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Showing: 241 - 260 of 502 results

  1. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...PubMed user searches, such as clicks to read the abstract or full text of the paper, publication date, a...publications and figures using machine learning to extract important features such as influential citations...

  2. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...replaces the targeted gene and provides a way to track successfully engineered cells. Similar to transgenic... the cell can occur. Similar systems include tetracycline or doxycycline mediated activation of Cre to...

  5. Design Tips for Prime Editing

    Type
    Blog Post
    ...near point mutations to create 3-base (or longer) tracts of edited bases. DNA mismatch repair (MMR) is less... protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) can improve...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...and requires only one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... produce low levels of virus and can be used to track carryover. Functional titer Functional titer is ...Learn How Lentivirus Has Been Used for Lineage Tracing Additional Resources on Addgene.org Learn How...

  11. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...our ideas got pretty good. We started doing extracurricular research on some of them to see how feasible...SynBio Axlr8r We limped along with very little traction for quite a while, and even had some serious self-confidence...

  13. Transferable Skills: Negotiation

    Type
    Blog Post
    ...business suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The mythical... committees as a tenured professor or signing contracts when working for a business development team, ...

  14. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent 0.001%...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

  15. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  19. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...sequences (blastn)” program for blastn so that you can extract practically every single reliable base pair from...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  20. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...1993). But why did this technique fail to gain traction? It is likely that both vector amplification with...performing multiple modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific...
Showing: 241 - 260 of 502 results