...and incubation times. If the vendor includes publications that cited the antibody, consider reviewing ... the materials and methods sections of those publications.  Finally, always verify that the chosen antibody...

...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...nevertheless still lead to activity has been established, and can be used to identify sites that are likely...

...were Spanish scientists (MD, PhD and MD/PhD) established in some of the top Universities in USA and Europe...from different Spanish universities and high level public and private institutions also showed their support...

...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...Mouse line with conditional deletion of Xist Unpublished Joost Gribnau Human Cell Line Models Induced ...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link....806delG 806delG G269Afs*20 M Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold A140V...A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy Gold AN_BU 808delC Arg270Glufs...Arg270Glufs*19 F Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold BO_DI 806delG G269Afs...G269Afs*20 F Fibroblasts Unpublished (Link opens in a new window) Wendy Gold HO_AN G917A R306H F Fibroblasts...

... As scientists, we probe beyond the realm of established theory. Realizing that textbooks don’t tell us...research in basic biology, medicine, epidemiology, public health, and more.     A few of the most common...

...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection...

...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite...model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39. PMID: 26310623... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite....9 When using the AAV44.9 serotype in future publications, please acknowledge John Chiorini and Shannon... using the AAV44.9(E531D) serotype in future publications, please acknowledge Shannon Boye and cite Boye...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...

...reagents, we scaled up these tagging experiments by assembling csRNPs in a 96-well plate format and tested parallel...Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. ...

... has published information and protocols for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus...Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing DETECTR... reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV...Qi's lab. COVID-19 Diagnostic Toolkit Enzymes. (unpublished) Taq polymerase and MMLV reverse transcriptase...Starr). External Resources Research Articles Many publishers have allowed free access to articles related ...of COVID-19 related articles spanning multiple publishers can be found here: ASM COVID-19 Research Registry...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ...

... ID Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...editing variants Four years after their initial publication, the Liu lab returned to the reverse transcriptase...

...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate...

...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID ...Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...note that the following table lists both published and unpublished plasmids containing gRNA sequences designed...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...CRISPR nuclease and function. Please see the publication or plasmid information page, or contact the depositing...plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...control). The end result of the meeting will be a publicly shared practical guide for all scientists in the...prepare and compile resources for this meeting's publication, we are asking participants to share practical...have final approval before anything is released publicly, but please only share materials that you are ...materials that you have permission to share (e.g., publication's permission). Connie Cepko, Harvard Medical School...section for how to best help contribute to this publication. Citation information for openly shared communication...

...CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter Overview...Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors...can be made. Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into... before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology...early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue of Nature...