We narrowed to 382 results for: Bre;

1. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   ... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched...

2. Targeted Mutagenesis with EvolvR

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   Blog Post

   ...tracing cells that do not tolerate double-strand DNA breaks by introducing a unique tag into a genomic site...

3. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   Blog Post

   ... it available to everyone, that creates more breakthrough and innovation. With any kind of open platform...

4. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ... nuclease, which introduces double stranded DNA breaks, can be expressed from lentiCas9-Blast. Purpose...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...offers high efficiency of inducing double strand breaks and more opportunity for homologous recombination...

6. Plasmids 101: Blue-white Screening

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   Blog Post

   ... When expressed, the β-galactosidase enzyme can break down a dye-linked substrate called x-gal (5-bromo...

7. How to Make Friends and Meet People at a Scientific Conference

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   Blog Post

   ... you are interested in and this is a great ice breaker. Of course, real relationships go further and grow...

8. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...the day cannot be developed through conventional breeding; synthetic biology is required to achieve this...

9. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   Blog Post

   ...(A) Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible. ...

10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ...schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...

11. dTAG - You're it!

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    Blog Post

    ...not necessary for the proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693....

12. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...AAV8 vectors with broadly active promoters from subretinal injections of neonatal mouse eyes at two different...

13. Plasmids 101: The protein expression toolbox

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    Blog Post

    ...transcription instead of introducing a DNA double strand break. Transient introduction of CRISPRi or inducible ...

14. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...obvious explanation, but more often than not I had to break down exactly what I had done in order to identify...

15. What's New in CRISPR - November 2021

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    Blog Post

    ...transcriptase (RT), which induces two double-strand breaks and deletes the intervening sequence. As with other...

16. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ...study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors...

17. RNA Extraction Without A Kit

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    Blog Post

    ...broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late 1970’s, Chirgwin...

18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ... different stages of development, ensuring the breadth and versatility of this tool. Find CreLite at Addgene...

19. Visualizing Protein Turnover In Situ

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    Blog Post

    ... tags influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific...

20. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...function of guiding Cas9 to generate a targeted break. These advances in gRNA technology have enhanced...