Parisi, M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (...

...details, check out our article The Basics of Western Blotting.    Figure 4: Western blot showing GFP expression...

...they also continually dose the sample from top to bottom with excitation light which can lead to photobleaching...

...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...

... Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

...represents the target in your sample of interest (bottom). The approach maps onto the five pillars, with...

...Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

...genes are run and the difference in CT values is plotted against the log input concentration for each dilution...

...conversion directly catalyzed by the base editor. Dotted red arrows indicate the change introduced to the...

...Ankyrin-B Human Mouse IgG2a 177495 Anti-Ankyrin-G (blotting) [N106/20R] Ankyrin-G Human Mouse IgG2a 177496...

... faster and less labor intensive than northern blotting. To improve folding of the Broccoli apatamer, ...

...appropriately sized bottle for autoclaving. We make 400 mL of agar in 1 L bottles and 200 mL of agar in...to the same bottle and swirl to form a medium/agar colloid. Cover the opening of the bottle with its cap...Use lab tape to label the bottle with your initials, the date, and the bottle contents. This will clear... in 500 mL bottles. The extra empty volume is necessary to prevent your molten agar from boiling over ...but do not make an air-tight seal!) and tape the bottle with autoclave tape. The autoclave tape will darken...clear up any confusion later if your forget your bottle in the autoclave. Place the gel mix in the autoclave...pouring your plates - be sure to leave room for your bottle of molten gel mix, a tube rack containing the appropriate...

...a sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated... mM MgCl 2 Add the following to the 2 L sterile bottle: 1836 mL deionized water + 100 mL of 1 M Tris HCl...Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and mix by inverting multiple times Adjust pH to...complete media, then transfer the cells into a sterile bottle. Rinse the CS2 with 100 mL of DMEM complete medium...plasmid DNA into the bottle containing the Opti-MEM. Mix well. Add 4 mL of PEI (1:2 μg DNA to μg PEI ratio...ratio). Shake the bottle up/down vigorously for 30 sec (it’s okay to make bubbles). Incubate at RT for 15...