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Showing: 241 - 260 of 583 results
  1. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...plot. In this post, I’ll walk you through how to interpret the antibody readout on a flow plot.  Note that...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...
  2. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application...attenuated, nef-deleted HIV-1 strains in vivo. Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-...
  3. How Addgene Creates Content

    Type
    Blog Post
    ...quickly." Final design (right). Immediate and immense regret of the graphic designer not shown.   However... and knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid...
  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...website resources that best match a user’s query, and return the matching resources in an order that makes sense...expose as filters and designed the search engine to return only the filters that are relevant to a specific...
  5. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Editors carrying the delta-984-1090 ADAR2 truncation retain RNA editing capabilities but are small enough to...
  6. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...plan for a career outside academia (that would be pretty much all scientists), a LinkedIn profile is absolutely...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus...
  7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such as human growth...microenvironments! Take myoepithelial cells for instance. They retard tumor formation by inhibiting cell growth, invasion...
  8. RNA Extraction Without A Kit

    Type
    Blog Post
    ... and RNA is retained in the top, aqueous layer, while DNA and other proteins are retained in the interphase...
  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...fiction story that doesn’t require any detailed retention of information or formulation of new knowledge...mental bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise...
  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA...
  11. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...few commonly available different fragments that retain antigen binding (Figure 2):  F(ab')2 - These fragments...fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within the hinge ...
  12. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have...to permanently induce expression? Check out our Retro expression vector and Lenti expression vector. ...
  13. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...gender analysis and 2) provides case studies with concrete examples of how gender analysis leads to discovery...design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks to...
  14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...
Showing: 241 - 260 of 583 results