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  1. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...he and his colleagues at Duke University, in collaboration with “cloning guru” Kathleen Ottina and David...it cloning by mail,” Wood says, and it took them about six months from start to finish. They cloned all...

  4. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H...

  6. Tag Your Favorite Yeast Genes with Ease

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    Blog Post
    ...strain. In addition to the collections featured above, many other modular yeast tagging systems have been...Addgene would love to hear from you, our community, about your experience with yeast genome modifications....

  7. Supporting Rare Disease Research with BeHeard 2017

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    Blog Post
    ...We often hear about breakthroughs in diabetes and lymphoma research or the many different ways you can...little-known disease. You can find more information about the disease on the Meothelioma Cancer Alliance Website...

  8. Working 9-5: an Addgene Co-op

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    Blog Post
    ...intimidating to me, as I had a hard time thinking about how I could fill this time. I was relieved to find...attend to other commitments, without feeling guilty about missing work. As long as my work was finished on...

  13. Sensing Neuronal Dopamine

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    Blog Post
    ...making, and motor control.  What do we understand about Dopamine? DA is produced by specific dopaminergic...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  18. Plasmids 101: What is a plasmid?

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    Blog Post
    ...Molecular Biology Reference includes information about molecular cloning, how to choose a plasmid vector...straightforward as possible. If you have a question about a specific plasmid element that you would like answered...

  21. Antibodies 101: Fc Effector Functions

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    Blog Post
    ...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

  22. Build Your CRISPR Vocabulary

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    Blog Post
    ...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

  23. Running for Rare Disease, Running for FOP, Running for AJ

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    Blog Post
    ...on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based ...Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers...

  24. Management for Scientists: What Makes a Good Manager Anyway?

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    Blog Post
    ...the same as not being a bad manager (see cartoon above – what type of bad manager have you had?). You can...Managing scientists I should add a few special words about managing scientists because, let’s face it, we are...

  25. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  28. Using Addgene's New Viral Service for Your Research

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    Blog Post
    ...us with AAV testing and recently had this to say about the service: "I'm excited that Addgene is now...other expression data. If you have any questions about the Viral Service or any of the materials we distribute...

  29. Plasmids 101: Origin of Replication

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    Blog Post
    ...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

  30. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    Blog Post
    ...Our work accelerates and enhances productive collaborations as well as provides visibility for grant-driven...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...

  33. RUBY-Red Siliques

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    Blog Post
    ... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-red: You can’t beet it!) About the Author  Andrew Willoughby is a graduate student...

  34. Antibodies 101: Epitope Availability

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    Blog Post
    ...naturally.  Sample Denaturing  First, let’s think about induced conformation changes. Several types of assays...immunoimaging assays. It can also elucidate information about what a given protein is doing or changing in different...

  35. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ... using biorender.com   If you’re new to talking about viruses, here’s a quick tip on terminology. “Virus...have a growing number of Viral Vectors 101 posts about each type, so be sure to check our blog, or our ...

  36. Antibodies 101: Epitope Tags

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    Blog Post
    ...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

  38. Plasmid Cloning by PCR

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    Blog Post
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time...Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things ...

  39. Mentoring for Scientists: I Have a Mentor, What Now?

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    Blog Post
    ...getting together and chatting with another person about their career path is a great start to a mentoring...Medical School researcher Connie Cepko to learn about her mentoring style. Set goals with a mentor: The...

  41. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...brightness after ~100 action potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  43. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 37825  AAVrg...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  45. Science Career Options

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    Blog Post
    ...skills and experience.You might not normally think about it, but hobbies and volunteer experiences, as well...show that you have some of the abilities listed above. Showing that you are willing to exercise these ...

  46. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...conduct the screen. To help alleviate some of this labor for our users, we now provide the Brunello (targeting... hear from you if you have used or are thinking about using our viral services. Please send any questions...

  47. Plasmids 101: Gram Negative and Positive Bacteria

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    Blog Post
    ... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

  49. The MTA at Addgene

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    Blog Post
    ... who aren’t familiar with it. If you’re curious about the hows and whys of the material transfer agreement...easier for others.  If you’ve still got questions about our MTA process, you can check out our Tech Transfer...

  50. Management for Scientists: Delegating is Key

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    Blog Post
    ...Medical School researcher Connie Cepko to learn about her management style. Recipes for delegation success...accountability? What types of issues do you want to know about right away? Provide adequate support. The manager...
Showing: 651 - 700 of 962 results