We narrowed to 277 results for: Alk

1. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   ...R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, Musunuru K (2014) Low Incidence of Off-Target...

2. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   ... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann...

3. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   ...declare your bench “RNase free” and refrain from talking to friends and colleagues while working with these...

4. Building Global Connections with the International Mentorship Program USA-EUROPE

   Type

   Blog Post

   ...Boston University, Johns Hopkins University, the Salk Institute, and the Karolinska Institutet, among ...

5. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   ...choice? And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks...

6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

   Type

   Blog Post

   ...that are essential for experiments but that nobody talks about. After months of troubleshooting, those people...

7. Custom CRISPR Screens & the Green Listed Software

   Type

   Blog Post

   ... interest. Selected pathways  I attended a great talk when I was a PhD student. In particular, one slide...

8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

   Type

   Blog Post

   ...dyes with different emission spectra to avoid crosstalk/crossover to reduce ‘muddying’ of the signal and...

9. Special Delivery: Fluorophore Targeting for FRET Studies

   Type

   Blog Post

   ...s best to consider these cases separately when talking about the tools that are available. Let’s consider...

10. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    ...the intended brain region and/or cell type (I’ll talk more about this in detail later). But what are the...

11. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    ... and yes those purification columns we’ve been talking about!) are a significant source of waste in laboratories...

12. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    .... K., Roberts, N., Zhang, L., Vakulskas, C. A., Walker, J. A., Kadina, A. P., Zepeda, A. E., Holden, K...

13. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    ... if you’re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is ...

14. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    ...nucleoli, and little cytoplasm. Positive staining for alkaline phosphatase, expression of embryonic proteins,...

15. Deep Dive: qPCR

    Type

    Blog Post

    ...doi.org/10.1093/bioinformatics/btr065Freeman WM; Walker SJ; Vrana KE. 1999. Quantitative RT-PCR: pitfalls...

16. 15 Hot Plasmids from 2017

    Type

    Blog Post

    ...robust initiation of reprogramming (evaluated by an alkaline phosphatase-positive assay) as well as stabilization...

17. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    ..., you’re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting...