We narrowed to 345 results for: Ada

1. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...provided by the major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but...

2. Visualizing Protein Turnover In Situ

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   Blog Post

   ...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins...

3. Plasmids 101: Cre-lox

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   Blog Post

   ...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

4. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...

6. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   ...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   ...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...

8. Hot Plasmids February 2024

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   Blog Post

   ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

9. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   Blog Post

   ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

10. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...

11. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...triggers a cascade of events that results in the degradation of the target mRNA and silences its expression...

12. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post

    ...to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen...

13. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    Blog Post

    ...Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment...

14. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...Project One such space is the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace...

15. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...validating this method through monitoring GFP degradation, this protein compartment was used to optimize...

16. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...

17. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made...

18. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA...

19. Plasmids 101: Protein Expression

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    Blog Post

    ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

20. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post

    ...colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth...