We narrowed to 304 results for: human dat

...developing cell-free synthetic biology applications for human health and the environment.     Alexander A. Green...sequence information from public databases to the production of validated sensors in as little as one week...

...Protein Tags Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...of a protein and can inducibly facilitate the degradation of the tagged protein. Degrons are destabilizing...various enzymes and sent to the proteasome for degradation. The tags we will discuss here are all conditional...conditional, meaning that they only facilitate degradation of their fused protein under certain conditions...are reversible mechanisms of control. Protein degradation can be induced by the addition of a small molecule...to normal once the drug has been removed. The degradation and reversal are rapid compared to other systems... entire tagged protein to the proteasome for degradation. In addition to having the coolest name of all...

...classic technique to elucidate the mechanism by which SOX2 acts to reprogram human fibroblasts into induced...Mammalian Expression Construct SpCas9 Inhibition (Human Cells) acrIIA1 Listeria monocytogenes  pRAU168...Addgene blog to get all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying...subtilis libraries Transcription factor fusions elucidate iPSC reprogramming mechanism The Ptashne lab ... modular cloning steps. The Voytas lab plans to update the toolkit frequently and has developed a website...

... so that students see academics as well-rounded human beings. It’s also a great communication tool for...disease. They also devise biosensors designed to elucidate the molecular interactions that go on between ... What does it include? Patron: Sainsbury has a mandate to share and educate and enable plant science in...making all the tools for plant research here and validating them, it made the most sense to us to enable ... MoClo system. The paper also gives quantitative data for regulatory elements, which has not been available...

...adding a 9-residue peptide to the C-terminus of human codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis...containing a synthetic operon that enables the degradation of caffeine (1,3,7-trimethylxanthine) and its...

... Fig. 1: SpCas9 has sparse PAM coverage in the human genome. SpRY Cas9 has PAM sites covering the majority... off-target binding. The activity of SpRY was validated in double stranded cleavage and base editing applications...

...Check out some in-depth grant writing tips at the Human Frontier Science Program's website. One way to add..., or even the successful use of your preliminary data to secure further funding. Make it clear that you...

...Biology PhD Candidate at the University of Arizona. She studies the interaction between Human Papillomavirus... was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps you’re about...

...PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (...transcriptional and epigenetic regulation have been validated with these proteins. Furthermore, among those ...through the creation of innovative technologies. To date his group has develop several popular CRISPR-based...

...Greek to you, which brings me to my next analogy. Human languages evolve over time, for example from Ancient...up to date with their third party libraries and any security updates. Most of the time, updating these...need to update our code to be compatible with the updated library. A recent example is updating our software...would be staying on top of any third party library updates. Third party libraries are reusable software components...is no longer being maintained (i.e. hasn’t been updated in several years) or they make significant changes...this year, which means there will be no further updates, security or otherwise. This might sound like Greek...blog Learn about what went into our search engine update Find all Addgene news Resources on Addgene.org...

...to differentiate strains of Zika virus, genotype human DNA, identify tumor mutations within cell-free genomic...This post was updated on Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly...From this analysis, they identified 53 potential candidate genes that fell into 3 categories based on the...question: C2c1, C2c2 and C2c3 (short for Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1...Cary Valley and Mary Gearing contributed to the update of this post. References 1. Abudayyeh, Omar O.,... Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check Out Our CRISPR...

...fluorescent proteins and optogenetic tools exist, humans (like many organisms) are opaque, which makes most...website (and often posting updates on social media) and freely sharing code, raw data, and more. The team shares...mechanism, though they have collected a variety of data that might help formulate some hypotheses. They ...

...pipelines (e.g. using Tn5), and as knock-in tools for human therapeutics (e.g. using Sleeping Beauty). Despite...was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical...VchINT, at two different incubation temperatures. Data adapted from Vo et al., 2020. In E. coli, the...guest blogger Leo Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical...

...Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis. Journal...which HIV is a member — may seem like an unlikely candidate to use as a viral vector, but retroviruses have...lentiviral genome (right.)  HIV seems an unlikely candidate for use as a tool in the lab because it is, after...understand these seemingly odd choices and lay the foundation for producing and using retroviral vectors effectively...

...Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012. Additional... label your plates and/or flasks with your name, date, the cell line you’re using and its passage number...

... that there is an NGG PAM every 42 bases in the human genome. SpCas9 targeting should be easy, right? ...base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t know ...

...serotype 2-derived inverted terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: ... virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great...hours hands-on time and 2 hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by...

...not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi....resistance to both carb and amp, due to their common degradation mechanism. How to choose between the two? Carb...high pH environments and is less susceptible to degradation in agar plates. This prevents satellite colonies...

...building events like Escape the Room or Habitat for Humanity take you and your co-workers out of the office... up and running repository. As they built the foundation for what Addgene is today, there was a lot of...culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the question...

...Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. USA 112(10) (2015...protein. Such flexibility makes aptamers ideal candidates for biosensors. Aptamer biosensors are used in.... The nature of aptamers makes them excellent candidates for developing assays for otherwise difficult-to-study...