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We narrowed to 522 results for: pes;

Showing: 241 - 260 of 522 results

  1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...drum. They have a wide variety of possible genome types that can be either double stranded (ds) or single...them far safer to work with than virions. The four types of viral vectors (which many researchers will informally...

  2. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...antibodies within certain classification groups called isotypes.  Why does isotype matter? Isotype matters when...secondary. If you were to change one of the antibody’s isotypes, however, you could then use 2 different secondaries...

  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...deliver a genes of interest to a variety of cell types in-vivo. These are widely used by the optogenetics...revolutionized how researchers make knockouts and other types of genome edits, but, even with CRISPR, it isn’t...

  4. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...re also distributing AAV preps with particular serotypes that can be used to deliver fluorescent proteins...future users. We’re excited to be distributing new types of materials. At the time of publication we’ve already...

  6. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...expression in target cells. While the various AAV serotypes can be used to achieve different patterns of expression... to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV inventory for the...

  7. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... et al. tested iTOP in a series of primary cell types using Cre and various loxP reporters. In both mouse...primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal and gut...

  8. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...comparing the transduction patterns of different AAV serotypes. To help make this easier for scientists, we now...testing. These samples are available in various serotypes of two popular control AAV encoding GFP. Check...

  9. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...improved transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the...blood-brain-barrier. In summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced...

  10. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether endocytosis, vesicle...explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis, vesicle...

  11. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so off-target...systems ensures that they can be used with many types of proteins, including Cas9 and dCas9-transcription...

  12. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...Connie Cepko to learn about her management style. Recipes for delegation success Remember that there are ...authority, responsibility and accountability? What types of issues do you want to know about right away? ...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...expressing plasmid. Envelope proteins determine which types of cells a virus can infect. The VSV-G envelope ...enables this virus to infect many different cell types so it is useful in a wide range of applications....

  15. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... with all the new biotechnology.” These are the types of inquiries that routinely hit the inbox at the...ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

  16. How Addgene Creates Content

    Type
    Blog Post
    ... creating and sharing knowledge, related to the types of materials we help distribute and the research...Then we format, do a final review to catch those pesky typos, and hit publish. Making content is an iterative...

  17. Antibodies 101: ChIP

    Type
    Blog Post
    ...experiences, but the increased binding can mask the epitopes antibodies recognize. It is therefore best to ...for one that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies...

  18. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... packaging, and transfer) are supplied by three types of plasmids, which are cotransfected into the 293T...flexibility to pseudotype γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope ...

  19. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...relatively low retrograde transport efficiency in most serotypes. Some viruses (e.g., rabies) naturally exhibit... be transduced equally well by AAV-retrograde serotypes (Skorput, 2022, Albaugh, 2020, Tervo, 2016).  ...

  20. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Tools blog can help you learn about the different types of viruses and pick one for your experiment. Research... of transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral...
Showing: 241 - 260 of 522 results