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We narrowed to 572 results for: Cit

Showing: 261 - 280 of 572 results

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...AAV.PHP.eb for neuron, oligodendrocyte, and astrocyte specificity and would like to get it working in microglia...development of more and smaller CRISPR tools is an exciting area for future AAV application to epigenetic ...

  6. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...perform retrograde tracing, but often at the cost of toxicity, low expression, or poor scalability.  Scientists...neurons within their natural environment, all while reciting their favorite slogan, “look how cool the brain...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So let’s break ...engineered receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics...

  8. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...internships share a common set of criteria such as citizenship status, academic year, and grades that serve ...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ...

  10. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...starting our viral vector service in 2016...we’re excited to help scientists share their reagents with the... for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years ...

  11. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...for our newsletter and a free plasmid) were very excited about our new viral services. If you’re not familiar...educational content on the blog. Overall, there are many exciting things going on in the world of neuroscience and...

  12. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...active throughout the cell cycle and has a higher capacity for repair, as there is no requirement for a repair...RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016...

  14. Addgene: The First Twenty Years

    Type
    Blog Post
    ...big plans for our future. And while we’ve been excited to share them with you, we realized our 20th anniversary... millionth item, and had a cumulative 120,000 citations of individual items in our repository, a tribute...

  15. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...protein to create three distinct colors. The specificity of these orthologs is key: since each ortholog...

  16. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...can fail but, in our current setup, there are no explicit instructions on what to do when something fails... We can set ourselves up for success by giving explicit instructions to lab members through the software...

  18. Hot Plasmids February 2024

    Type
    Blog Post
    ...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

  19. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

  20. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed in post...variant you wish to use to determine the ideal excitation). Lasers can be purchased through Laserglow, ...
Showing: 261 - 280 of 572 results