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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 315 results for: bon;

Showing: 241 - 260 of 315 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...pyruvate, Corning 10-014-CV (optional) 7.5% sodium bicarbonate, Corning 25-035-CI (optional) 1 M HEPES, HyClone... 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose...other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers...

  2. Biosensor AAV Preps

    Type
    Collection
    ...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51083 AAV-EF1a-DIO-GCaMP6f-...GSG-P2A-GCaMP6f-WPRE-pA CAG GCaMP6f mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51082 AAV-EF1a-DIO-GCaMP6s-...GSG-P2A-GCaMP6s-WPRE-pA CAG GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 68720 pAAV-hSyn1-Flex-mRuby2...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with the Voytas Golden...introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences...mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (...with the Voytas Lab Golden Gate TALEN kit. This backbone is suitable for the TALEN-based generation of ...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...to top Recombinase-Dependent Empty Backbones Find an empty backbone to regulate your gene of interest....Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific... other recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase...

  9. Typing CRISPR Systems

    Type
    Blog Post
    ...Laderman, E., Armbrust, M., Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...experiments.       References and resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence...

  15. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...desired guide RNA sequence by digestion of the backbone with BbsI followed by a quick ligation of annealed...and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene...

  17. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...primers. These primers typically anneal to the backbone and can help you verify the ends of the insert...primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for reference...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ...This includes plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, ...selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources...pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing...

  19. Ligation Independent Cloning

    Type
    Protocol
    ...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation...design for LIC is often as simple as using the backbone manufacturer's suggested leader sequence fused...
Showing: 241 - 260 of 315 results