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We narrowed to 369 results for: Cre expression vector

Showing: 261 - 280 of 369 results

  1. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... groups was to create a system that would use light to turn on and off gene expression while imparting...HEK293T cells to induce expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after...Phillips-Cremins JE (2019) LADL: light-activated dynamic looping for endogenous gene expression control...transcriptional activator domain (VP64 or p65). Upon expression in the cell, the dCas9-CIB1 fusion binds to the...exposure (470nm), the researchers showed that expression of the human ASCL1 gene could be spatially controlled...demonstrated the ability to spatially control gene expression. Both of these techniques take advantage of light-induced...light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal fragment of Cas9 while transcription...

  2. AAV Molecular Tools

    Type
    Collection
    ...amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent...FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP...2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby...Name Expression System Activity Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent...pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and...pAAV-FLEx-ER-TurboID CMV-driven, Cre-dependent Cre-dependent expression of ER-localized TurboID (with HA...pAAV-FLEX-P301L Tau EF1a-driven, Cre-dependent Cre-dependent expression of human 2N4R microtubule-associated...

  3. Retrograde AAV viral preps

    Type
    Collection
    ...AAV-pgk-Cre PGK Cre expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate...GFP-tagged Cre expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases... 0.4.Cre.SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells...physically-separate mCherry expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases ...simultaneous dTomato expression Recombinases Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases...pAAV-EF1a-Flpo EF1a Flpo expression Recombinases Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases...

  4. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...changes in root-to-shoot ratio in Arabidopsis, (2) expression patterns in plants with enhanced sodium accumulation...protocols Find eBooks on plasmids, CRISPR, viral vectors, and more ...the community, increasing the impact of the work, and as a collateral benefit, increasing the impact of...Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, and Nature, 1945–1990." Notes and...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase. Takuji...63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase. Floris...Neo-TRE-CMV-Cre-rtTA Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression You may also like... Blog...

  6. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of...purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure... (although we could successfully knock-in non-expressed genes with similar efficiency, Dewari et al. 2018...tagging success, although many of these genes were expressed at really low levels so actual knock-in success...antibody” can be used for downstream IP/ChIP assays. Creating epitope-tagged clonal cell lines remains a challenge...crRNA complementary to the template strand could increase knock-in efficiency.Note: Our TAG-IN tool can ...

  7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...targeted repression of gene expression. They successfully knock down expression of essential and non essential...) technology (Ronda et al., 2016). pMA7CR_2.0 expresses lambda Red and Cas9, which are separately inducible...function in bacteria, most efforts to regulate gene expression were limited to inducible promoters or direct... much more user-friendly way to modulate gene expression. The Marraffini lab and Qi lab developed early...repression. Using a Tet responsive promoter to drive expression of a catalytically dead Cas9 enzyme allows reversible...the gRNA cassette, one can reliably knockdown expression of multiple target transcripts simultaneously...genes in M. tuberculosis. dCas9 and a gRNA are expressed on pRH2502 and pRH2521, respectively. Both dCas9...

  8. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...search scoped to plasmids shows filters for expression and vector type, while a search scoped to viral preps...well-characterized. During this time, we’ve also created a variety of educational resources in an effort...understanding of Addgene’s customer’s needs. Categories We created a categorization of our information in order to...for promoter and serotype, among others. We also created a normalization mechanism to “collapse” variations...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...Cre-expression Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector Packaging Service... a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These viral vector preparations were produced...Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent ...sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent...sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent...

  10. Brain Armamentarium

    Type
    Collection
    ...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression in striatal ..._3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression in striatal ...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression in striatal ...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...

  11. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through injection...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems and Delivery Methods Learn about Mouse...specifies the target, the Cas9 endonuclease that creates the DNA double-strand break (DSB) at the target...knock in models). CRISPR mouse model basics To create a mouse model, the gRNA, Cas9, and donor oligonucleotide...selection offered by mouse ESCs. For example, we often screen only 15-25 mice when generating knockout models...for generating mouse models Although CRISPR is incredibly useful for generating mutations by NHEJ and generating...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...Empty gRNA Expression Vectors page Cre-lox Site-specific recombination See our dedicated Cre-lox Plasmids...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-...versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins ...Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are described.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...which an exogenous mRNA can be injected and expressed. EXPRESs Plasmids for Zebrafish Receptors and Zebrafish...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that ...A useful “starter kit” for screening fluorescent protein fusion expression in organisms or cells in which...toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...

  14. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...drawback of CRISPR-Cas9 technology is that most expression systems are constantly “on”. There is no mechanism....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...

  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...viral vectors (here and here).  Figure 3: JEDI-2P was identified from a high-throughput screen, selected...Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR.... By fusing ITAM to an RTK of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence... They use a unique and powerful high-throughput screen to select variants based on performance metrics...resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity, and photostability under...selected for increased voltage response, brightness, and photostability. It enables sustained deep-tissue... be generalized to other RTKs of interest, they create sensors for FGFR1, EGFR and ErbB2. And orthogonal...

  16. Viral Production

    Type
    Collection
    ... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These....1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct fluorescence...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...

  17. Retrovirus Plasmids

    Type
    Collection
    ...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses... 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also see pMIG-w , a variant that...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...pLncEXP CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...

  18. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none S. pyogenes Huang...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...

  19. Mammalian RNAi Tools

    Type
    Collection
    ...constitutive expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...interference. Created with BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing...Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ...cells expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in...EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff EYFP Cre AND...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT.../Von-EYFP Cre AND Flp AND Vcre Yes 137165 pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre No References...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable...
Showing: 261 - 280 of 369 results