We narrowed to 407 results for: abo.4

...Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose ...pombe have dominated the research scene. But what about the other yeasts – in the biomedical world, are ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...

...mediated DNA damage is a DSB within the target DNA ~3-4 nucleotides upstream of the PAM sequence. Cas9 Species...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...

...transcriptional activation screening. Nat Protoc. 2017 (4):828-863. doi: 10.1038/nprot.2017.016. Additional...changes. Using this road map, and what was known about previously characterized TFs, the Zhang group were...Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your...

... 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental Microbiology... with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...

...experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and fluorescence in high molarity... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

...https://doi.org/10.2174/1389201021666200621161610 4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...

...points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond to customer help tickets. Catch up ...more about their needs and any problems that they are trying to solve. After learning more about the stakeholders...the company and then go to grad school. However, about a year and a half into my role, I realized that ... my product management (PM) career. Had I known about product management earlier on in my career, I’d ...interests. As such, I want to be able to share more about this nontraditional career path for others who love...management.  A day in the life  Now that you know a bit about what a software product manager does, you might ...

...30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn more about research...species (read Arbor Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx...Additional Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using...using Cas9 Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org...

...Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

...that can help you craft a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

... phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, R6K...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

...methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "Direct visualization of newly... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

...level of antibodies is reached - typically within 2-4 months. After the animal has the desired level of ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

...Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL ...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch...

...Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-66. PMID: 17888184; PMCID: PMC2075523. Bulcha, J.T...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

...PubMed PMID: 1495975. PubMed Central PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

...Improved signal-to-noise ratio (SNR). Figure 4: Calcium titration curves for OCaMP and various red... view of traces corresponding to the dashed box above. The number of action potential (AP) spiking events...

...activation and base editing applications.  Figure 4: Overview of CasMINI for genome engineering applications... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...

...in short term storage (think days to weeks) in the 4°C. If you’ll only be using it every few month or if...fluorophore for an experiment is easy, but what about those times you need more than one?   Multiplex ...