...your elevator pitch where the other person seemed confused, bored, or particularly excited about? If you ...

...products into E. coli. Ligases within the E. coli then fuse together the PCR products via overhangs left over...

...to the gene of interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy...

... fly stocks and other reagents. If you’re ever confused about the best way to study YFG in flies, there...

... initial model of this protein – but were very confused because the HNH nuclease active site wasn’t even...

...different applications such as fluorescent protein fusion studies and the generation of mutant cell lines...

...system indicates that it is very tricky to use and fussy to optimize. One reason could be the instability...

...construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific...

... single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these...

... bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin...

...inquiry – purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and...

...sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining...

... Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...

...directed binding site. These methods include the fusion of an activation domain such as VP16 to dCas9 or...

...Cas9 expression is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic...

... dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information...peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure...across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a given...

...mStrawberry-N1 - Mammalian Expression FusionRed 580 608 18 Monomer FusionRed-pBAD - Bacterial Expression mRFP1...with different fluorescent tags for you to create fusion proteins with your gene of interest. Special... Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer not a Monomer...with different fluorescent tags for you to create fusion proteins with your gene of interest. These are ...Snapp . Empty Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...to Top Empty Backbones for Fluorescent Protein Fusions (Organized by Expression Species) Organism/Expression...

... containerization, but haven’t tried it or are confused by the concept, don’t worry–you are not alone!...

...envelope protein causes cells that it infects to fuse together and form syncytias that cause the cells...

...also small enough to deliver with AAV. This system fuses catalytically dead dCas13b to the catalytic domain...