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We narrowed to 335 results for: c rel

Showing: 241 - 260 of 335 results

  1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...Bauer DE, Kamran SC, Lessard S, Xu J, Fujiwara Y, Lin C, et al. An erythroid enhancer of BCL11A subject to...of the method. One observation was an inverse relationship between deletion size and frequency [3]. There... DNA function is typically inferred based on correlative biochemical characteristics and ectopic reporter...   Like the video? The Orkin and Bauer lab's related paper describes their methods for CRISPR design...

  2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...annurev-pharmtox-010814-124803 Vlasov, K., Van Dort, C. J., & Solt, K. (2018). Optogenetics and Chemogenetics...level of control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs...throughout the body. Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation... of being less invasive and more flexible as it relies on designer ligands that can be simply introduced...

  3. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...In mammalian cells such as stem cells, HDR is relatively rare and DSBs are predominantly repaired by NHEJ...either the PAM (changing one of the G’s to an A,T or C – but avoid NAG) or guide RNA target (“seed”) sequence... or guide RNA blocking mutations? This depends entirely on the locus you are editing, and if the blocking...experiments with blocking mutations, we still weren’t entirely happy with our editing outcomes and in many cases...mutation were on the same oligo, the intended mutation rarely got in. It seemed that the cell was only using ...characterize this phenomenon and found a general relationship between the distance of a mutation from the ...(and let them cut close).   Figure 2: Relationship between cut site to mutation distance and editing...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...population. Heat maps are often used to indicate the relative density of cells. You can see both of these features...Barlow-Anacker, A., Bochkov, Y., Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16...are used to show frequency of events, instead of relying on clustered dots. In a contour plot, each line...intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic...

  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...transfection. This supernatant can be stored at 4 °C or can be directly added to your target cells. However...and plasmids.  References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging...vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic liposome) or electrical...Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant and...viral capsid antigen p24, the FACS dosing method correlates viral titre to the number of cells transduced...

  7. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ... Kunjapur, Aditya M., Philipp Pfingstag, and Neil C. Thompson. "Gene synthesis allows biologists to source...slight or large variations depending on how closely related these species are. If a gene was moved exactly ...exactly as is from one organism to a distantly related organism, the gene may not express well because of these...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then...growing trend of sourcing genes from distantly related organisms. In 2006, less than 1% of the genes deposited...applications in monitoring the accidental or intentional release of engineered organisms. “Is [the organism] engineered...

  8. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...promoter substitution), and tagging at either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et ...) PLoS ONE 8(7): e67902. Many imaging studies rely on direct fusion of fluorescent proteins (FPs) to...

  9. mTOR Pathway

    Type
    Collection
    ...PKC eta PKC iota PKC theta PKC zeta Protein kinase C Protor Also known as PRR5; proline rich 5 PTEN Phosphatase...signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers. Cancer...Enhanced signaling through either mTORC1 or mTORC2 correlates with poor prognosis in many cancers, including...for clarity and does not indicate a specific relationship. The content for this page was generated with...tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known as RPTOR; Regulatory...

  10. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...under the United States Internal Revenue Code 501(c)(3). That means we were formed to benefit the public...confidentiality where it is appropriate. This grows our relationship with our community members. We also find that..., Susanna, Tenisha, Thomas, Tony, Tracy, Tyler, Tyrel, Wogenie, and Xuan Additional Resources on the Addgene...

  11. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...) antibody-dependent cellular cytotoxicity (ADCC) c) antibody-dependent cellular phagocytosis (ADCP). ...cells expressing Fcγ receptors binds to IgG and release cytotoxins that kill the target cell. If the pathogen...antibodies and eosinophils, where the eosinophils release cytotoxins via degranulation.  Antibody-dependent...

  12. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...field! References and Resources References Fellmann, C., et al., Cornerstones of CRISPR-Cas in drug development... or have a simple pathology. In these cases, preliminary identification of potential targets by CRISPR...researchers to select and generate physiologically relevant models. In complex diseases, it can aid in generation...

  13. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    .... DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying the known and unknown microbial...traps? Traps – gene, enhancer, and promoter – all rely on the random integration of a reporter construct... effective tools but neglect some biologically relevant situations. Tissue-specific genes – the genes...

  14. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...signaling dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation...the mitochondrial targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported...pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP...N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and plant resistance...compartment of action via sequestration. The strategy relies on several observations: Rapamycin is a membrane-permeable...physiological setting. Genetic techniques typically rely on overexpression or knock-down, where signaling...or small molecules and exceed a physiologically relevant exposure in intensity and/or duration. Both of...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver...GMP) FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-...Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science. 2010...Plasmids Joel Kralj Voltage Voltage sensors for use in C. elegans (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange...sensor (MARIO) A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...

  16. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...allow the digestion to take place for an hour at 37°C. This should be enough time to completely digest the...time requirements. FastCloning is a simpler yet reliable cloning technique that was developed by Li, et...

  17. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...of our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the...pDEST-HisMBP). After purification the protein can be released from the resin and used for various binding or...

  18. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...https://doi.org/10.1038/s41598-020-78827-9 Breen, C. J., Raverdeau, M., & Voorheis, H. P. (2016). Development...Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J....60 minutes of illumination, highlighting its reliability for long-term experiments (Salari et al., 2019...unconjugated dye molecules are critical for accurate and reliable imaging and assay results (Hayashi-Takanaka et...

  19. Evolution of Lab Techniques

    Type
    Blog Post
    ...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...1975): 503-517. PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test...next-generation sequencing techniques do not typically rely on Sanger sequencing but instead use methods like...

  20. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M, Merten O-W, Blouin V, François A, Redon...preparation approaches described previously require a preliminary double stranding step. The double stranding step...preparations and showed that VGS can quickly and reliably confirm the identity of AAV genomes and detect...the Addgene blog Read all of our viral vectors related blog posts Read our blog post on AAV titers Browse...
Showing: 241 - 260 of 335 results