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We narrowed to 302 results for: human dat

Showing: 241 - 260 of 302 results

  2. Lab Automation at Addgene

    Type
    Blog Post
    ...is ideal because it eliminates the potential of human error or cross contamination between samples. With...Updated Apr 6, 2021. If you’re doing high throughput work, it’s likely that you come into the lab wishing...hand. We’ll be sure to tell you about more lab updates as we develop them. In the meantime, if you have...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...developing cell-free synthetic biology applications for human health and the environment.     Alexander A. Green...sequence information from public databases to the production of validated sensors in as little as one week...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Protein Tags Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...of a protein and can inducibly facilitate the degradation of the tagged protein. Degrons are destabilizing...various enzymes and sent to the proteasome for degradation. The tags we will discuss here are all conditional...conditional, meaning that they only facilitate degradation of their fused protein under certain conditions...are reversible mechanisms of control. Protein degradation can be induced by the addition of a small molecule...to normal once the drug has been removed. The degradation and reversal are rapid compared to other systems... entire tagged protein to the proteasome for degradation. In addition to having the coolest name of all...

  5. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...classic technique to elucidate the mechanism by which SOX2 acts to reprogram human fibroblasts into induced...Mammalian Expression Construct SpCas9 Inhibition (Human Cells) acrIIA1 Listeria monocytogenes  pRAU168...Addgene blog to get all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying...subtilis libraries Transcription factor fusions elucidate iPSC reprogramming mechanism The Ptashne lab ... modular cloning steps. The Voytas lab plans to update the toolkit frequently and has developed a website...

  6. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ... so that students see academics as well-rounded human beings. It’s also a great communication tool for...disease. They also devise biosensors designed to elucidate the molecular interactions that go on between ... What does it include? Patron: Sainsbury has a mandate to share and educate and enable plant science in...making all the tools for plant research here and validating them, it made the most sense to us to enable ... MoClo system. The paper also gives quantitative data for regulatory elements, which has not been available...

  7. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (...transcriptional and epigenetic regulation have been validated with these proteins. Furthermore, among those ...through the creation of innovative technologies. To date his group has develop several popular CRISPR-based...Addgene.org Check Out Our CRISPR Guide Pages Find Validated gRNAs for Your Experiments Get Ready-to-Use CRISPR...

  8. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...frequency to above 30% for a variety of targets in human cell lines, with an average indel frequency of only...catalyzes both cytosine and adenine base conversions in human cells. Nature Biotechnology, 38(7), 856–860. https...written by Mary Gearing in August 2016 and was updated by Jennifer Tsang in August 2020 and by Emily P... Browse CRISPR plasmids for base editing Find validated gRNAs ...

  10. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ... Fig. 1: SpCas9 has sparse PAM coverage in the human genome. SpRY Cas9 has PAM sites covering the majority... off-target binding. The activity of SpRY was validated in double stranded cleavage and base editing applications...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    ...Check out some in-depth grant writing tips at the Human Frontier Science Program's website. One way to add..., or even the successful use of your preliminary data to secure further funding. Make it clear that you...

  12. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...Biology PhD Candidate at the University of Arizona. She studies the interaction between Human Papillomavirus... was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps you’re about...

  13. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...Greek to you, which brings me to my next analogy. Human languages evolve over time, for example from Ancient...up to date with their third party libraries and any security updates. Most of the time, updating these...need to update our code to be compatible with the updated library. A recent example is updating our software...would be staying on top of any third party library updates. Third party libraries are reusable software components...is no longer being maintained (i.e. hasn’t been updated in several years) or they make significant changes...this year, which means there will be no further updates, security or otherwise. This might sound like Greek...blog Learn about what went into our search engine update Find all Addgene news Resources on Addgene.org...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...to differentiate strains of Zika virus, genotype human DNA, identify tumor mutations within cell-free genomic...This post was updated on Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly...From this analysis, they identified 53 potential candidate genes that fell into 3 categories based on the...question: C2c1, C2c2 and C2c3 (short for Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1...Cary Valley and Mary Gearing contributed to the update of this post. References 1. Abudayyeh, Omar O.,... Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check Out Our CRISPR...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ... CIneo-RL Renilla CMV Mammalian expression of humanized renilla luciferase Witold Filipowicz 100984 pGL4.18... animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...fluorescent proteins and optogenetic tools exist, humans (like many organisms) are opaque, which makes most...website (and often posting updates on social media) and freely sharing code, raw data, and more. The team shares...mechanism, though they have collected a variety of data that might help formulate some hypotheses. They ...

  17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...pipelines (e.g. using Tn5), and as knock-in tools for human therapeutics (e.g. using Sleeping Beauty). Despite...was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical...VchINT, at two different incubation temperatures. Data adapted from Vo et al., 2020. In E. coli, the...guest blogger Leo Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical...

  18. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis. Journal...which HIV is a member — may seem like an unlikely candidate to use as a viral vector, but retroviruses have...lentiviral genome (right.)  HIV seems an unlikely candidate for use as a tool in the lab because it is, after...understand these seemingly odd choices and lay the foundation for producing and using retroviral vectors effectively...

  19. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second  Edition), 2012. Additional... label your plates and/or flasks with your name, date, the cell line you’re using and its passage number...

  20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... that there is an NGG PAM every 42 bases in the human genome. SpCas9 targeting should be easy, right? ...base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t know ...
Showing: 241 - 260 of 302 results