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We narrowed to 970 results for: Abo;

Showing: 261 - 280 of 970 results

  1. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

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    Blog Post
    ...her better! How did you first hear about Addgene?  I heard about Addgene from Joanna Brownstein, an Addgene...Additional resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...money for childhood cancer through the sale of art. About two years ago, I had a big role in organizing an...making, reading, sailing, and gardening. Learn more about Julie Cicalese  Ms. Cicalese served as the Chief...

  2. Science Communication Snapshot: DayCon 2015

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    Blog Post
    ...variety of short- and long-form written content about timely scientific topics, as well as archived seminar...members gave up a beautiful summer Saturday to learn about science, but they didn’t seem to mind! Attendees...consistently mentioned how pleased they were to learn about these exciting scientific topics at a level accessible...other attendees and getting the chance to hear more about what life is like as a student researcher. The future... with the community? Addgene would love to hear about your efforts to engage the public - let us know ...know in the comments section! More information about SITN: DayCon event website (includes archived content...

  3. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ... Learn about research applications of antibodies in Introduction to Antibodies Learn more about fluorescence...Stay tuned for a future blog post to learn more about these techniques!  Direct vs. indirect immunofluorescence...different approaches to be familiar with when talking about IF, direct and indirect. For direct IF, researchers...immunofluorescence procedure with key considerations about each step. Image created with BioRender.com ...of protocol you will need to use. (To learn more about antibody validation, this commentary by Uhlen, et...Fluorescence Microscopy Techniques is Best for Me? Read about another common antibody application in The Basics...

  4. Filming Science Videos in the Age of Social Distancing

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    Blog Post
    ... Production Specialist, I’m constantly thinking about ways to improve our productions to deliver the best...four months after I started with Addgene, I was about to have a check-in with my boss Jennifer. I had ... would be a couple months. Jennifer and I spent about an hour that morning scrambling. Everything we had...someone to be comfortable on camera while thinking about things like lighting, camera angle, and shot composition... allowing people to sit without having to worry about getting in close to mic them up. Monitor: I then...first video in our Intro to the Lab Bench series about PPE! We made this with a combination of in-lab filming... Video production, just like science, is always about iteration and improvement. You have an idea, you...

  5. Stepping into a Virtual Internship: My Experience at Addgene

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    Blog Post
    ...Something that I struggle with as I’ve been thinking about my career options is being an overthinker. Being...Stepping into this new virtual world, I was worried about how my ideas would be translated through Zoom calls...Series on Instagram. I interviewed fellow Addgenies about their role in the company. One of the things I wanted...Specialist. Both of these Addgenies really made me think about my future. They made me rethink having a structured...not only in terms of its employees but in general about anyone who steps foot through their door.  In my...just an intern but I was an Addgenie. I thought about all that could go wrong with this internship but...

  6. Future of Research Conference - Remarkable Opening Session

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    Blog Post
    ...shown in photo), has been speaking out and writing about issues in our science training infrastructure for...some years.  He spoke eloquently and vehemently about the need for change.  Dr. Bourne strongly believes...fact that the PhD and Postdoc advisors only know about academia and alternate training needs to be provided...have to be in academia to make change.  He talked about his own unique way of funding basic research at ...at NEB and got some applause when he talked about how he boycotts publishing in all but open access journals...postdoctoral program.  His strongest comments were about the astounding lack of reproducibility being recognized...

  7. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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    Blog Post
    ...be used in people until we learn more about safety and more about the how to use genome edited stem cells...because Jennifer did her graduate work one floor above mine in the lab of Dr. Jack Szostak, who went on...ceremony honored Charpentier and Doudna, who talked about their research leading up to the discovery of the...Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity” driven project. Both...importance of the strong, cross-disciplinary collaboration they shared to the work they did together. They...

  8. London Calling - Addgene’s New Europe Office in London

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    Blog Post
    ... outreach and community engagement. Information about contacting Addgene can be found on our Contact page...Europe office located in London and operated in collaboration with LGC Standards. And now you’ll be able to...ll be poised and ready to answer your questions about Addgene, plasmids, and depositing (all while our...carrying out my studies, and getting them from collaborators was extremely helpful, but with hindsight I ... and Australia, viewing science as a global collaboration. This is why she is excited to be working at...This love of interacting with people and talking about science makes her the perfect Addgenie and she can...

  9. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post
    ... Delivery Methods Learn about Mouse Work on Exercise and Depression Read About Brainbow Resources on ...success is not always guaranteed. To learn more about this process check out our Mouse Modeling blog posts...by CRISPR. The sequence started clean and then, about 100 bps into the run, it got “dirty”, with multiple...makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease...by Southern blot. From the efficiency discussed above extends 4 major advantages of CRISPR mouse editing...targeting. It also costs less. With CRISPR, it may cost about $5,000 to generate founder mice, while with conventional...

  10. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    Blog Post
    ... post if you debating about an academic or non-academic career Learn more about alternative science careers... written for selfish reasons: I wanted to learn about careers in science communication. When I started...Communication Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest...option. So if you’re interested in learning more about science communication careers, you’re in the right...careers here Resources on Addgene.org Learn more about careers at Addgene ...

  11. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    Blog Post
    ...Additional Resources on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene...the American Cancer Society, or maybe you think about a local food pantry or community arts organization...community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is strength ...accelerate research with easier sharing and collaboration. Joanne and all the Addgenies: Adam, Amanda ...Connie Cepko Resources on Addgene.org Learn More About Our Mission Meet the Addgene Leadership Team Get...

  12. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post
    ...Reddit AMA  Few of us spend much time thinking about conserving resources in the labs. Disposable plastic...Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab in the Ivory ...didn’t feel the need to reuse plasticware or think about conserving reagents and buffers.  However, I had...environmentally conscious friends who had me thinking about energy conservation even before it was a popular...Turns out, one could make a lifetime supply for about $10 US dollars worth of glass powder pruchased from...So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine shop...

  13. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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    Blog Post
    ...mentoring group (see above). However, now that we are all so good with video calls, how about trying your hand...blog or podcast, initiated and maintained a collaborative project, served on the board of a professional...appointments with people all over the world. Think about contacting alumni of your lab or university or use...suggest you speak with. See this post that’s all about informational interviews, for more details on how...progress on both building relationships and thinking about future career options. 5. Write a grant - even if...

  14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post
    ...the Addgene blog Learn more about basic viral vector elements Read about four considerations for the ...Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best... Other cis-regulatory elements Next let’s talk about some of the elements that aren’t required parts ...the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression in retinal cells Resources...

  15. Extracurricular Activities for a Strong Science Career Path

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    Blog Post
    ...This great resource allows the undecided to learn about a variety of science professions based on their ...training focuses on using multidisciplinary, collaborative, and translational approaches for scientific...skills. Although the word “extracurricular” may be taboo in the lab, multiple research studies highlight ...social media (Twitter, Facebook, etc.) to learn about available jobs, fields, and trends “Never Leave...from your lab bench.  Want further information about shaping your career to your values, interests, and...Let us know what topic you'd like to learn more about next. Leave your suggestions in the comments section...

  16. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post
    ...”) of DNA, have changed our collective thinking about the feasible size and scope of projects in many ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...daunting for large numbers of samples. As mentioned above, one of the primary technical benefits of plexWell...1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and the amount...NGS readout also reveals additional information about the presence of non-plasmid DNA or heterogeneous...   Check out the SeqWell website to learn more about our plexWell kits and services! Many thanks to Joe...

  17. Creating accessible biology activities in schools with BioBits

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    Blog Post
    ...student at MIT. While I had always enjoyed learning about biology in high school, it wasn’t until I started...college that I really fell in love with it. Something about being able to actually hold all those seemingly ...seemingly abstract biological reactions that I learned about from textbooks just made everything click in my head...students build circuits in physics class to learn about electronics or mix chemicals together in chemistry...a power source, but now you don’t have to worry about operating the rest of the car as well. Similarly...with us! We’ll work with you to get the word out about your plasmids and their potential educational uses...

  18. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post
    ...Learn About our Cancer Colleciton Pages Use the ClonTracer Library for Lineage Tracing Learn About Cancer... For many years, we’ve hoped that large scale laboratory-based functional experiments that classify which...Collection). In addition, we worked with many collaborators to aggregate a sequence-confirmed series of ..., we used the cancer plasmid collections listed above to demonstrate how gene expression profiling, systematic...with the data generated from these alleles in the above publications), it will be possible for many others...

  19. A Quick Guide to a Career in Software Product Management

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    Blog Post
    ...more about their needs and any problems that they are trying to solve. After learning more about the stakeholders...the company and then go to grad school. However, about a year and a half into my role, I realized that ... my product management (PM) career. Had I known about product management earlier on in my career, I’d ...interests. As such, I want to be able to share more about this nontraditional career path for others who love...management.  A day in the life  Now that you know a bit about what a software product manager does, you might ...

  20. Clear and Accessible Writing

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    Blog Post
    ..., we — Jo, Brook, and Angela — most often think about accessibility in terms of the content we write for...provide visually impaired visitors with information about images that a sighted reader would glean from the...information in the paper.  Of course, when you think about writing an entire scientific paper, you may feel...done? What are we trying to fundamentally change about their knowledge?    Here, this image is being used...extract virus from the correct layer.  Third, think about the venue you are using to present the image and...participants to do the same thing, and even though only about 10% of them had heard of alt text before, they all...
Showing: 261 - 280 of 970 results