We narrowed to 551 results for: nol
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TypeBlog Post...The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833...
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What the HEK?
TypeBlog Post...female. HEKs are generally cultured as an adherent monolayer, though derivatives have been adapted for growth...Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389/fbioe.2021.796991... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward the red...However, a challenge in optimizing base editing technologies across several cell types is the lack of a rapid... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...our network. We started with the idea of a biotechnology investment fund that would apply a percentage... biotechnology... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal... tagged proteins, a common problem of the AID technology (Yesbolatova et al., 2019). Find the mAID tagging... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...Ernesto Valdivia Silva, Universidad de Ingeniería y Tecnología (UTEC), Peru Julio Ernesto Valdivia Silva is ...synthetic biology resources and microfabrication technologies to generate a self renewable sensor, a bacterium... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post... NEB gave a brief presentation describing the technology. Then we split into pairs to actually try out...with a technician from Neon Therapeutics, a biotechnology company that uses neoantigen biology to treat... -
Cultivating Community Science at BosLab
TypeBlog Post...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...student at Boston University studying mammalian immunology, and in his free time, he is pursuing a plant... -
Science Career Options
TypeBlog Post...Writer Meteorologist Journal Editor Textile Technologist/Designer Fieldwork and traveling Many...desired, as roles are becoming more reliant on technology, and good computer skills will give you an advantage... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated... -
Hot Plasmids - January 2023
TypeBlog Post...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9...monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi:... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In this second...guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had nearly ... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...presented chronologically. With a resume however, most often people choose a reversed chronological style ... -
Hot Plasmids - May 2022
TypeBlog Post... for example). Given that the liver is an immunologically active organ, this advance is likely to afford...Kaufman, et al, 2022. This new barcoding technology would allow researchers to avoid the off-target... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...multiprotein complexes. Fig. 2. Multiplex immunolabeling with subclass-switched recombinant antibodies...monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:e43322. doi:... -
Hot Plasmids - February 2022
TypeBlog Post...live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting, and more....future genetic screens. DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items. The technology behind Addgene’s new search Like millions of... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post... is one of the latest developments in CRISPR technologies. It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...