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We narrowed to 1,045 results for: fer

Showing: 241 - 260 of 1045 results

  1. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...digestion be sure that the buffers and temperature are compatible between the different enzymes. Always check...ligation mix in different bacterial strains (stbl2, NEB stable, etc.), and/or use a different temperature ...want to assemble together in a combination of different ways according to your cloning strategy: Restriction...from other DNA fragments and you will remove the buffers used in the previous reactions. This will increase... There are multiple ways you can assemble the different parts of a plasmid based on the cloning strategy...attention in the areas of ligation between the different fragments If you don’t get any positive clone...might want to reconsider your approach, and try a different cloning strategy. Once you've identified potential...

  2. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...current job situation. What can a coach do that is different from an adviser, boss or mentor? First, professional... transition out of the academic sphere into a different meaningful career in science. Supervisors in the...speaking at research institutions and at scientific conferences, I see that few scientists are aware of the benefits...few hours of coaching time can make an enormous difference so don’t assume that this will cost a fortune...successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who have had a ...offices or professional organizations can provide referrals. There are bad coaches out there, so finding a...specializes in scientist coaching. Always ask for references and follow up with 3-4 to hear about those clients...

  3. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...containing structural genes and a transfer plasmid carrying the transfer gene. By dividing the viral elements...it is recommended that you try a few different ratios of transfer, envelope, and packaging plasmids in...associated with producing and using lentivirus and offer some tips and tricks for overcoming these hurdles...transactivator, Tat. For more information on the different generations of lentiviral packaging systems please...Guide. While the third generation packaging systems offer an extra level of safety, they also require a fourth...encoding the structural elements, envelope, and transfer gene are transfected into a packaging cell line...titer. Importantly, optimized conditions for one transfer plasmid are not always the best for others. For...

  4. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ... Evaluating Gene function by RNA interference (RNAi) RNA interference (RNAi) is a powerful tool for performing... These smaller dsRNA fragments are referred to as small interfering RNAs (siRNA) and they bind to a class...genes. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to knockdown expression of specific gene...morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids to investigate for ...WISH technique. The expression patterns of 11 different planarian genes (all of which are available through...one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia dorotocephalacan...and if you make new plasmids for investigating different planarian genes, we encourage you to deposit them...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...fear of referencing a wrong section. The antibody screening and development of a focused reference bring...School of Medicine at Mount Sinai.  References and resources References Baker M. Reproducibility crisis: ...), variability (separate batches can perform differently – happens when the antibody is produced from ...a new set of animals), and wrong application (different experiments/conditions can change binding ability...off-target molecules), reliable (wouldn’t perform differently over time), compatible with the desired applications...and accuracy while screening. We also compare different clones of the same target to see if the results...with general non-specific binding. If the sample differs from the negative control, such as showing a very...

  6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...for contributing their expertise! References and resources References Aschauer, D. F., Kreuz, S., & Rumpel...Stable Pancreatic Gene Transfer by Adeno-Associated Virus Vectors via Different Routes. Diabetes, 55(4...multiple coreceptors, which may be present on different cell types and sometimes vary between target species... numbers separated by a slash: the first number refers to the genome, and the second number to the capsid... al., 2021 In addition to tissue tropism, different AAV serotypes can be useful to evade neutralizing... (AAV) Serotype 9 Provides Global Cardiac Gene Transfer Superior to AAV1, AAV6, AAV7, and AAV8 in the ...., Yin, L., Merigan, W. H., Flannery, J. G., & Schaffer, D. V. (2013). In vivo-directed evolution of a...

  7. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For each of these...controlling properties like growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs...cells one at a time, you could identify those that confer resistance to drugs,” in a rapid, straightforward.... “You could use it to screen for pathways that confer any phenotype of interest, not just drug resistance...morphological features, migration, or survival in different contexts.” It’s also possible you could make use...plasmids in living animals, to explore pathways that confer metastatic potential or the ability to survive ... sometimes drug screens and sometimes totally different experiments we never thought of - and that’s thrilling...

  8. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...the non-profit sector are transferrable to industry such as technology transfer, communications, medical...skills overtime for success. Many people will have different roles, titles, and responsibilities during their...candidate is ultimately looking for a role in a different functional area, then the research role could ...higher degree. These numerically limited visas are referred to as “cap” subject H-1Bs. The H-1B allows U.S...and therefore the academic H-1B visa cannot be transferred to an industry position. If you cannot get an...a foreign national to have an employer or a job offer in order to obtain permanent residence. The recently...your field. No employer is necessary and no job offer is needed for this category. To qualify, you need...

  9. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...cellular proliferation. We have appreciated for some time the causes of this uncontrolled proliferation, in...normally encourage cell survival, growth and proliferation, while tumor suppressors perform the opposite...or, in the case of cancer, uncontrolled cell proliferation. Hence it is imperative to the health of an ...division. The combined effect is unregulated proliferation. Another safety net is our own immune system...cellular pathways involved in the development of different cancers and identifying the roles of key proteins...is the Ras pathway. The term “Ras” collectively refers to 3 proteins (H-Ras, K-Ras, and N-Ras) encoded...approaching the Ras problem by exploiting the different mutant versions of Ras that are most prevalent...

  10. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...presenting your research at conferences such as SACNAS and ABRCMS. These conferences have a lot of programming... recruiters will attend these conferences to answer questions and offer application fee waivers. One of...her on Twitter @EleanorWang.     References and resources References Canning EA, LaCosse J, Kroeper KM...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired by...proactive Rather than applying only to posted job offerings, the best research experiences typically come ... is a supportive environment—it makes all the difference in a lab experience. “Mentorship” was a bit of...interested in doing research and attending research conferences. The Barry Goldwater scholarship is also a national...

  11. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...primary antibodies raised in different species, or at least from different IgG subclasses, so that secondary...incubation of 1° and 2° Must use different species and/or different IgG subclasses of 1° for multi-color...limited cross-reactivity between different IgG subclasses and different species, nanobodies are useful ...Multiple polyclonal secondary IgGs can bind to different epitopes of a primary antibody. B) Each arm of...they don’t require using primary antibodies from different IgG subclasses. Instead, you can pre-incubate ...Once you know the answer to these two questions, refer to this table to find the right nanobody for your...come in 1x and/or a 3x cysteine varieties. This refers to the number of cysteine sites the nanobody has...

  12. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...capture the fact that different parts of the world encountered COVID-19 at different times. Most notably...plasmid requests Figure 5: Inferred effects. Values are the inferred percentage of normal activity ...scientific fields could be different.  Conclusion Figure 7: Mean inferred effects by region. In...also eases the interpretation of the parameters we infer, since scientific productivity is better interpreted...regions. Each of these regions was affected at a different time by COVID-19, so we defined the COVID-19 event... day) on dates impacted by each effect. The inferred parameters suggest that COVID-19 induced a 2x–...Europe most impacted and China least impacted. The inferred weekend effect is, in retrospect, generally more...

  13. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...serve similar purposes, both techniques offer researchers different advantages and limitations.  The four...factors in which chemogenetics and optogenetics differ are: Timing Targeted manipulation Controlling ...study. Figure 1: A schematic showing the differences in chemogenetics and optogenetics in a mouse ... light (optogenetics).   Timing Optogenetics offers a high degree of temporal resolution as receptors...neurons to repolarize faster.  Chemogenetics does not offer the same level of control over timing. Because chemogenetics...amounts of stimulation to receptors. Optogenetics offers better control over these factors as the light ...manipulated or turned off. In chemogenetics, while different concentrations of ligands can be injected, this...

  14. How to Write a Scientific Review Article

    Type
    Blog Post
    ...going to cite hundreds of references) and it’s better to keep your references organized from the beginning...sense of the similarities and differences among proteins in the class. Offer your perspective It doesn’t... tool kit. I wrote a review article on histone modifers in B and T cells with my adviser when I was a ...criteria to shape the document. Get and use a reference management program (e.g., EndNote, Papers, Mendeley...Mendeley, etc.) You’re going to be managing a lot of references. I cite as I write, meaning I use the software...in real time as I wrote, so each statement was referenced even in the roughest version of the draft and...that people can go back and read the original reference for the data. For example, we summarized how frequently...

  15. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...methods can interfere with binding, and some common antibody buffer components can interfere with conjugates...scientists have since adapted it for a host of different proteins and small molecules from a variety of...needs. Herein, we’ll give you a rundown of the different types of ELISA, advantages and disadvantages of... non-overlapping epitopes so that they do not interfere with each other. The detection antibody may either...this method, the target antigen competes with a reference antigen for binding to a primary antibody.  While...typically users first coat the multiwell plate with a reference antigen and then they preincubate the antigen-...allow the unbound primary antibody to bind to the reference antigen. The more antigen in the sample, the more...

  16. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...with that…and happy flowing!    References and resources  References Alyamani, A., Kalamegam, G., Ahmed...usually tell where this point is by looking at the different populations. In the case of Figure 2, one population...   Figure 3: It's often easier to identify different populations by imagining (or drawing) a quadrant...population and everything outside the gate is a different population.  Gating and plot orders  As the gates... right.  First, SSC and FSC are measured to differentiate cells from debris. Cells are gated, and in the...dendritic cells is further separated into three different populations using conjugated antibodies against...help me as a reader if I'm struggling to see the different populations on a plot.      Figure 5: ...

  17. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...be translated to other lines. Differences in the physiology of different cell types, however, can make...not only between serum from different manufacturers but also between different lots of serum from the same...low levels of contamination. For those labs that prefer a more economical mode of detection, Uphoff et....the second. When harvesting lentivirus, some labs prefer to collect and pool the harvests from multiple ...for a single harvest after transfecting with the transfer and packaging plasmids. A benefit of the multiple... as the transgene expressed, the viral storage buffers used, and other experimental parameters. For example...concentrated dilutions you will be able to overcome differences in receptor expression. It's all in the mix Even...

  18. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...converted to a different isotype, then two different secondary antibodies can be used with different fluorescent...have different binding properties (Morelock 1994). While in the majority of cases the differences are ...conversions and chimeric antibodies! References and resources References Liddell, E. (2013). Antibodies. In...changing an antibody’s natural isotype to that of a different group (Fig. 1). This process, called isotope conversion...antibody’s isotypes, however, you could then use 2 different secondaries for your antibody pair and visualize...constant regions or solely the Fc region of a different species and/or isotype (Liddell 2013). In most...

  19. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...dopamine sensors offer different levels of apparent dopamine affinity, with 113050 offering high apparent...these readily available to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey... various AAV serotypes can be used to achieve different patterns of expression, researchers often need...empirically for their own experiments. To this end, we offer specific control AAV in smaller aliquots of 20 ul.... The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes. See...sensitive response to action potentials, is now offered under control of the CAG promoter for ubiquitous...monitoring of neurotransmitters and we are excited to offer AAV for the newest GRAB sensors from the Li Lab....

  20. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...experiment uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers...analysis. Since different populations of immune cells express unique Clusters of Differentiation cell surface...flow cytometry experiments.       References and resources References: Bonner WA, Hulett HR, Sweet RG, ...  Cells are first resuspended in a pressurized buffer called sheath fluid and transported through tubes...each cell as it passes through and scatters in different directions. The degree of scatter is captured ...highly versatile and some can accommodate up to 30 different colors. This lends flow cytometry particularly...to complex experiments looking at a variety of different targets. If you are planning on using fluorescent...
Showing: 241 - 260 of 1045 results