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We narrowed to 824 results for: EED

Showing: 261 - 280 of 824 results

  1. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...select a candidate cell line (e.g. DepMap) – you’ll need to ensure that the parental cell lines of your chosen...will depend on the protein turnover rate. If you need a more permanent knockdown, consider using shRNA...of RNA knockdown by RT-qPCR, and potentially the need to study protein expression at multiple timepoints...challenge to confirm. As such, the approach used may need to vary according to the target, sample type, and...cell types are available. Further evidence is then needed to confirm that the staining represents the target...

  2. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...P3 laboratory (BSL-3), a high-security facility needed to perform the experiments to demonstrate that ...every day.    Figure 2: When a researcher needs a collaborator for a specific task, Crowdfight will...information to deliver to the requesters what they needed. In the example above, for example, we fostered...encourage you to file a request on our website if you need help with your scientific research. It’s completely...

  3. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have...thank you note or gift to someone who helped you succeed in any way. Don’t believe people who tell you that...mutual acquaintance connect you to someone who you need to speak to for informational or career purposes...experience for you both, they will be there when you need them. I guess the bottom line is meet people, don...

  4. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... stronger and less dependent on the manager to succeed. More people in leadership roles leads to more...you are not delegating enough. For example, if we need to develop a new plasmid collection page, we certainly...manager must do. You can’t delegate the giving of feedback or performance review. You can’t delegate tasks... Can you provide the information and resources needed for the assignment? Setting someone up for failure...

  5. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...perhaps challenges) is the need to present yourself using a resume. Indeed, instead of having all the ...sometimes CVs are. Because you only have two pages, you need to have a good idea of what should be highlighted... the skills you already have, and the skills you need and don't have yet for that dream job. Also, many...

  6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...fluorescent proteins to be imaged together, you will need to consider the same factors as when choosing an...discussion of these factors). In addition, you will also need to choose fluorescent proteins that can be distinguished... require biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal...and that if you want the brightest signal, you may need to try several proteins in your system of interest...

  7. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...plasmids and their ability to identify and fulfill a need in terms of helping the scientific community manage... platform, there is the potential for increased speed and efficiency through which knowledge is created...programmatic content as well as help our students succeed whether that be academically or on the employment...great things in a particular area or profession, you need to make sure you have learned and mastered the fundamentals...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...plasmid service was started based on donations. That seed money was used to build a completely self-supporting...similar model for our Viral Service, we solicited seed money for the new project. New England Biolabs and...ready-to-use viral format. Having identified this need in the community, we approached it in the way Addgene...interesting things going on at Addgene. Sharing speeds science and we are proud to be adding viral vector...

  9. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your...basics of controls in experimental design, you’ll need a few controls specific to flow as an application...looking at and the antibodies you are using, you may need to include additional controls like FMO or isotype... is some debate in the literature as to when you need isotype controls. Our lab prefers to use them to...

  10. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...’ve just made it easier for you to find what you need quickly.  Through the years, we have perfected ...search function. We also quickly realized that we needed to improve more than that: the general “findability... have different strategies for finding what they need, and we wanted to support all of them — searching... our deep understanding of Addgene’s customer’s needs. Categories We created a categorization of our information...

  11. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...study gene function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs...phenotypes than standard RNAi protocols without the need for mutant genetic backgrounds. The standard RNAi...creating a pair of enzymes that overcome both the speed and toxicity limitations of APEX2 and BioID. In ...

  12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... and the things you need to include in your manuscript. What information do I need to include in my paper... to anyone who wishes to read it, without them needing a subscription to the journal. An increasing number...reviewers and the editor decide that your manuscript needs to be revised before it can be published, this can...

  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...transgene-free and vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce...genomic integration Multiple transfections required Need to sequence to verify there’s no genomic integration...-free cells No genomic integration Inefficient Need to verify loss of episome Minicircle Non-integrating...-free cells No genomic integration Inefficient Need to verify loss of minicircle RNA Delivery DNA-Free... difficult to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...Addgene's resident blog editor, I could take the needed steps to continue where the guide left off - namely...conjugates. And, of course, once you have them, you’ll need to either re-develop or re-optimize your assay for...identified but a smaller or less immunogenic version is needed.  Single-chain fragment variables (scFvs) are ~...will arise in your research or what data you may need to collect. Understanding available alternatives...

  15. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...2013). We found that the fluorescent proteins can, indeed, interfere with the correct function of the protein...this was problematic, because the DREADD receptors need to be located on the plasma membrane to be activated...DREADDs. In studies where the fluorescence is not needed, the use of HA and other short epitope tags (Kimple...receptors potently activated by an inert ligand. Proceedings of the National Academy of Sciences 104:5163–...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...projects. Right now we don’t have to worry about succeeding at our core mission. Plasmid distribution is ...this and recognize it all the time. We love this feedback and are always adding to our “google doc of gratitude...awhile I worry a very little bit about hiring when we need to do it quickly. Hiring is hard but as a 3 year..., excellent intentions, or incredible drive to succeed.  Thanks to all the Addgenies and congratulations...

  18. Antibodies 101: Conjugation

    Type
    Blog Post
    ...the research antibodies in your refrigerator often need a little help to be useful. Mainly because, well...available, that never guarantees the antibody you need will be conjugated, or that it will be conjugated...conjugation, it is important to keep in mind that you will need to confirm your antibodies’ performance after the...which can interfere with conjugation reactions. If needed, you can often remove the incompatible agents by...

  19. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...stakeholders. However, while most institutions are indeed going ahead with their planned raises, some are...ahead with the raises as they see fit, following feedback from postdocs and faculty (at time of writing,...detrimental effect on the morale of postdocs, and indeed we have been contacted by a number of postdocs ...keep raising salaries and that we are submitting Freedom of Information Act (FOIA) requests for their postdoctoral...

  20. To Each HIS Own

    Type
    Blog Post
    ...how many do I need? When designing a plasmid containing a polyhistidine tag, you’ll need to decide whether... binding without eluting the target protein and needs to be empirically determined. In addition, some ...Longer histidine tags are also recommended if you need a very pure sample. Because binding strength is ...
Showing: 261 - 280 of 824 results