We narrowed to 730 results for: ACE;
-
TypeBlog Post...depends on a surface glycoprotein – the only viral protein present on the virion surface – making it critical... system in a novel way, by shielding host cell surface proteins. Researchers at the University of Virginia...
-
Designer PUF Proteins for Any RNA Target
TypeBlog Post...factors – to specific sequences of DNA has become commonplace. This ability has opened up a world of possibilities...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...tips: First, she recommends using standard BsaI in place of the high fidelity version (despite what the original... -
Why Do I Need an MTA Anyway?
TypeBlog Post...transfers. When Addgene entered the plasmid sharing space, we heard over and over again that the legal documents...experiments with confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform... Recipients: Streamlined Processing: When you place an order, Addgene generates the MTA and emails it... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...relatively the same for quite a while and hadn’t kept pace with our growth over the last few years. Some of... professional, and modern. Instead of trying to place all of the content above the fold, we decluttered...elements and allowed our content to dictate the space. We wanted to comfortably tell our story, to get... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog Post...perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration of tangents...ideas and approaches. Moreover, the ability to embrace serendipity fosters adaptability, a crucial skill...your academic journey, remember to leave a little space for the unexpected — it might just be the key to... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post... a double-strand break, and when used with two adjacent gRNAs, can lower the probability of off-target... of the targeted region, whereas PAM-in designs place the PAMs closer together in the middle of the targeted...sites separated by 51-68 bp Use D10A for HDR Place intended insert in between nick sites Use 40 nt... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post... free online molecular biology tools all in one place. Plasmid mapping These tools are for viewing, editing... looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for...Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence, making it ready... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...your workplace and you could end up making a huge impact on how waste is handled. A lot of places have...on waste, but also on culture. Encourage your workplace to become a leader in sustainability. Sign up... -
Tips for Improving Your Next Manuscript
TypeBlog Post... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site courses... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...continue to help bring HOPE to AJ. PS: The superhero race is the perfect event for supporting AJ, who is a...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post... amazing job shuttling unwanted lab supplies to places that really need them. When I was in graduate ...indefinitely at room temperature (thus also saving freezer space). The procedure becomes less attractive when noting...very thin gel on a large glass slide using only surface tension. It's a bit tricky to pour the right amount... -
Choosing a Good Mentor for Scientists
TypeBlog Post... and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting Tenure...thing to do is not go into their labs in the first place. The good news There are many excellent mentors ... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post... which studies immunity and the host-pathogen interface, previously developed a high-throughput neutralization...the virus to gain entry into human cells via human ACE2 receptors. In their assay, the pseudoviruses express...reporter which is used as a readout for infection of ACE2-expressing target cells. Any neutralized pseudoviruses... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...tech companies using only laptops and some server space. But what if you want to be an entrepreneur in ... graduate students with innovative ideas in all facets of the entrepreneurial process in order to develop...you start at a disadvantage in the health science space if you don’t have an advanced degree behind your... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a.m. many ...format I decided on was to have two teams of 4 people face each other on a single chosen clinical disease case...they agreed on each element to use, they had to place the selected card on the board game. They had 35... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post... limited by the need for a specific PAM (protospacer adjacent motif) in order to bind the target sites...mediated a C to T conversion in the target sites adjacent to an NG PAM in human cells. The increased versatility... -
K. phaffii: Rising to the Occasion
TypeBlog Post...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression... -
The Importance of High Titer for AAV Transductions
TypeBlog Post... that cell. Since there is a limited amount of surface area on each cell, the way to get more AAV in contact...expression. How do you achieve high titers in the first place? Some AAVs are known to be difficult to produce ...concentration filter column, make sure to rinse off the surface of the filter membrane with the surrounding solution... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...complexity of antigen processing events that take place within an antigen-presenting cell like a T cell.... -
Pushing the Limits of DNA Assembly
TypeBlog Post...where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it becomes... which is freely available online. Its simple interface can guide you through both experimental and primer...