We narrowed to 729 results for: STO;

...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

...sequence of a mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent...success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG.”  ...acceptor and donor sites, and introducing premature stop codons.    New from the viral service By Jason...

...sequences from a shared database of custom common features. Second, customers have asked for a way to annotate... involves iterative refinements in response to customer feedback. An example of this approach is SnapGene...

...of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine, this post describes..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs. Note: Casilio ...

...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...displayed at the individual read level, and because custom primers or "primer-walking" reactions are completely...problems. He received his PhD in Bioinformatics from Boston University, and did postdoctoral work at Harvard...

...to argue otherwise). To be sure, throughout the history of medical research, there have always been people...CRISPR/Cas9 research” but whether or not we will stop shuffling our feet on necessary actions to review...the future of genome editing technology. We must stop punting decisions on scientific, ethical, regulatory...

...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...recombinant E. coli on paramecia. European Journal of Protistology 48, 290–296 (2012). PubMed PMID: 22356923. 3... Polka, J. K. & Silver, P. A. A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo...

...them.   When you think about it, virtually every milestone in a scientist's career is mediated by some kind...flagship workshop.  Our 2018 workshop took place in Boston - you can revisit the activities of our attendees...careers in science communication The strength of story telling Science communication: 9 strategies to get...

...RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock ...from this paper are available at Addgene. Yan, Winston X., et al. “Cas13d Is a Compact RNA-Targeting Type...Plasmids Check out our CRISPR Guide Read our CRISPR History  ...

...assembled a cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist...particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...of our information in order to more granularly customize the search engine, and a scoping mechanism that...

...Addgene has developed in terms of the collection, storage, and distribution of the plasmids and their ability... Michigan, Ann Arbor and obtained her J.D. from Boston University School of Law, where she was on law ... of Advisors and Audit Committee at the Greater Boston Food Bank and participates locally in Read To A...

...design a custom primer. Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...

...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989.... While the codon used is often one of the three stop codons ­– most commonly the UAG codon – in some ... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

... addition, there was an entire story-telling session organized by Story Collider and which featured a ...the different parts of the brain stimulated by stories. In retrospect, I should have expected this given...

...in C++ and Java Command line history: Tracking command line history by recording all commands entered...addition to output files, this is also where you can store any power point, excel, or other files relevant ...

...project, I read the book “Every Other Thursday: Stories and Strategies from Successful Women Scientists... group to be intrinsic to their success. Their stories made it clear to me that, executed correctly, the...renowned Healthcare Businesswomen’s Association (HBA) Boston Chapter and the Massachusetts Association for Women...

...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August... studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated controls....researchers were able to examine each individual blastomere to determine its genotype. However, this detailed...

... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ...our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...

...faculty (at time of writing, Brigham And Women's in Boston also just announced that they were reversing their...does not appear to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and ...that are not raising salaries when all others in Boston (some of them literally across the street from ...

...Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters enable constant...2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage...Synthetic Biology: A Conversation with MIT’s Christopher Voigt Resources on Addgene.org See the synthetic...