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We narrowed to 663 results for: PPL;

Showing: 241 - 260 of 663 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this... this blog we will focus on those which can be applied in cell culture and animal models primarily. Fun...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...

  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...Fishell   Optogenetics Optogenetics continues to supply neuroscience with ever more efficient tools for...

  3. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...with the same genetic background and do an apples-to-apples comparison of all those mutations. If you ...different genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare...

  4. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...research questions. CombiGEM has been successfully applied to functionally characterize combinatorial gene...realization of a variety of perturbations and applications in functional genomics, cell reprogramming, ...He is particularly interested in developing and applying new technologies to interrogate and understand...

  5. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ... Tang and Cepko were eager to see if they could apply GFP regulation to other types of proteins. They ...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...to increased intracellular calcium. It has been applied to mark active populations of neurons in vivo to...Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction to AAV ...

  7. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ... reduced off-target editing frequencies. For applications that require high DNA editing specificity, for...deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved base editing...editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038...

  8. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... experimental, biotechnological, and medical applications. Researches can visualize proteins in vivo by...protein you need   Your plans for downstream applications In this blog post we will summarize some of...acids that are useful in different downstream applications. Cell-free expression systems however, are expensive...

  9. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate ... of chromatin interaction networks  A second application for CAPTURE 2.0 is the construction of spatial...temporal changes in chromatin interactions A third application for CAPTURE 2.0 is detection of temporal changes...

  10. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...accuracy of assembly. The fidelity data can be applied to derive similar high-fidelity overhangs for any...Kucera is a Principal Development Scientist of Applications & Product Development focusing on DNA cloning...Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias...

  11. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...not-so-technical skills you’ve built over time are only applicable to those positions you have already held. Imagine...have the desired degree, you have mastered the applications they use, you’ve gained experience working with...identified by another person. Similar reasoning may also apply to other acquired skills.” It is nice when other...

  12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... use ddPCR for AAV titration, there are many applications for the technology.  Droplet digital PCR is ...being used in the detection of microbes. Some applications include measuring viable probiotics and detecting...detecting circulating pathogens.  With its many applications and ease of use, droplet digital PCR is becoming...

  14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...the CNS (figure 5 in Chan et al) and the PNS (supplemental figure 8 of Chan et al). These new AAVs can ...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments... transgenic mouse required (see figure 5 and supplemental figure 8 in Chan et al).  References 1. Chan...

  15. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...acetylation) - tri_methylation In the next section we’ll apply the above formula to a real-world experiment. We...therapeutics. We’ll have more to say about clinical applications of CRISPR in the next section. In order to denote...is the foundation of precision medicine. We can apply this knowledge to create targeted disease therapies...

  16. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...it down until you have something tangible and applicable to your situation.   Thinking Outside the Box... currently plaguing the scientific community is supply chain shortages. When flasks, conical tubes or ...creative skills you have developed in the lab can be applied anywhere! References and Resources References ...

  18. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Protein A and Protein G are not suitable for all applications, such as protein isolation from serum. In this...capture antibody interaction and/or the downstream application. Typically, if the target protein will be detected...and an isotype control antibody. Alternative applications Oftentimes, an IP-appropriate antibody simply...

  19. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...multiple harvest strategy is that each day you are supplying your producer cells with fresh medium. Daily medium... freshly collected lentivirus for downstream applications. While this ensures that the highest titer virus...establishes the best conditions for specific applications, stick with them! Changes in such variables ...

  20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...property across various software providers used to supplement their paper notebooks. Instead, all of the important...might be available as well. In most cases this applies to universities. How are you currently exchanging... Besides this being of crucial importance when applying for a patent for example, it also comes in handy...
Showing: 241 - 260 of 663 results