We narrowed to 302 results for: SPI

1. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

   ...physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene products were...

2. Filming Science Videos in the Age of Social Distancing

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   Blog Post

   ...during the middle of lockdown, and some that will spin up real soon. Video production, just like science...

3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   ...wheat, mushroom, and rice (Khatodia et al., 2016). Despite the almost universal functionality of the CRISPR...

4. Using Phosphoserine to Study Protein Phosphorylation

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   Blog Post

   ...organisms lacked a classic cysteinyl tRNA synthetase, despite abundant incorporation of cysteine in their proteomes...

5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   ...strategy may be a better option than direct cleavage. Despite the potential difficulties in translating these...

6. Treating Muscular Dystrophy with CRISPR Gene Editing

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   Blog Post

   ...wasting leading to death at around 30 years of age. Despite the amount of research conducted on DMD, there ...

7. Thoughts on the Future of Research 2015 Boston Symposium

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   Blog Post

   ...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

8. Is this the right place for me? 8 tactics for choosing a lab

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   Blog Post

   ...Will you learn how to run a lab if that is your aspiration? Will you help or at least observe grant writing...

9. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves...

10. Plasmids 101: Repressible Promoters

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    Blog Post

    ...to the promoter and turning transcription OFF. Despite their bacterial origins, Tet systems function well...

11. Hot Plasmids: Spring 2025

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    Blog Post

    ...expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked...

12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

    ...through our in-stock viral prep service.  AAV2 Despite being isolated second, AAV2 is the best studied...

13. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post

    ...described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register parts and designs, we ...

14. Important Considerations When Using AAVs

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    Blog Post

    ...to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are not pathogenic...

15. Quick Guide to All Things Lentivirus

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    Blog Post

    ...order to aid viral binding. This method, called spinoculation, has been found very effective for T cells transduction...

16. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...Boyden et al., 2005; Deisseroth et al., 2006). Despite its relatively recent development, the history ...

17. 9 tips for a successful postdoctoral experience

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    Blog Post

    ...new pieces of data, your talk will probably not inspire you or the audience. Ask yourself: What is the...

18. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ...

19. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ...these containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers...

20. Antibodies 101: Validation

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    Blog Post

    ...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...