We narrowed to 290 results for: Ott;

1. A Guide to Getting Started in Undergrad Research

   Type

   Blog Post

   ...interests at heart. A great piece of advice I’ve gotten is that not all advice is good advice, and it is...

2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

   Type

   Blog Post

   ... . https://doi.org/10.1534/genetics.114.169730 Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C...

3. Quick Guide to All Things Lentivirus

   Type

   Blog Post

   ...difficult to interpret your results. This can be a bottleneck for your experiment. Single vector systems containing...

4. Antibody Guide

   Type

   Guide

   ...resulting light emissions. In FACS, cells are then slotted in a specific direction based on the emission(s...

5. Antibodies 101: Designing Your First Flow Panel

   Type

   Blog Post

   ...near a local maximum of PE's excitation spectrum (dotted line, Figure 2). The yellow/green laser, however...

6. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

   Type

   Blog Post

   ...Capecchi, G., Visaggio, D., Gasperi, T., Parisi, M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (...

7. Antibodies 101: Flow Cytometry

   Type

   Blog Post

   ...population is selected and analyzed further by plotting CD3 versus CD19 to separate B cells, T cells and...

8. Antibodies 101: Introduction to Antibodies

   Type

   Blog Post

   ...details, check out our article The Basics of Western Blotting.    Figure 4: Western blot showing GFP expression...

9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

   Type

   Blog Post

   ...References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for selective...

10. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    ... 25998715. PubMed Central PMCID: PMC4491443. 5. Scott, Andrew M., Jedd D. Wolchok, and Lloyd J. Old. "...

11. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    ...science ultimately galvanized scientists to rally in Ottawa to protest the “death of evidence,” and to speak...

12. Which Fluorescence Microscopy Technique is Best for Me?

    Type

    Blog Post

    ...they also continually dose the sample from top to bottom with excitation light which can lead to photobleaching...

13. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ... Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

14. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    ...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...

15. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    ...represents the target in your sample of interest (bottom). The approach maps onto the five pillars, with...

16. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    ...Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

17. Deep Dive: qPCR

    Type

    Blog Post

    ...genes are run and the difference in CT values is plotted against the log input concentration for each dilution...

18. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    ...conversion directly catalyzed by the base editor. Dotted red arrows indicate the change introduced to the...

19. Trimmer Lab NeuroMab Collection

    Type

    Collection

    ...Ankyrin-B Human Mouse IgG2a 177495 Anti-Ankyrin-G (blotting) [N106/20R] Ankyrin-G Human Mouse IgG2a 177496...

20. 28 Hot Plasmid Technologies from 2015

    Type

    Blog Post

    ... faster and less labor intensive than northern blotting. To improve folding of the Broccoli apatamer, ...