We narrowed to 327 results for: INO

1. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   Blog Post

   ...secondary peak around 280 nm thanks to aromatic amino acids like tryptophan and tyrosine (Figure 1B). ...

2. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ... Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino for their helpful discussion of this book....

3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...https://doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to...

4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

5. Antibodies 101: Producing Recombinant Antibodies

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   ... though this can vary depending on the specific amino acid sequence (Maity et al., 2015). If your antibody...

6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ... (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be incorporated into a protein structure...

7. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...histone proteins K4 and K27 – locations of the amino acid lysine in H3 me1, me3, and ac – denoting ...

8. Tips for Screening with Yeast Two Hybrid Systems

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   ...El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, C., Ellerbrok, H., Kamuzinzi, ...

9. Cpf1: A New Tool for CRISPR Genome Editing

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   ...two nucleases, AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered...

10. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ... sample that is transformed that week is then inoculated for storage on Thursday. The lab prepares bacterial...

11. Your Lentiviral Plasmid FAQs Answered

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    ...maximal biosafety available for the technology. The minor limitation in using the 3rd-generation system is...

12. QC Sequencing Technologies at Addgene

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    ...theoretical sequences submitted to Addgene have minor differences due to in silico variations and lack...

13. Hot Plasmids: Fall 2024

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    ...variation on their previous sensor STReTCh, the 11-amino acid BiT fragment of luciferase is embedded in the...

14. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...small DNA binding motif in MECP2 interacts with the minor groove of AT-rich DNA sequences to regulate transcription...

15. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...the BFP mutant (BFPH66) contains a histidine at amino acid 66. This histidine is encoded by a ‘CAC’ codon...

16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...introduce a single nucleotide modification at a defined amino acid to mimic a patient mutation or maybe to disrupt...

17. Antibodies 101: Reading a Flow Plot

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    ...Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and...

18. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...PMID: 19678844. PubMed Central PMCID: PMC2906245. Danino, Tal, et al. "Programmable probiotics for detection...

19. Neuronal labeling with Spaghetti Monster

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    ...other advantage is that these tags are small (8-12 amino acids) and neither disturb POI folding nor its ability...

20. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...antidepressants-like effects in rats." Psychoneuroendocrinology 46 (2014): 78-87. PubMed PMID: 24882160...