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TypeCollection...embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light chains. Antibodies are...
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Distribution to Industry
TypeCollection...between the recipient and the depositing institution(s). It governs the transfer of biological materials ... -
CRISPR 101: Off-Target Effects
TypeBlog Post...involves making specific knock-out edits in a gene(s) of interest and has an average off-target frequency...CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) CRISPR 101: Validating Your Genome Edit ... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d Ref...239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared... -
Five Popular Model Organisms, Part 2
TypeBlog Post...://doi.org/10.7554/elife.06100 Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings... -
Neurodegeneration Plasmid Collection
TypeCollection...TDP43 RRM-GFP sigma-S TARDBP CMV ALS Rajat Rohatgi 107799 pHBS931 TDP43 RRM-GFP F-S TARDBP CMV ALS Rajat...TDP43 RRM-GFP FYW-S TARDBP CMV ALS Rajat Rohatgi 118792 pHBS1385 TDP43 RRM-GFP VLIM-S TARDBP CMV ALS Rajat...pHBS1410 GFP-TDP43 F-S TARDBP CMV ALS Rajat Rohatgi 118800 pHBS1411 GFP-TDP43 FYW-S TARDBP CMV ALS Rajat...BFP-P2A-TDP43 F-S] TARDBP CMV ALS Rajat Rohatgi 118809 pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S] TARDBP...SUMO-TDP43 F-S-TEV-mCherry TARDBP T5 ALS Rajat Rohatgi 133322 pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherry...-TDP43 F-S] TARDBP CMV ALS Rajat Rohatgi 133329 pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S] TARDBP ...Rajat Rohatgi 107800 pHBS935 TDP43 RRM-GFP M-S TARDBP CMV ALS Rajat Rohatgi 107801 pHBS934 TDP43 RRM-GFP... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...orthogonal nuclease-inactive Cas9's (dCas9) from S. pyogenes, N. meningitidis and S. thermophilus which have been...favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112...620nm) portions of the spectrum. PhD student Benjamin S. Padman recently developed a mitochondrially-targeted...context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for... -
Hot Plasmids: Spring 2025
TypeBlog Post...Hiraizumi, M., … Abudayyeh, O. O., & Gootenberg, J. S. (2025). Reprogramming site-specific retrotransposon...Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. L., Perrimon, N., & Ting, A. Y. (... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...topics. Resources and references References Aydin S (2015) A short history, principles, and types of ELISA...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post.... Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing...plasmids from this publication at Addgene. Stolzenburg S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan... -
Antibodies 101: Validation
TypeBlog Post...like Antibodypedia or through your antibody provider(s) if they accept and share user data on the antibody...10.1016/j.nbt.2018.08.002 Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...lately (1, 2), depends on knowledge of the structure(s) a given phage will recognize on a host cell. To ..., Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...as effective at editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (...health. References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini... -
Easing the Protein Purification Process with pCri
TypeBlog Post.... Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification... -
Lentiviral Prep Service
TypeCollection...Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance ... -
Genetic Code Expansion
TypeCollection...209744 pAB228v TrpRS S. cerevisiae Bacterial Ahmed Badran 209745 pAB228v6 TrpRS S. cerevisiae Bacterial...Badran 209746 pAMC070n1a12 ScTrpRS, Lum1PylRS, MmPylRS S. cerevisiae, Lum1, M. mazei Bacterial Ahmed Badran... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post... al also used arg1 to in vivo image tumour-homing S. typhimurium strain ELH1301. Bourdaeu et al see this... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post..., Addgene has “literally enabled sharing with 100's of investigators worldwide. Now everything from every... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...photoconvertible proteins. With Sattarzadeh et al.’s discovery that GFP itself is photoconvertible, new... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...efficiency of BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva N, King K, Davis JR, Mullins...