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Showing: 241 - 260 of 734 results
  1. Future of Research Conference - Remarkable Opening Session

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    Blog Post
    ...postdoc-driven Future of Research Conference at Boston University last week.  This fantastic city-wide... supporters from all of the research centers in Boston.  I was relieved because it finally feels to me...program.  His strongest comments were about the astounding lack of reproducibility being recognized in published...
  2. Strengthening Foundations: Reflections on 2025 and What's Ahead for 2026

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    Blog Post
    ...making Laos the 112th country that we support. This story is a vivid example of how the global scientific ...advanced. Behind every publication is another great story, like researchers in India using Addgene-shared ...research. Thank you for letting us be part of your story, and for being such an important part of ours. We...
  3. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...should be able to tell your story for you and they should also be easily understood and consistent in design...Writing and Publishing Institute. Writing is the cornerstone of any scientist’s career. We use writing to ...
  4. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    Blog Post
    ...says. Percent sequence identity was a different story - the team found that the percent sequence identity...Addgene as a large data set. Alec A. K. Nielsen & Christopher A. Voigt also recently published a tool (trained...PMCID: PMC6200774.   2. Nielsen, Alec AK, and Christopher A. Voigt. "Deep learning to predict the lab-of-origin...
  5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post
    ...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...
  6. Hot Plasmids and Viral Preps - September 2021

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    Blog Post
    ...sequence of a mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent...success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG.”  ...acceptor and donor sites, and introducing premature stop codons.    New from the viral service By Jason...
  7. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine, this post describes..., and a modified gRNA to direct a variety of customizable functions to your DNA sequence of interest. ... how the Casilio system works and how you can customize it for your experimental needs. Note: Casilio ...
  8. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post
    ...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...displayed at the individual read level, and because custom primers or "primer-walking" reactions are completely...problems. He received his PhD in Bioinformatics from Boston University, and did postdoctoral work at Harvard...
  9. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    Blog Post
    ...to argue otherwise). To be sure, throughout the history of medical research, there have always been people...CRISPR/Cas9 research” but whether or not we will stop shuffling our feet on necessary actions to review...the future of genome editing technology. We must stop punting decisions on scientific, ethical, regulatory...
  10. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post
    ...enormous lattice of identical subunits. A brief history of R bodies R bodies have been studied for many...recombinant E. coli on paramecia. European Journal of Protistology 48, 290–296 (2012). PubMed PMID: 22356923. 3... Polka, J. K. & Silver, P. A. A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo...
  11. Communicating Your Science With Help From ComSciCon

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    Blog Post
    ...them.   When you think about it, virtually every milestone in a scientist's career is mediated by some kind...flagship workshop.  Our 2018 workshop took place in Boston - you can revisit the activities of our attendees...careers in science communication The strength of story telling Science communication: 9 strategies to get...
  12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post
    ...RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock ...from this paper are available at Addgene. Yan, Winston X., et al. “Cas13d Is a Compact RNA-Targeting Type...Plasmids Check out our CRISPR Guide Read our CRISPR History  ...
  13. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ...assembled a cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist...particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...of our information in order to more granularly customize the search engine, and a scoping mechanism that...
  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ...design a custom primer. Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...
  15. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post
    ...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989.... While the codon used is often one of the three stop codons ­– most commonly the UAG codon – in some ... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...
  16. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ... addition, there was an entire story-telling session organized by Story Collider and which featured a ...the different parts of the brain stimulated by stories. In retrospect, I should have expected this given...
  17. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post
    ...in C++ and Java Command line history: Tracking command line history by recording all commands entered...addition to output files, this is also where you can store any power point, excel, or other files relevant ...
  18. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post
    ...project, I read the book “Every Other Thursday: Stories and Strategies from Successful Women Scientists... group to be intrinsic to their success. Their stories made it clear to me that, executed correctly, the...renowned Healthcare Businesswomen’s Association (HBA) Boston Chapter and the Massachusetts Association for Women...
Showing: 241 - 260 of 734 results