...population is selected and analyzed further by plotting CD3 versus CD19 to separate B cells, T cells and...

...details, check out our article The Basics of Western Blotting.    Figure 4: Western blot showing GFP expression...

...References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for selective...

... 25998715. PubMed Central PMCID: PMC4491443. 5. Scott, Andrew M., Jedd D. Wolchok, and Lloyd J. Old. "...

...near a local maximum of PE's excitation spectrum (dotted line, Figure 2). The yellow/green laser, however...

...Capecchi, G., Visaggio, D., Gasperi, T., Parisi, M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (...

...science ultimately galvanized scientists to rally in Ottawa to protest the “death of evidence,” and to speak...

...they also continually dose the sample from top to bottom with excitation light which can lead to photobleaching...

...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...

... Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

...represents the target in your sample of interest (bottom). The approach maps onto the five pillars, with...

...Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

...genes are run and the difference in CT values is plotted against the log input concentration for each dilution...

...conversion directly catalyzed by the base editor. Dotted red arrows indicate the change introduced to the...

...Ankyrin-B Human Mouse IgG2a 177495 Anti-Ankyrin-G (blotting) [N106/20R] Ankyrin-G Human Mouse IgG2a 177496...

... faster and less labor intensive than northern blotting. To improve folding of the Broccoli apatamer, ...

...appropriately sized bottle for autoclaving. We make 400 mL of agar in 1 L bottles and 200 mL of agar in...to the same bottle and swirl to form a medium/agar colloid. Cover the opening of the bottle with its cap...Use lab tape to label the bottle with your initials, the date, and the bottle contents. This will clear... in 500 mL bottles. The extra empty volume is necessary to prevent your molten agar from boiling over ...but do not make an air-tight seal!) and tape the bottle with autoclave tape. The autoclave tape will darken...clear up any confusion later if your forget your bottle in the autoclave. Place the gel mix in the autoclave...pouring your plates - be sure to leave room for your bottle of molten gel mix, a tube rack containing the appropriate...

...a sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated... mM MgCl 2 Add the following to the 2 L sterile bottle: 1836 mL deionized water + 100 mL of 1 M Tris HCl...Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and mix by inverting multiple times Adjust pH to...complete media, then transfer the cells into a sterile bottle. Rinse the CS2 with 100 mL of DMEM complete medium...plasmid DNA into the bottle containing the Opti-MEM. Mix well. Add 4 mL of PEI (1:2 μg DNA to μg PEI ratio...ratio). Shake the bottle up/down vigorously for 30 sec (it’s okay to make bubbles). Incubate at RT for 15...

...separator. Keep the Bottom Stack in the plastic tray. Place the Bottom Stack on the blotting surface. Align...lysate, running a precast SDS-PAGE gel, and immunoblotting. Protocols...lysate, running a precast SDS-PAGE gel, and immunoblotting. Sharing speeds science. We believe that sharing... gels. Use lower percentage acrylamide when immunoblotting high molecular weight proteins and higher percentage... percentage acrylamide when immunoblotting low molecular weight proteins. This protocol uses a dry transfer...instructional video to learn how to use western blotting to visualize a protein from cells or tissue samples...Align the electrical contacts on the blotting surface of the iBlot 2 Gel Transfer Device. Wet the pre-run...

...water bath weights can hold bottles in place. After putting your tubes or bottles in the water bath, place...baths that exist as scientists work with tubes and bottles of different sizes in the lab. Some water baths...water baths hold many liters of water to incubate bottles and containers. Water baths can also be placed ...be used in water baths with instructions on the bottle, for example, number of drops per liter. Place ...floating, you do not need to necessarily maneuver the bottles or tubes to identify them. Once the water bath ...temperature, remove the lid and place your tubes or bottles into the water bath. Many items may float in the...