We narrowed to 809 results for: MAK

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...receiver cells. There are over 40 trillion cells that make up the human body and G-baToN provides versibility...

...safety profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are...homology directed repair (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated...

...transfer office or other authorized office directly to make sure the deposit agreement/MTA is taken care of ...spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries with...

...low-cost, field-based diagnostics, our approach could make an impact. Our motivation was underscored by the... switches and amplification primers. This helped make sure that we had a number of successful sensors ...

...cell membrane. His recent work shows that they are making fairly remarkable progress on establishing a system...the drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics. These...

...separates the two components of the indicator, which can make the kinetics of voltage sensing to signal output... (voltage sensing) hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for ...

... humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...purified. Although a ~1% change in signal might not make a very powerful tool, it was promising enough to...

...additional datasets used in the training of PRIDICT2.0 make this model more suited for prediction of very diverse...includes applying machine learning techniques to make sense of biology, with a special focus on gene editing...

...chromatin interactions, they are grainy so it’s hard to make out the details. To increase the resolution, the...genomic loci of interest. This is cumbersome and also makes it hard to compare results across multiple targets...

...chromoprotein detection can be done by the naked eye. This makes cloning quite a bit easier since you can see if ...Learn more about FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find...

...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

... inadequate plasmid DNA yields. Why would volume make a difference? In the simplest of terms, more volume...cells present in the given volume.  Let oxygen in! Make sure your culture is getting the right amount of...

... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant...and mCherry). These simple strategies can help you make better fluorescent fusion protein reporters for ...

... learned along the way and hope they’ll help you make the most of the opportunities you’re provided throughout...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest...

...made publicly available through Addgene.  What makes CRISPR so special? A major advantage of CRISPR/Cas9...  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...

...developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes to ...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens...

...accurate, as these peptides are thought to function by making the ribosome skip the synthesis of a peptide bond...within the same multicistronic vector, effectively making a construct that expresses multiple unique genes...

...or validating your new clones, try these tips to make the most of your time and sequence! You might also...Standard Nucleotide BLAST page, the first decision to make is whether to compare a Sanger sequencing result...

...are of no use to you. When using more than one FP, make sure their emission light does not overlap in wavelength...eel was shown to mature independently of oxygen, making suitble for use in anaerobic conditions. Maturation...

... a specific field. While searching for jobs and making connections, avoid falling into an identity trap...yourself, and to demonstrate them to the people who make hiring decisions. Though having all the technical...

...powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in glass milk, ...but the Zhao and Deng labs found they could just make their own buffers when the kits buffers ran out....

...our samples 1:6 million to 1:25 million.  After making the dilutions, they are transferred to a new plate...generator, you should avoid pipetting too quickly when making your virus dilutions. This will reduce aerosols...

...spectrophotometry. Microvolume spectrophotometers make it quick and easy to measure sample concentration...absorbance strength is low compared to other reagents, making it more difficult to detect. A particularly strong...

...has greatly enhanced the ability of scientists to make genomic alterations, bringing about a revolution...the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications where limited...

...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S...

...tumors. Lentiviral vectors are also commonly used to make stable transgene-expressing or knockout cell lines...clinical stages. Researchers are also working to make lentiviral vectors safer. Since lentiviral vectors...

...resources are being spent to make something the cell doesn’t need to make. Eukaryotes and some bacteria...

...excited because genome-editing with these molecules makes it much easier to knock-in LexA binding elements...developing several additional systems of enChIP to make the technology more user-friendly and precise. We...

...than age-matched fibroblasts [3]. These advantages make PBMCs the ideal source of somatic cells for reprogramming...virus method is expensive, especially if used to make clinical grade hiPSCs. For example, the CytoTune...

...standard curve of known concentration. We regularly make and validate fresh plasmid standards. We validate...determine accuracy and reliability. When doing this, we make a judgement call on whether the two titer values...

...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ...and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...

...buffer components can interfere with conjugates. Make sure to do your research before conjugating at the...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...

...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...effective reporter for adenine base editors, that make A-to-G edits in the genome (Gaudelli et al., 2017...

...edits but the double strand breaks it introduces makes it difficult to perform two or more integrations...targeting another site of interest can be used to make other edits. Once you have all the edits you want...

... expression in a cell type-specific manner also makes them useful tools for targeting those specific cell...enhancer candidates. To use these sequences for making functional genetic tools, enhancers were nominated...

...the list of antibiotic options doesn’t end there. Make sure you read the instructions for whatever antibiotic...kingdoms of life. A bit of careful design will help make your experiment with shuttle vectors a success! ...

...as well as high spatial and temporal resolution, making it ideal for visualizing gene expression in vivo...They also did not scatter ultrasound nonlinearly, making them difficult to distinguish from background tissue...

...for whom English is a second or third language (making publishing their research all the more challenging...Washington in St. Louis, offer guidance on how to make a low-cost whiteboard video about your research....

.... Consultants often work with such teams to help make decisions about R&D investments in either new internal...benefits of novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running...