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We narrowed to 641 results for: CAR

Showing: 261 - 280 of 641 results

  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... it, virtually every milestone in a scientist's career is mediated by some kind of communication to another...communication will be a positive asset throughout your career? 1. Make scicomm practice a priority The good news...Additional resources on the Addgene Blog Learn about careers in science communication The strength of story ...

  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...Cas9-expressing lentiviruses will no longer be carried over to the gRNA lentiviruses. This means that ...from Bryan Roth’s lab at the University of North Carolina, Chapel Hill. These can be used to investigate...pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry...

  3. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...can then build on this research and effectively carry out their mission of creating new forums and opportunities...were generated by large agricultural companies and carry associated intellectual property concerns – patents...to construct our color changing flower we are carefully making our way through the IP landscape, using...

  4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...that my research will have a direct impact on healthcare and the community. As a result, I have occasionally...target and light-up bacteria unwanted in food, healthcare settings, etc. What project in the Collins ...co-founder of a biotech startup, Mike explained, his career transitioned away from the bench and became all...

  5. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...makes them that way. Is there a senior person whose career path you are interested in following? Someone you...notify your network that you’re on the hunt for career advice. Your family and friends know you well and...groups will often have mentoring programs. The Healthcare Businesswomen’s Association hosts an excellent...

  6. Antibodies 101: Conjugation

    Type
    Blog Post
    ...While the antibodies present throughout our bodies carry out plenty of roles just the way they are, the research...lysines, sulfhydryl groups from cysteines, or carbohydrates from glycosylation (Dennler, et al., 2015). ...bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally. 4)...

  7. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...anyone who is planning or might need to plan for a career outside academia (that would be pretty much all...managers will think it is odd. For those planning a career in academia, it may not be required, but many academic...You can use LinkedIn before an interview, but be careful If you are lucky enough to have a schedule before...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    ...confidential process among a few individuals.  Early-career researchers especially enjoy the ability to post...Puebla I (2022) A guide to preprinting for early-career researchers. Biol Open. https://doi.org/10.1242...Hensel Z (2019) On the value of preprints: An early career researcher perspective. PLoS Biol. https://doi....

  9. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...recombination scar encoding eight amino acids, but Golden Gate assembly can be designed to be scarless. Golden... in the final construct, allowing for precise, scarless cloning. The cloning scheme is as follows: the...

  10. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Conveniently, plasmids carrying genes for bacterial toxins like colicin typically also carry the necessary resistance...century! Nevertheless, the transfer of plasmids carrying these genes between bacteria can also have serious...

  11. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...ultimate goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020...JC, Shon DJ, Fox DM, Puschnik AS, Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (...Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy KR, Lavine CL, Zuo T, Lin J, Zuiani A, Feldman...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...which means it requires multiple Cas effectors to carry out genomic editing. Specifically, these types of... using a gRNA and can then recruit Cas3, which carries out the brunt of the mechanical work for editing...The reeling mechanism The reeling mechanism is carried out much like the name implies — after nicking,...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... Andrew and Maria were skeptical. The team was careful to test the possibility that this might just be...proteins that had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random...semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of improved...

  14. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...for viral genome sequencing (VGS). Why should you care about viral quality control? AAV vectors are produced... sequence. The resulting list of hits is then carefully reviewed. As mentioned earlier, it is common to...the contamination can be identified, and we will discard any AAV prep we suspect is contaminated. For AAV...

  15. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...advantage over Taqman is price point, which should be carefully balanced against the limitations of the assay....left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains a primer and probe set for...Cloning Techniques References Bell AD, Usher CL, McCarroll SA. Analyzing Copy Number Variation with Droplet...

  16. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...figure at right for an example). Look out for carbohydrates Certain strains, including HB101 and derivatives...derivatives, release large amounts of carbohydrates that can inhibit the cell lysis process. To ensure that..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation...

  17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...Transformed cell lines Transformed cell lines Cargo Nucleic acids or protein Nucleic acids Nucleic acids...Microinjection Cells Many types Single cells Cargo Nucleic acids or protein Nucleic acids or protein... nondividing Dividing Dividing or nondividing Cargo Nucleic acids Nucleic acids Nucleic acids Throughput...

  18. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...spread even when costly. If even a single organism carrying a synthetic gene drive were to escape the laboratory...trust in scientists to develop new technologies with care and humility. Nothing would damage that trust more...aaa5945 (2015). doi:10.1126/science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M....

  20. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...groups of trainees at an earlier point in their careers? A CURE for Biochemistry Undergraduate biochemistry... then proceed to use those skills to design and carry out their own novel experiments in semester two ...as they increase student interest in scientific career paths while also broadening access to experiences...
Showing: 261 - 280 of 641 results