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We narrowed to 801 results for: MAK

Showing: 521 - 560 of 801 results

  1. CRISPR in the Clinic

    Type
    Blog Post
    ...effectiveness, if the editing frequency isn’t high enough to make a physiological difference, then the treatment will...systems as well as improving delivery strategies to make fast, effective, and targeted therapy possible. ...

  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...low-cost, field-based diagnostics, our approach could make an impact. Our motivation was underscored by the... switches and amplification primers. This helped make sure that we had a number of successful sensors ...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...purified. Although a ~1% change in signal might not make a very powerful tool, it was promising enough to...

  5. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...additional datasets used in the training of PRIDICT2.0 make this model more suited for prediction of very diverse...includes applying machine learning techniques to make sense of biology, with a special focus on gene editing...

  6. Of Myc and Men

    Type
    Blog Post
    ...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...separates the two components of the indicator, which can make the kinetics of voltage sensing to signal output... (voltage sensing) hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for ...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... inadequate plasmid DNA yields. Why would volume make a difference? In the simplest of terms, more volume...cells present in the given volume.  Let oxygen in! Make sure your culture is getting the right amount of...

  12. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... learned along the way and hope they’ll help you make the most of the opportunities you’re provided throughout...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest...

  13. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...made publicly available through Addgene.  What makes CRISPR so special? A major advantage of CRISPR/Cas9...  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...

  14. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes to ...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens...

  15. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...accurate, as these peptides are thought to function by making the ribosome skip the synthesis of a peptide bond...within the same multicistronic vector, effectively making a construct that expresses multiple unique genes...

  16. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...or validating your new clones, try these tips to make the most of your time and sequence! You might also...Standard Nucleotide BLAST page, the first decision to make is whether to compare a Sanger sequencing result...

  17. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...are of no use to you. When using more than one FP, make sure their emission light does not overlap in wavelength...eel was shown to mature independently of oxygen, making suitble for use in anaerobic conditions. Maturation...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    ...powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in glass milk, ...but the Zhao and Deng labs found they could just make their own buffers when the kits buffers ran out....

  20. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...our samples 1:6 million to 1:25 million.  After making the dilutions, they are transferred to a new plate...generator, you should avoid pipetting too quickly when making your virus dilutions. This will reduce aerosols...

  21. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...has greatly enhanced the ability of scientists to make genomic alterations, bringing about a revolution...the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications where limited...

  22. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S...

  23. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...tumors. Lentiviral vectors are also commonly used to make stable transgene-expressing or knockout cell lines...clinical stages. Researchers are also working to make lentiviral vectors safer. Since lentiviral vectors...

  24. Plasmids 101: Protein tags

    Type
    Blog Post
    ...resources are being spent to make something the cell doesn’t need to make. Eukaryotes and some bacteria...

  26. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...the list of antibiotic options doesn’t end there. Make sure you read the instructions for whatever antibiotic...kingdoms of life. A bit of careful design will help make your experiment with shuttle vectors a success! ...

  27. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...buffer components can interfere with conjugates. Make sure to do your research before conjugating at the...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...

  29. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...for whom English is a second or third language (making publishing their research all the more challenging...Washington in St. Louis, offer guidance on how to make a low-cost whiteboard video about your research....

  32. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...standard curve of known concentration. We regularly make and validate fresh plasmid standards. We validate...determine accuracy and reliability. When doing this, we make a judgement call on whether the two titer values...

  33. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...edits but the double strand breaks it introduces makes it difficult to perform two or more integrations...targeting another site of interest can be used to make other edits. Once you have all the edits you want...

  35. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

  36. Hot Plasmids - November 2023

    Type
    Blog Post
    ...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ...and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...

  37. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within living...approaches and appropriate controls are required to make this method useful for dynamic experiments in which...

  38. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the...on type of RNA transcript, you will also need to make sure your plasmid has a promoter suited to working...

  39. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

  40. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...express multiple integrins that bind the same ligand, making it difficult to dissect the function of one integrin...protein research institute, partnered with Addgene to make our collection of integrin antibodies available ...
Showing: 521 - 560 of 801 results