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  4. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...dopaminergic neurons in a brain region called the substantia nigra, which is the main source of the body’s...sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar) DA affinity and high-magnitude responses (increases...

  5. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...

  8. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...reasons to feel obligated too, as experts on the subject brought out in a Kavli Foundation Round Table. ... be a good way to follow news about particular subjects or find out what others are reading. Check out...

  12. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...to what the research had to say. Moreover, this subject allowed me to engage in findings relevant to my...areas in which I knew very little. Diving into subjects like risk perception, scientific uncertainty and...

  14. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... be helping the untagged protein enter cells. Subsequent work found that both NaCl and non-detergent sulfobetaine... be used to transduce Cas9 and a gRNA, which subsequently enter the nucleus and cleave the specified target...

  15. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...hairpins into the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize... used to visualize relative locations of the subtelomeric region of the pericentromeric region (PR1), ...

  16. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can... lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating these ...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository... here!   Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

  19. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...conditions will derail a research program (think a subtle difference in mouse chow, a different water filtration...did. Admittedly, the differences are often very subtle, and it can take a lot of detective work to figure...

  20. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...bodies are a polymer of many copies of monomeric subunits. Unlike cytoskeletal polymers, however, R bodies...propagating through an enormous lattice of identical subunits. A brief history of R bodies R bodies have been...
Showing: 241 - 260 of 576 results