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We narrowed to 573 results for: Vit

Showing: 241 - 260 of 573 results

  1. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...considerable A → G editing activity at most sites, in addition to A → C and A → T activity. To drive selective...introduce T → C and T → G edits, though with limited selectivity between those outcomes, with an average efficiency...

  2. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...editing has quickly become a popular system for in vitro and germline genome editing, but in vivo gene editing... (SaCas9, 1053 amino acids) displayed cleavage activity in mammalian cells. SaCas9 produced indels at ...SaCas9 did not display higher levels of off-target activity than SpCas9, confirming its suitability for in...

  3. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... et al. "Near-perfect infectivity of wild-type AAV as benchmark for infectivity of recombinant AAV vectors...of 50:1 (Zeltner et al., 2010). The specific infectivity of viral preparations is defined by the ratio...

  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... the virus and is less affected.  Altering sensitivity to serum. Different pseudotyped viruses are differentially...studies since there’s the potential for off-target infectivity as it can bind and transduce many cell types ...body, making it a great tool to study neuronal connectivity. However, because of rabies virus’s toxicity...

  5. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis (those... can be to get a “bad batch”); Customizable sensitivity and detection - since they frequently undergo...Experiments Learn more about GFP Controlling Protein Activity with GFP Resources at Addgene.org Find the Spinach...

  6. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...consulting firms can be involved in many similar activities depending on the expertise of the senior leadership...of schedules can take on employee morale and productivity, and many have mechanisms in place to prevent...those who understand that these challenges are an inevitable part of the profession, and that they can be ...

  7. The Challenges of Cell Culture

    Type
    Blog Post
    ...most as cancer cells are amenable to culture in vitro. Virologists too have benefited from the ability...have plenty of maintained freezer stocks. It is vital that the Master and Working stocks of cell lines...Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture Basics Guide Abcam Cell Culture ...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...fluorescent proteins can be accurately determined in vitro by measuring the fluorescence intensity of purified...substantial size of GFP may interfere with the activity or the localization of the fusion protein. Also...Peptide-tags for site-specific protein labelling in vitro and in vivo." Molecular BioSystems 12.6 (2016): ...

  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... quantify the effect of mTOR inhibition on the activity of RNA Polymerase III. Red and orange fluorescent...then identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity...Katherine Deigan, et al. "Structural basis for activity of highly efficient RNA mimics of green fluorescent...

  10. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...designed solely for restriction enzyme sensitivity or resistivity, and thus be a marker for edited cells... Fig. 3: Restriction enzyme screening for resistivity to digestion, indicating targeted edits. ...

  11. March for Science

    Type
    Blog Post
    ... by reality – a government cannot declare that gravity change direction and make things fly up when we... believe climate change is mostly due to human activity while much less of the general public agrees (...Democratic values and their role in maximizing the objectivity of science.  Scientific American: Scientists ...

  12. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...with 5’->3’ polymerase activity (for amplification), 3’->5’ exonuclease activity (increases amplification...amplification fidelity), and no 5’->3’ exonuclease activity (which could potentially truncate the template). Importantly...

  13. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...well in a rat or non-human primate. Likewise, in vitro efficacy doesn’t always translate to in vivo efficacy...chemogenetic tools, where modification of cellular activity outside of your experiment site or circuit can...measuring expression over a period of weeks (in vitro) or months (in vivo).  Selective promoters Selective...

  14. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ... So never mind how much better your work and productivity will be if you’re part of a team - it will just...That’s a subtle but important distinction. If I invite someone to be on a project team, I try to make ...next team works better. In fact, don’t wait to be invited onto a team. Make one yourself. Instead of thinking...

  15. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... influence receptor conformation and signaling activity​​ (Sridharan et al., 2014). It's also crucial ...addition of fluorophores affects the functional activity of the ligands. For instance, studies have shown...(< 10 nm) and overlap of excitation profiles is vital for the accurate interpretation of FRET-based experiments...

  16. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... the known pathway that were essential for the activity of different ligands. Such knowledge could be ...effects. Typically the phenotype would be studied in vitro, but there is enormous potential in performing in...G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  17. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...exposed ssDNA bases. Thus, base editors have an activity window restricted to the stretch of ssDNA created... edits at certain positions within the editing activity window (Kurt et al., 2021). They hypothesized ...suggest that DSBs are a common intermediate of CGBE activity, and they are sometimes processed by error-prone...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    ... a weak signal. However, they have high cross-reactivity, vary significantly from lot-to-lot, and have...but they have higher specificity, lower cross-reactivity, and are more consistent lot-to-lot. The highest...usually preferred because of their higher specific activity, slightly lower cost, and smaller size. However...
Showing: 241 - 260 of 573 results