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We narrowed to 954 results for: app

Showing: 391 - 420 of 954 results

  1. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...favorite FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if...got a useful tool, there’s likely someway you can apply it in neuroscience. AAV tools from Addgene are already...targeting genes to specific cell types in the brain, mapping neuronal networks, and selectively activating signaling...

  2. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... are here to discuss the ins and outs of both approaches. Screens and selections The common goals The...lot in common. In fact, selections are often inappropriately categorized as screens! Why the confusion? ...details - and that’s where the differences in these approaches can be found. As an example, say you are considering...require there to be a culture condition that can apply enough pressure to select for variants of interest...

  3. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... with an intuitive user interface.   The team approached the issue from multiple angles: the overall user...of information so we were able to fine tune our approach much more than if we were dealing with general...” variations and synonyms of the same term that appear in our data into a single filter value. For example...our search engine to be so specialized that it appears to “understand” biology.  We hope you will give...

  4. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...review system. Peer review in traditional journals happens before the manuscript is accepted for publication...at the same time. Take your time picking the appropriate journal and explain in your cover letter why ...why you think your manuscript is appropriate for that journal. Argue with an editor or reviewer over their...ignored their comments, they will not be inclined to approve your paper. Rush your submission or revision. A...

  5. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...with the same genetic background and do an apples-to-apples comparison of all those mutations. If you ...take four plasmids out of the library in the appropriate combination and do a restriction enzyme digest...different genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare...

  6. Summer SciComm: Preprints

    Type
    Blog Post
    ...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)...and preprints are a great way to do that. We are happy to receive deposits of plasmids that have been described...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

  7. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...researchers to better understand the processes that happen inside the cell,” says Pedre Pérez. Figure...construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due...

  8. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...systematically mutated it. Using a robotic picking approach to select cells with variant characteristics they...” vector cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest ...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...

  9. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...previous post in this series described a practical approach to selecting a bright fluorescent protein. In ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets in a water/oil emulsion, (ii) ...

  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...several methodologies currently available for approaching it. These include the traditional restriction...introduction.html demonstrating the principal behind the “swapping out” of ccdB with the investigator’s gene of interest...which can then be used for downstream cloning applications. While these two strains ultimately perform ...important to ensure that you choose the most appropriate method for your own experiments. Please check...

  11. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...high-throughput screening are vastly useful for mapping the combinatorial genetics that underlie complex...research questions. CombiGEM has been successfully applied to functionally characterize combinatorial gene...realization of a variety of perturbations and applications in functional genomics, cell reprogramming, ...He is particularly interested in developing and applying new technologies to interrogate and understand...

  12. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...differently – happens when the antibody is produced from a new set of animals), and wrong application (different...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...

  13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...need to be given some time to adapt to a testing apparatus. General stress levels of the test animal may ...the humans’ audible spectrum. The experimental apparatus should be cleaned thoroughly between animals as...excitation or inhibition upon light (or drug) application.  Genetic manipulation can also reduce expression...after injection. Therefore, it’s crucial to appropriately time the injection about two weeks before the...

  14. A Look at Addgene's QC Process

    Type
    Blog Post
    ...mutated; elements that are difficult to sequence; approaches to confirm plasmid sequences without a reference...program called Geneious. Here, the raw reads are mapped against a reference sequence, if one was provided... determine if there are any notable QC issues. Mapping the raw NGS reads to a reference sequence can also...confirm their presence with confidence. If this approach does not resolve the identified differences, the...

  15. Engaging with science and society at pgEd

    Type
    Blog Post
    ...these tough issues is essential for building a rapport with these communities, which is in turn crucial...latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...scientist. After all, scientific research does not happen in a vacuum that is isolated from the rest of society...and who will be affected by the knowledge and applications generated from this research. At both a practical...

  16. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high...AdVs is what makes them ideal candidates for applications in oncolysis and vaccination!) In the 65 years...Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new...

  17. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...to increased intracellular calcium. It has been applied to mark active populations of neurons in vivo to...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction to AAV ...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... the end goal, it is best to work with FDA/EMA approved drugs so that your results are viable for translation... be mitigated by using low doses and running appropriate controls (Jendryka et al., 2019), they have resulted...brain and some, such as varenicline, are already approved for clinical use. Varenicline has been further...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

  19. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...organizers and moderator should have a well-planned approach to making the event interesting and engaging. ...information and remind them about the event as it approaches. Room set-up and logistics To make a really...and forth within the panel. Go with it when this happens, but get things back on track if the direction ...talk and the speaker goes over 1 hour, watch what happens to the attention of the audience – phones come ...

  20. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...have gotten a lot of press for being a promising approach for treatment or prophylaxis of COVID-19. Researchers...nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses...BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, ...single domain antibodies for the conformational trapping of membrane proteins. eLife 7: . https://doi.org...

  21. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...working with mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus...have capacities of 8–12 kb, while AAV only has approximately 4.7 kb — which means that when using Cas9, you... Platforming AAV Interest in interrogating and mapping the genetic landscape of tissue-specific diseases...Performing In Vivo CRISPR Screens Using the FITS Approach Additional resources at addgene.org CRISPR pooled...

  22. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... experimental, biotechnological, and medical applications. Researches can visualize proteins in vivo by...protein you need   Your plans for downstream applications In this blog post we will summarize some of...proteins as bacterial cells are not equipped to add appropriate post-translational modifications. In addition...acids that are useful in different downstream applications. Cell-free expression systems however, are expensive...

  23. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...order to correct for this, compensation has to be applied to “subtract” this spillover and correctly distinguish...designing your panel. If you have two highly overlapping cellular markers, it is important to pair them...have a similarity index of 0.87; therefore, two overlapping cellular markers should not be paired with these...index of 0, which would be a better choice for overlapping markers. Compensation/reference control(s) Compensation...

  24. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... per year, or approximately 104 plasmids shipped per working day, including appropriate MTAs and customs...of CRISPR tools has grown steadily, gaining approximately 2,000 plasmids/year from 2017 to 2023. This ... joining the Addgene CRISPR community, with approximately 100 new CRISPR depositors each year.    ...

  25. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...months, and we got one submission. I was a bit disappointed in the lack of participation, but I decided ... passed these dinosaurs every day. They made me happy. Grad school can provide plenty of negativity, so... so I volunteered at a place that made me happy. I started volunteering in the “Dinosphere” almost every...was worried about becoming one of those angry, disappointed graduate students because I wasn’t sure what...

  26. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ... and have been adapted for a wide variety of applications - as mutagenesis tools for genetic studies (...Recently, the lab introduced a streamlined expression approach (Fig. 2A), involving two-plasmid or single-plasmid...at one or multiple desired genomic loci. Many applications of these systems have already been demonstrated...C , Xu M, Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, ...

  27. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... critical for a number of popular scientific applications, and the quality of a standard curve can make... then generate a trendline for the data. The appropriate trendline varies between assays and may be linear...for an acceptable R2 but for many scientific applications users aim for an R2 above 0.95.    Additional... from decreased specificity, especially as it approaches the minimum and maximum thresholds. If the measurements...

  28. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...cells stay in an HDR-promoted cycle longer. This approach may have some negative outcomes on viability if... as 10 hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that...facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is a Cas family...knock your knock-in experiment out of the park. Happy editing!   Resources and References References...

  29. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...down to the exact plasmid you need. The ability to apply a wide range of filters isn’t new to Addgene’s plasmid... it’s also a convenient most requested filter. Applying a second filter, this one from the ‘Requests’ ...consider. If you get this far and realize you need to apply different filters you do not need to start a new...” plasmids returns more than 1300 results, and applying a filter for “CRISPR” plasmids keeps more than...

  30. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...activity. In this study scientists examined approximately 1,900 sgRNAs and identified sequence features...substantially identified more hits than the SAM library approach in a comparison study screening for vemurafenib...ORF overexpression library identified several overlapping genes, most genes were unique to the individual...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...
Showing: 391 - 420 of 954 results