...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression in striatal ..._3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression in striatal ...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression in striatal ...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...

...Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through injection...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems and Delivery Methods Learn about Mouse...specifies the target, the Cas9 endonuclease that creates the DNA double-strand break (DSB) at the target...knock in models). CRISPR mouse model basics To create a mouse model, the gRNA, Cas9, and donor oligonucleotide...selection offered by mouse ESCs. For example, we often screen only 15-25 mice when generating knockout models...for generating mouse models Although CRISPR is incredibly useful for generating mutations by NHEJ and generating...

...drawback of CRISPR-Cas9 technology is that most expression systems are constantly “on”. There is no mechanism....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...

...viral vectors (here and here).  Figure 3: JEDI-2P was identified from a high-throughput screen, selected...Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR.... By fusing ITAM to an RTK of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence... They use a unique and powerful high-throughput screen to select variants based on performance metrics...resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity, and photostability under...selected for increased voltage response, brightness, and photostability. It enables sustained deep-tissue... be generalized to other RTKs of interest, they create sensors for FGFR1, EGFR and ErbB2. And orthogonal...

...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are described.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...which an exogenous mRNA can be injected and expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that ...A useful “starter kit” for screening fluorescent protein fusion expression in organisms or cells in which...toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...

... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These....1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct fluorescence...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...

...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses... 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also see pMIG-w , a variant that...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...pLncEXP CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...

... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none S. pyogenes Huang...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...

...Edit Collection The CRISPR Guide Empty gRNA Expression Vectors... we performed high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled ...cell contexts. Figure 1: High-throughput screening of pegRNA efficiency and building prediction tools...sequence-based prediction models from self-targeting screens such as PRIDICT/PRIDICT2.0 provide valuable insights...your target. Figure 3: High-throughput screening of the effect of chromatin on prime editing efficiency...

...along with Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal...on the Addgene Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...

...cells expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in...Fon EYFP Cre AND Flp 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp 55651 pAAV-hSyn Con/Foff EYFP Cre AND NOT...Flp AND NOT Cre 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT Flp...AND NOT Cre 137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry Cre AND NOT...AND NOT Cre 137122 pAAV-Ef1a-Con/Fon-GCaMP6F Cre AND Flp 137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F Cre AND NOT...Fon/Von-EYFP Cre AND Flp AND Vcre 137165 pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre References ...

... vitro, and can be used for measuring protein expression or localization in cells, or even single-particle...Mol Syst Biol. 2014 Galit Lahav EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog of the...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj...celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured in our blog ...Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and Mengtong "Tom" DuanVisualizing...Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene writers High Complexity...

... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases...Deisseroth 55636 pAAV-EF1a-Cre EF1a none rg* Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1,...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion...105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Carmichael...Carmichael 107788 AAV.rTH.PI.Cre.SV40 rTH none 9, rg* Wilson 24593 AAV-pgk-Cre PGK none rg* Aebischer 51507...51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8...

...His-tagged bacterial expression vector (see tagged expression vectors from the SGC... Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi...destination vector for gene expression URA3 Yeast pXP518 - Yeast expression ... Gateway expression vector (see more Gateway vectors) TRP1 Yeast pXP216 - Yeast...Yeast expression vector (PGK1 promoter, 2μ) pXP316 - Yeast expression vector... Gateway expression vector (see more Gateway vectors) LEU2 Yeast pXP122 - ...- Yeast expression vector (PGK1 promoter, CEN/ARS) pXP222 - Yeast expression ...

... empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...and spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from...can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily...developed and characterized which allow researchers to express desired constructs in precise cell types and/or...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...

...manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank for feeding...continues to be challenging. Organism specific viral vector systems would also be beneficial for many systems... progress on the NSF “G to P grand challenge.” Creating a productive, new scientific community is no small...in particular, that they all share the plasmids created under this programs via Addgene. New organisms ... organisms) -  perhaps you’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project...crisis. I like how they are using protocols.io to create a protocol community! Asclepias syriaca and Asclepias...used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black footed slipper...

...These proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate...identified to increase the spectral and kinetic properties as well as improve the expression and membrane...fluorescent protein to the opsin in order to track its expression and localization. Many other types of proteins...inhibition. For example, search for "ChR mCherry", "Cre inhibition", "Boyden 2014", or other terms. Looking...optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication Return to top Optical...other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication Return to top Do you...

...with natural chain termination at stop codons, expression levels of these tagged proteins are often very...could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA ...intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...donors (Fig. 1). Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence...

...Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Calcium Intersectional viral expression of sRGECO (constitutive or Cre- or Flp-dependent) Comprehensive...Adenosine Express the adenosine sensor GRAB_Ado1.0 in neurons or astrocytes, (Constitutive or Cre-dependent...cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr: A single-wavelength fluorescent...iSeroSnFR for bacterial or mammalian expression (Constitutive or Cre-dependent) Directed Evolution of a ...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...