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We narrowed to 397 results for: abo.4

Showing: 241 - 260 of 397 results

  1. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ..., vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp. 99–103. Crossref, https://doi.org/10.1021... Multipart Assembly.” ACS Synthetic Biology, vol. 4, no. 9, American Chemical Society (ACS), 1 May 2015... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila. ... Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024. Crossref, https://doi.org/10.1101...Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016...

  2. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...chromophore, resulting in emission of red fluorescence (4). The dimeric or tandem dimer versions of EosFP are...biology 12.3 (2005): 279-285. PubMed PMID:15797211. 4.Wiedenmann, Jörg, et al. "EosFP, a fluorescent marker...emitted, making it easier to capture a clear image above background. The majority of PA-FPs come in 2 flavours...Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent...

  3. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Something in their world should be fast and affordable. 4. Stem cell scientists are generous Besides some of...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent role in the ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they...

  4. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...be a major decision. A science trainee will spend 4-7 years working for one person and with a group who...during the day? We asked three lab heads how they go about selecting new hires. This is followed by some perhaps...visits the lab for a full day, gives a 1 hour talk about his or her interests, and spends one-on-one time...current members to share short-term goals and chat about awesome science. Important factors in the search..., the lab has a reputation for not entering collaborations. Alternatively, perhaps the lab publishes and...Medical School) for taking the time to speak with us about adding new scientists to their labs. Read the Other...

  5. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...restriction enzyme sequence resource is universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety...saved and cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook...enzyme, or name to discover all the information about that restriction enzyme or share it via email with... this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS... new researchers or students beginning to learn about biochemistry. The least this app will do is allow...

  6. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...recombination. In most recombineering strategies, only 3-4 phage proteins are needed and these can be provided...from previously discussed methods. As described above, you design two primers, each containing the desired...suited for insertions and deletions. The figure above shows guidelines for primer design; a desired insertion...mutation-containing colonies/total colonies) is about 84% for each of the three methods. However, REPLACR-mutagenesis... Additional Resources on the Addgene Blog Read about Other Plasmid Cloning Techniques like SLIC and Golden...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose ...pombe have dominated the research scene. But what about the other yeasts – in the biomedical world, are ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...

  8. Components of CRISPR/Cas9

    Type
    Blog Post
    ...mediated DNA damage is a DSB within the target DNA ~3-4 nucleotides upstream of the PAM sequence. Cas9 Species...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...

  9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...transcriptional activation screening. Nat Protoc. 2017 (4):828-863. doi: 10.1038/nprot.2017.016. Additional...changes. Using this road map, and what was known about previously characterized TFs, the Zhang group were...Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your...

  10. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ... 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid Serotype Name 114471 AAV2...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  11. Hot Plasmids - January 2023

    Type
    Blog Post
    ...experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains folding and fluorescence in high molarity... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

  12. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental Microbiology... with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...

  13. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...https://doi.org/10.2174/1389201021666200621161610 4. Uddin et al. 2020.  “CRISPR Gene Therapy: Applications...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...

  14. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...points we’ve identified. I can’t wait to send it out. 4:00 PM--Respond to customer help tickets. Catch up ...more about their needs and any problems that they are trying to solve. After learning more about the stakeholders...the company and then go to grad school. However, about a year and a half into my role, I realized that ... my product management (PM) career. Had I known about product management earlier on in my career, I’d ...interests. As such, I want to be able to share more about this nontraditional career path for others who love...management.  A day in the life  Now that you know a bit about what a software product manager does, you might ...

  15. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

  16. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn more about research...species (read Arbor Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx...Additional Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips for using...using Cas9 Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources on Addgene.org...

  17. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...that can help you craft a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

  18. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "Direct visualization of newly... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

  19. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...level of antibodies is reached - typically within 2-4 months. After the animal has the desired level of ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

  20. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ... phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, R6K...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...
Showing: 241 - 260 of 397 results