...cerevisiae to human: The important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed...nervous system. The fly has also emerged as an important human disease model with potential for therapeutic... scientists to easily control the level and spatiotemporal expression of a gene of interest. This type...due to their simplicity, yeast have become an important experimental model in the study of neurological... 1960s, the zebrafish has gradually become an important model organism. They share about 70% of their ...method that uses heat induction to control the temporal expression of different transgenes, the Stainer...

...of Brainbow, Brainbow 3.0 (Cai et al., 2013), incorporates the highly photostable fluorophores mOrange2...default, they are not fluorescent. Instead, they incorporate a truncated, non-fluorescent form of YFP as the... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was...overcame the low expression of XFP of Brainbow by incorporating a Tet-Off system to boost XFP expression. The...Tetbow can be introduced in vivo either by electroporation of plasmids, or using AAV vectors. The Imai... lab also developed a version of Tetbow that incorporates chemical tags instead of XFP to optimize it ...

...structure, separate chromosomes during mitosis, support transport of organelles and vesicles, and more. Although...microtubule-cutting sword Microtubules are one of the most important components of the cytoskeleton. They provide cells...demonstrated its use to probe cell mechanics, vesicle transport, and cell division, but given the long list of... what if you’re interested in that other most-important component of the cytoskeleton – actin? Actin dynamics...is not feasible or when the protein of interest supports a variety of processes in different cell types... (Aljiboury et al., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but...

...the start of every new year, it is a wonderful opportunity to once again reflect on our achievements in ... India, and Brazil. Through an expansion of our export license application service, we’re pleased to announce...by our depositors, used by our requestors, and supported by our repository. You can enjoy these stories...research materials and information. We will pursue opportunities to build on our strengths that have successfully...for the past twenty years while seizing new opportunities to create even greater value for the scientific.... In 2024, we will be actively seeking ways to support a global community where every researcher feels...

...scientists. Many members of the scientific community support open sharing. But, one critical part was missing...universities are showing an unprecedented amount of support about allowing use of plasmids for research by ... Technology Transfer Offices were, at least, temporarily closing, these groups were willing and able to...weeks. As of June 2020, we are happy to have the support of the following organizations to share materials... synthesized approximately 3,000 constructs to support SARS-CoV-2 research.  Browse the entire Ginkgo ...and funding initiatives such as Fast Grants that support us in our mission.  While rapidly expanding our...

...capstone project report and presentation, during the final week at Addgene. This report shared my work ...Coming from an environment with limited opportunities and support, puts into perspective just how much one... hearing her talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene...to differentiate which clone had successfully incorporated Syntaxin3 into its backbone. Once we had gotten...

...tasked with was creating and sustaining a biweekly report of our social media analytics, something that had... meant I was responsible for establishing a new report and routine across the marketing and content team...a fantastic spreadsheet template to build out a report on. I was able to understand what metrics would...this photo!  Here, I was also given a mixture of support and independence that allowed me to succeed. As...achieved my goal of helping everyone understand the importance of using analytics to make decisions on content...marketing and content teams for their help and support throughout the past six months!...

...doing a postdoc few science trainees will have opportunities to work on Emotional Intelligence or to hone...sense of engagement in the work Behind-the-scenes support from their bosses and employers. Availability of...in the goals of the organization and feel the importance of their contributions. Flexible work schedule...of each team member) Need challenging work, opportunities to pursue and problems to solve (I think this...self-directed, but need precise leadership and support from their superiors (beware of not micromanaging...highly mobile and can move to a new workplace if opportunities for learning and personal growth do not exist...

...You've heard it before – it's important for scientists to get out there and talk about their work. To... is cool and why it really matters! There are important societal reasons, self-interested reasons, and...collaborative roles in academia, and that means it’s important to know how to talk about science to everyone ...-doc start? The good news is there are more opportunities than ever before for scientists to communicate...Local science museums are a great place to find opportunities for science outreach. Here in the North Carolina...institutional news office if there might be opportunities to blog or write for them. 10)  If you are serious...

...enrich and select for cells that had successfully incorporated the desired mutation, and then derive mice from...handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant females, a technique...gRNA, Cas9, and donor oligonucleotide can be electroporated into the mouse zygote (Qin et al., 2015).  ... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... microinjectionist, who has the strategically important job of delivering the payload! Last but not least...-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease. Genetics 200:423–430 . https:/... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated...

...Cpf1. This tool supports over 250 genomes, and they just released a brand new CRISPOR manual to kick off...evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise, the NIST Genome...Genome Editing Other resources and publications CRISPOR gRNA design tool Researchers at the Keystone meeting...meeting had great things to say about CRISPOR, a tool to help you design, pick, and close guides for Cas9...strand annealing (SDSA) and single-strand DNA incorporation (ssDI). "Easi-CRISPR for creating knock-in and...

...mentoring support we need, and most of us could use more mentors to listen, guide and support us during...mentors don’t need to be more senior or more “important”, they just need to know you well enough to ask...initiate things independently” and “the power or opportunity to act or take charge before others do”. Candidates... State University; Graduate Student Funding Opportunities Open Education Database: 100 Places to Find ... Florida: Training Grants and Other Funding Opportunities L’oreal USA For Women In Science If you have...

...also take negative data, which is equally important to report. We recently published a paper in Frontiers...did you use? For animal subjects it is helpful to report the genetic background and catalog number or RRID...designing your experiment, read our blog post on important considerations when using AAVs. Experimental results...approval from the copyright owner and will have the opportunity to cite the image to give proper attribution.... hub! Related resources on the Addgene blog Important Considerations When Using AAVs Three Key Considerations...

...machines could potentially be used to report upon and/or engineer “temporally ordered biochemical events” such...Lab at MIT developed plasmid based systems that report the order in which specific chemical inputs are...derived from Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to...viral replication and transcription as well as reporter genes. To learn how you can use minigenomes in...School describes how AAVs function and indicates important things to consider if you’re getting started with...

...research studies highlight the importance of taking a break and incorporating EAs into your routine.  Getting...other options. While graduate programs provide support for academic career development, it's often left...unproductive time spent away from the bench, but as an opportunity to expand associations outside of the traditional...on improving and will help reveal potential opportunities beyond academia. Once a profession sounds interesting... EAs allow graduate students and postdocs an opportunity to tailor their career paths for competitive ...

... phenomenon? Exploration can be important, but it is equally important to have a plan to get to mechanism...are your support network, so remember to thank them verbally and often for their love and support. Many...from long periods of training to limited job opportunities in academia - the main reason most people enter...outcome, e.g. where could you publish this and how important is the result? Is there a tractable question ... better writer. First, practice often. Seek opportunities to write manuscript drafts, reviews, cover letters...

...to scientists and open your eyes to the many opportunities available to those who are scientifically minded...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...job title actually involves, other than what is portrayed in the media and in films, which can often be ...skills? Other things to consider are wage, opportunity to advance, and how many jobs are normally available...

...AAV plasmids contain several important elements, but among the most important are the inverted terminal ...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene...modifications and types  Since ITRs are one of the most important elements of AAV and rAAV vectors, modifications...site reduce Rep nicking. Since Rep proteins are important for viral genome replication, these mutations ...is still limited, but this area of research is important for downstream AAV applications.  Tips & FAQs ...

...go about starting a lab? Obviously, funding and support are crucial parameters, and familiarity with equipment...a PhD student in his first year. “It was very important to me to be with them in the lab when they started...spending more time in his office, he gradually incorporated regular group meetings, journal clubs, and focused...the lab and to take things slowly! It is more important to carefully select first hires who are well-suited...seek out advice from seasoned PIs who can offer support. “It is an exciting and emotional time!” George...a challenging process. Each PI emphasizes the importance of slowly building up your lab and selectively...

...individual cells. Electroporation on the other hand uses electrical pulses to create transient pores in the cell...plasmid of interest. Scientists can also use electroporation, the application of an electrical charge to...lysogenic cycle, the phage’s genetic material is incorporated into the host’s genome at a particular integration... and stable cell lines, where foreign DNA is incorporated into the cell’s genome and is thus passed down...charged cell membrane. Physical methods such as electroporation or microinjection actually pokes holes in the...