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  2. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and...

  5. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI.   Additional...resources Find protocol videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ...

  6. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production...

  8. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ... where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it ...understanding the minimal genome. Many of these techniques require more complex DNA assemblies and improvements...

  9. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular...research focuses on a range of topics including biotech and research tools, computing performance, and ...

  10. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated...

  14. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These...

  15. Hot Plasmids - January 2023

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    Blog Post
    ...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9... for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed of light over the...

  18. 5 Great Apps for Lab Life

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    Blog Post
    ...Culture by Gibco Whenever I start to learn a new technique, I try to look for a resource to help me. I’m .... When I started learning proper cell culture technique, I tried to find an app that could help me with...

  19. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...thought that they function as a kind of armor to protect their host from predation (Petroni et al 2000)....the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest ...

  20. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post
    ...fluorescent proteins whose spectra overlap, there are techniques, like spectral unmixing, which can be used to...green and red proteins are generally the most detectable and so should be used to tag your least abundant...

  21. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...indicator of transcription within cells, where detection of the reporter protein or its enzymatic activity...regions on gene expression can be determined by detection of the reporter in a specific assay, which ideally...

  22. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection...

  26. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...educational materials, and provides viral vector technical service (details about virus production protocols...

  27. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...

  28. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>...

  29. Clear and Accessible Writing

    Type
    Blog Post
    ...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

  30. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating any other ...Others have noted a lack of transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe...

  32. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The other component...Addgene Blog Keep up with the Latest Cloning Techniques Learn to Troubleshoot Your Sequencing Results...

  34. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...overview of general molecular biology knowledge and techniques. If you are interested in reading more, you can...Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated list of Bacterial...

  35. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post
    ...the steps you will have to take. Decide which technique you are going to adopt (i.e. restriction cloning...Find how-to videos for molecular biology and lab techniques ...

  37. dTAG - You're it!

    Type
    Blog Post
    ...exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective small molecule...author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet.   References...

  38. RNA Extraction Without A Kit

    Type
    Blog Post
    ...laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions...

  39. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... will not recognize a Fab fragment. In order to detect a Fab fragment, it either needs to be directly ...identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem Cytochem...

  40. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... the open windows in the middle of your talk. Technical difficulties turn your slide deck into a chalk...everyone! Everybody makes mistakes, encounters technical difficulties, and runs into unexpected stumbling...
Showing: 521 - 560 of 797 results