...methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "Direct visualization of newly... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

...level of antibodies is reached - typically within 2-4 months. After the animal has the desired level of ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

... phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, R6K...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

...Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-66. PMID: 17888184; PMCID: PMC2075523. Bulcha, J.T...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

...Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL ...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch...

...PubMed PMID: 1495975. PubMed Central PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

...activation and base editing applications.  Figure 4: Overview of CasMINI for genome engineering applications... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...

...in short term storage (think days to weeks) in the 4°C. If you’ll only be using it every few month or if...fluorophore for an experiment is easy, but what about those times you need more than one?   Multiplex ...

... Wang et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84... Park et al., Science 2011 Aug 26;333(6046):1151-4. pOSIP and the Clonetegration Classic genetic ...assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces the number of...2012 Mar 20;109(12):E690-7. For further reading about SpyLigase technology, read the Addgene interview...; DOI: 10.1021/sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research...in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN...pGreen-IIS based destination vectors. To learn more about the GreenGate cloning system, see the detailed plasmid...

...notebook, opened it, found my outline, read over Figure 4, took a deep breath, and, re-oriented, continued presenting...silence, I opened my mouth to panic-babble something about Western blots that made no sense, even in my head...doubt anyone but me remembers any of the incidents above–and the dumpster noises were just last week!  Nerves...attention? fear of messing up?–and use the three tips above to make a strategy that can help. Even something...speaking as a transferable skill The worst thing about doing public speaking as a scientist is that you...perfectly, you truly are a rock star.  The best thing about public speaking as a scientist is that you will ...increases visibility of your work, opens up collaboration and growth opportunities, and gives your entire...

...ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding affinity... time, when someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD...mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-crystalize...for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two Fab fragments...2019). Structural diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans...

...Always ask for references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will...Individual Development Plan Learn a Little about The Academic Labor Environment from the Future of Research...research.  It helps to spend some time thinking about what your goals are for the coaching relationship...with someone you connect with. Talk to the coach about their “style” and make sure this is a style that...coaching group is Still Point Coaching. One nice thing about a group is that sometimes this offers the option...

...real-time PCR experiments. Clin Chem. 2009 Apr;55(4):611-22. doi: 10.1373/clinchem.2008.112797. Epub 2009...GAPDH. Biochem Biophys Res Commun. 2001 Mar 9;281(4):951-6. doi: 10.1006/bbrc.2001.4439. PMID: 11237753... should have a melting temperature 5-10 degrees above the primer and be at least 25 bp away from each ...

...organizations to see their news and job postings. 4) Use LinkedIn to pay it forward The best way to network...include a note about where you met IRL (in real life) and perhaps what you talked about (or how good the... this rule, but not that many). I’m asked a lot about requesting connections to complete strangers for...have been able to tell when some start-ups were about to be bought by seeing certain groups of people ...using social media as a scientists Read blog posts about networking ...

...cardiomyopathy. An adult male with a heterozygous 4 bp deletion in MYBPC3 donated skin, blood and semen...August 2nd in Nature gives us even more to talk about, as it represents another step towards CRISPR germline...impossible with current tools, thus quieting some fears about the creation of “designer babies.” The differences...of individuals with varied backgrounds. Talking about this study with non-biologist friends Like me, you... Blog Check out Our New CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes...

...to visualize low-abundance proteins. Figure 4: Overview of using Indirect ELISA to quantitatively...blog Learn about the advantages and disadvantages of using monoclonal antibodies. Read about other techniques... require two different antibodies. As mentioned above, it can also be used to characterize antibodies ...concentration of proteins in an ELISA well As mentioned above, the measurement of the concentration of proteins...

...consists of 20 plasmids encoding 14 alpha, 2 beta, and 4 gamma subunits of the heterotrimeric G protein complex...chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime have... inhibit neurons. You can find information more about chemogenetics and a concise table of DREADDS and...Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics Resources...

...receptor for adeno-associated virus infection."Nature 4:520(7588):108-12 (2016). PubMed PMID: 26814968. McCarty...make AAV ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression...genetic cargo. Typically, when researchers speak about AAVs, they are actually referring to rAAVs. ITRs...Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org ...

...light touch in mechanical allodynia. Figure 4: While Qiu’s work on the PV neurons is in progress...students as we have done in the past. Below, read about the five winners and their research.   Abhi Aggarwal...work has only been made possible due to the collaborative and highly interactive environment that Janelia...biosensors or biosensors that detect new redox metabolites are vital for researchers to better understand...

...PMID: 18614669. PubMed Central PMCID: PMC2593125. 4. Saunders A, Johnson CA, Sabatini BL. 2012. Novel ... Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector ...12665802. 2. Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases...