We narrowed to 821 results for: EED

...tag-specific antibody (Jarvik and Telmer 1998). Indeed, this method can be used at high throughput because... each cell type, the transfection/delivery step needs to be optimized to achieve high knock-in efficiency...molecules can be directly purchased and there is no need to construct plasmid vectors or synthesize/purify...lab is new to CRISPR/Cas9 experiments, there is no need to worry at all. Setting up tag knock-in experiments...most crucial aspect of knock-in experiments. You’ll need to optimize csRNP delivery for each cell type you...

...literature search to see what others have done, you’ll need to experimentally test your chosen capsid before...a few additional, specific considerations you’ll need to be aware of before starting an experiment. Controlling...are only starting recommendations; you will still need to experimentally determine the best titer for your... If you think you’ve found a good fit (or if you need more options) we recommend you check out Caltech.... K., Monet, D., Ngo, V., Ding, X., Omstead, V., Weed, N., … Gradinaru, V. (2023). Functional gene delivery...C. L., Balazs, A. B., Daneman, R., Bloom, J. M., Seed, C., & Deverman, B. E. (2019). Delivering genes ... A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V....

...Plasmids of course! You just need three plasmids to start making the AAVs you need for your experiment:  the...adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid...regions of dsDNA. To generate a 3’ hydroxyl group needed for DNA replication, Rep78/68 binds the Rep binding...

...discarded.  Seeding Labs does an amazing job shuttling unwanted lab supplies to places that really need them....resourced areas of the world.  Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student...s, my lab was well funded and we didn’t feel the need to reuse plasticware or think about conserving reagents...

...when you wantYou can just turn them offNo need for any washNo need for any wash…” 🎶- Barbara Di VenturaIf... AraC, it still reached the level of expression needed for most applications.   Figure 1: With ...promoter, can be controlled with light without the need of any cloning.     What advantages does BLADE...

...select a candidate cell line (e.g. DepMap) – you’ll need to ensure that the parental cell lines of your chosen...will depend on the protein turnover rate. If you need a more permanent knockdown, consider using shRNA...of RNA knockdown by RT-qPCR, and potentially the need to study protein expression at multiple timepoints...challenge to confirm. As such, the approach used may need to vary according to the target, sample type, and...cell types are available. Further evidence is then needed to confirm that the staining represents the target...

...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have...thank you note or gift to someone who helped you succeed in any way. Don’t believe people who tell you that...mutual acquaintance connect you to someone who you need to speak to for informational or career purposes...experience for you both, they will be there when you need them. I guess the bottom line is meet people, don...

...P3 laboratory (BSL-3), a high-security facility needed to perform the experiments to demonstrate that ...every day.    Figure 2: When a researcher needs a collaborator for a specific task, Crowdfight will...information to deliver to the requesters what they needed. In the example above, for example, we fostered...encourage you to file a request on our website if you need help with your scientific research. It’s completely...

... stronger and less dependent on the manager to succeed. More people in leadership roles leads to more...you are not delegating enough. For example, if we need to develop a new plasmid collection page, we certainly...manager must do. You can’t delegate the giving of feedback or performance review. You can’t delegate tasks... Can you provide the information and resources needed for the assignment? Setting someone up for failure...

...perhaps challenges) is the need to present yourself using a resume. Indeed, instead of having all the ...sometimes CVs are. Because you only have two pages, you need to have a good idea of what should be highlighted... the skills you already have, and the skills you need and don't have yet for that dream job. Also, many...

...fluorescent proteins to be imaged together, you will need to consider the same factors as when choosing an...discussion of these factors). In addition, you will also need to choose fluorescent proteins that can be distinguished... require biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal...and that if you want the brightest signal, you may need to try several proteins in your system of interest...

...plasmid service was started based on donations. That seed money was used to build a completely self-supporting...similar model for our Viral Service, we solicited seed money for the new project. New England Biolabs and...ready-to-use viral format. Having identified this need in the community, we approached it in the way Addgene...interesting things going on at Addgene. Sharing speeds science and we are proud to be adding viral vector...

...’ve just made it easier for you to find what you need quickly.  Through the years, we have perfected ...search function. We also quickly realized that we needed to improve more than that: the general “findability... have different strategies for finding what they need, and we wanted to support all of them — searching... our deep understanding of Addgene’s customer’s needs. Categories We created a categorization of our information...

...plasmids and their ability to identify and fulfill a need in terms of helping the scientific community manage... platform, there is the potential for increased speed and efficiency through which knowledge is created...programmatic content as well as help our students succeed whether that be academically or on the employment...great things in a particular area or profession, you need to make sure you have learned and mastered the fundamentals...

...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your...basics of controls in experimental design, you’ll need a few controls specific to flow as an application...looking at and the antibodies you are using, you may need to include additional controls like FMO or isotype... is some debate in the literature as to when you need isotype controls. Our lab prefers to use them to...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...study gene function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs...phenotypes than standard RNAi protocols without the need for mutant genetic backgrounds. The standard RNAi...creating a pair of enzymes that overcome both the speed and toxicity limitations of APEX2 and BioID. In ...

... and the things you need to include in your manuscript. What information do I need to include in my paper... to anyone who wishes to read it, without them needing a subscription to the journal. An increasing number...reviewers and the editor decide that your manuscript needs to be revised before it can be published, this can...

...transgene-free and vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce...genomic integration Multiple transfections required Need to sequence to verify there’s no genomic integration...-free cells No genomic integration Inefficient Need to verify loss of episome Minicircle Non-integrating...-free cells No genomic integration Inefficient Need to verify loss of minicircle RNA Delivery DNA-Free... difficult to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs...

...Addgene's resident blog editor, I could take the needed steps to continue where the guide left off - namely...conjugates. And, of course, once you have them, you’ll need to either re-develop or re-optimize your assay for...identified but a smaller or less immunogenic version is needed.  Single-chain fragment variables (scFvs) are ~...will arise in your research or what data you may need to collect. Understanding available alternatives...

...how many do I need? When designing a plasmid containing a polyhistidine tag, you’ll need to decide whether... binding without eluting the target protein and needs to be empirically determined. In addition, some ...Longer histidine tags are also recommended if you need a very pure sample. Because binding strength is ...