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We narrowed to 601 results for: Ret;

Showing: 241 - 260 of 601 results

  1. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...than pre-designed sets, so identical searches can return multiple answers. You might want to take advantage...fragments, or defined split regions. SplitSet will then return the highest-fidelity overhang set it can find for...

  3. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ..., and Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human T lymphocytes for...

  4. CRISPR Guide

    Type
    Collection
    ...coding sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to...AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells that are poorly...Cas9 Fusion Tools dCas9 cannot cleave DNA, but it retains the ability to find specific DNA targets through...specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities but are small enough to...23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...: 32572269 Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A., Lamribet, K., Dardillac, E., Boix...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...depending on the cell line so old cells should be retired! Conversely, cells younger than the 3rd – 4th passage...

  6. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...scientists have taken advantage of the natural secretion of biochemicals and proteins through plant roots... Tagging recombinant proteins with a naturally secreted plant peptide allows for easier access and purification...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...fluorescence falls within the linear window for the theoretical timer shown in Figure 1B, you can use the equation...differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature communications 7 (2016...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...activators or repressors. These fusion proteins retain the ability to recognize and bind to DNA; when ...occasions truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, ...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...control of biological systems (Wheeler et al., 2016; Duret et al., 2019). But the field has sometimes seen ...plasmid here!   References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas...

  10. Of Myc and Men

    Type
    Blog Post
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...standard curve, and most results can be confidently interpreted as integers. Since each sample is partitioned...method you choose, resist the temptation to over interpret your results: Sybr cannot be used to determine...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

  15. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...within the target window, possibly due to longer stretches of duplexed RNA. Using an ADAR2DD catalytic mutant...perform C-to-U edits, the authors note that it retains its adenine deaminase ability. The authors then...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...appear reduced in ExM, which could impact how you interpret a target’s structure. For example, cytoskeletal..., Chen F, Piatkevich KD, et al (2016) Protein-retention expansion microscopy of cells and tissues labeled...

  18. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... great idea to most people in 2005, but XKCD is pretty successful!  What resources do you have?  No matter...for children” or may need text for audience interpretation. Infographics Low Combines both words and...

  19. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... mechanism to culture only bacteria which have retained a functional copy of your plasmid of interest,... plates due to the fact that beta-lactamase is excreted. Satellite colonies are small colonies of bacteria...

  20. Tips for arabidopsis transformation

    Type
    Blog Post
    ... (Kyndt et al., 2015)! It sounds simple, and it pretty much is, but there are some tips and tricks you...having an analyzable transgenic in hand, so it is pretty important to get this process right the first time...
Showing: 241 - 260 of 601 results